FastQCFastQC Report
Mon 6 Jun 2016
ERR523112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213934
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT627290.8695532839640618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247890.343626653640025No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT186040.2578898004888872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181530.2516380105501381No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC134830.18690218125089583No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC125650.17417680838222252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111710.15485309402608896No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG101550.14076923908646793No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA97880.1356818623513883No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA90190.1250219367130334No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC80630.11176980549031915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG266850.028.278781
TACCTGG56600.027.8273682
TACATGG278200.026.8540942
ACATGGG279100.025.8924353
CATGGGG195750.024.8095054
ATGGGGG127700.024.224995
GTACCTG70600.023.5749821
TATCACG5650.022.4677222
ACCTGGG73050.020.9180893
TATAACG5350.020.2123952
GAGTACT206850.019.4657612-13
TGGGGGG133450.019.4338596
TAACGCA6400.019.09914
AGAGTAC327300.019.0831110-11
AGTACTT217300.018.03235412-13
GTATATA36250.017.7690961
GTACTTT227050.017.51587914-15
GAGTACA207850.017.440431
AGTACAT202050.016.5445332
ATAACGC8500.016.0412393
Produced by FastQC (version 0.11.5)