FastQCFastQC Report
Mon 6 Jun 2016
ERR523114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6272024
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT551440.8792058193654871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168330.26838226384337815No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT147640.23539450741897672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133750.21324854624280776No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC112450.1792882170093737No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC105240.16779272528293895No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG85190.1358253731171947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84080.13405560948108616No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA81370.1297348351983347No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA77330.1232935333155613No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC70710.11273872676507615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG56550.034.4087222
GTACCTG66800.029.500731
GTACATG197350.027.5496371
ACCTGGG72800.026.5956863
TACATGG201300.026.477082
ACATGGG204900.025.2058333
CATGGGG137600.023.1214774
ATGGGGG79000.021.831545
GAGTACT180850.019.41701512-13
ATAACGC7000.018.7977283
AGAGTAC262300.018.71030610-11
CCTGGGG68300.018.439944
AGTACTT190350.017.67002512-13
GTACTTT199150.017.45316514-15
TATCACG4650.017.182912
TGGGGGG80000.016.917826
TATAACG6400.016.8907382
TATACCG5650.016.6351595
GAGTACA149350.016.3124711
TAACGCA7000.015.440994
Produced by FastQC (version 0.11.5)