FastQCFastQC Report
Mon 6 Jun 2016
ERR523115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10320954
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197610.19146485877177633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162950.15788269185193538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170700.040.6758881
GTATCAA277800.036.62761
TCAACGC337000.029.7994584
ATCAACG343850.029.1656533
CAACGCA345600.029.0171265
TATCAAC359250.028.1317982
AACGCAG357600.028.0822756
GTACATG365150.024.992781
ACGCAGA411850.024.2688677
TACATGG373700.023.935652
CGCAGAG431900.023.136858
ACATGGG382700.022.7542923
CATGGGG248800.020.4744664
GCAGAGT484250.020.4415079
GAGTACT227200.019.48835412-13
AGAGTAC402800.019.21926510-11
GTACTTT235350.018.64169314-15
CAGAGTA463600.017.72272910-11
AGTACTT245850.017.11139512-13
GTATAGG31450.016.7525751
Produced by FastQC (version 0.11.5)