FastQCFastQC Report
Mon 6 Jun 2016
ERR523113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13788298
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385720.2797444615716893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337500.24477277761185606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210930.15297754661235202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG199950.047.2434652
GTACCTG228550.041.9460071
ACCTGGG239850.038.4403233
CCTGGGG211450.028.402134
GGTATCA454400.022.9083541
GTATCAA609650.022.8278871
GTACATG548100.021.3682291
CATGGGG296700.020.2572634
CTGGGGG130500.020.2373475
TACATGG562550.020.0919042
TCAACGC692650.019.702014
ACATGGG557200.019.6841873
CAACGCA699400.019.5118645
ATCAACG701400.019.4227283
AACGCAG713750.019.2512536
TATCAAC712350.019.2248632
GAGTACT391700.018.4571412-13
TATAACG11850.018.2434352
ATGGGGG172450.017.848675
ACGCAGA794350.017.3097257
Produced by FastQC (version 0.11.5)