FastQCFastQC Report
Mon 6 Jun 2016
ERR523113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13788298
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425120.3083194169432659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343850.24937813209433102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211780.1535940113855967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG187500.047.004332
GTACCTG217450.041.1661871
ACCTGGG224200.038.491963
CCTGGGG209100.026.5595764
GTATCAA597950.024.0487121
GGTATCA438750.023.4473041
GTACATG541050.022.839361
CATGGGG305250.022.4454964
TACATGG553300.021.7169972
TCAACGC670500.021.0330854
ACATGGG553250.021.0049113
CAACGCA678000.020.8197845
ATCAACG678450.020.788743
TATCAAC691300.020.4567382
AACGCAG693050.020.4228826
GAGTACT373850.019.82810612-13
TATCACG15100.018.9986382
ATGGGGG184850.018.5694355
ACGCAGA763950.018.4781487
CGCAGAG785350.017.9620178
Produced by FastQC (version 0.11.5)