FastQCFastQC Report
Mon 6 Jun 2016
ERR523114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6272024
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT534000.8513998033170791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179830.2867176528661242No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT147170.23464514804152534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136540.21769687105789134No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC108780.17343683633863646No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC101620.16202106369490932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85480.13628774379689873No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG80230.1279172401126016No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA79650.12699249875319354No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA72870.11618259113804412No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC67970.10837012103270013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54500.032.433532
GTACATG193950.029.1139641
GTACCTG64950.028.3762511
TACATGG198850.028.0862482
ACATGGG203250.026.6706093
CATGGGG133400.024.5623194
ACCTGGG71500.024.3293133
ATGGGGG81050.021.7509235
GAGTACT171300.019.12977612-13
AGAGTAC249400.018.82589110-11
TATAACG6900.017.7144282
AGTACTT182200.017.6114612-13
GAGTACA146600.017.446691
ATAACGC7400.017.1540343
TAACGCA7800.016.875474
GTACTTT194950.016.53472114-15
CATGGGA77750.016.5064874
AGTACAT146400.016.216352
GTGTAGC37750.015.6928451
TGGGGGG86250.015.5773476
Produced by FastQC (version 0.11.5)