FastQCFastQC Report
Mon 6 Jun 2016
ERR523115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10320954
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221980.2150770171052017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174250.1688312921460555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173450.042.922441
GTATCAA292300.037.3839871
TCAACGC343550.031.1884294
ATCAACG350150.030.6446913
CAACGCA350850.030.5116795
AACGCAG362650.029.5766356
TATCAAC371050.029.0873452
ACGCAGA413750.025.8444277
GTACATG378200.025.4587041
CGCAGAG430400.024.8327568
TACATGG388750.024.2321512
ACATGGG393300.023.195573
GCAGAGT477700.022.0976839
CATGGGG261250.021.2982564
GAGTACT224300.020.01381312-13
AGAGTAC400750.019.28341310-11
CAGAGTA459150.019.13280110-11
GTACTTT241650.018.49040614-15
GTATACG5800.017.8503571
ATGGGGG139850.017.3052795
Produced by FastQC (version 0.11.5)