FastQCFastQC Report
Mon 6 Jun 2016
ERR523112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213934
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT632540.8768308664869958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217990.3021790884141718No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT185090.25657290460378485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176060.24405546266433822No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC140240.19440155676500506No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC132140.18317328658676388No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG103880.14399909952045584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103380.14330599642303354No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100330.13907806752875754No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA91580.1269487633238674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG259850.028.5570221
TACCTGG52750.028.423862
TACATGG270950.026.9746672
ACATGGG267700.026.4028663
CATGGGG179700.025.1058394
ATGGGGG115700.023.8419255
GTACCTG69950.022.1175381
TATCACG6200.021.9847322
GAGTACT210650.020.30968312-13
ACCTGGG72150.020.061623
TGGGGGG112400.019.4830156
TATAACG4650.019.2050512
AGAGTAC328200.019.01610-11
GTACTTT229050.018.43835614-15
AGTACTT220600.018.36001412-13
GTATAGG21950.017.1389451
GAGTACA204450.017.0895391
AGTACAT197550.016.0836452
GTATTAG23550.015.5751521
GGGTTAG25400.015.3664321
Produced by FastQC (version 0.11.5)