FastQCFastQC Report
Mon 6 Jun 2016
ERR523111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575471
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193700.34741459510774964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162710.2918318470313988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96680.1734023905783027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG81200.048.4688872
GTACCTG97000.041.258181
ACCTGGG95150.040.621933
CCTGGGG89600.030.2782044
GTATCAA260350.026.426461
TATAACG5250.025.9736062
GGTATCA188950.024.3164371
ATAACGC6400.024.2455183
GTACATG250150.024.214061
CATGGGG149600.024.137484
ATGGGGG96550.024.1043645
TACATGG254350.023.4227812
TGGGGGG103650.022.6230556
TCAACGC301500.022.5030484
ACATGGG259400.022.3869273
CAACGCA304550.022.2612445
ATCAACG305100.022.1468543
TATCAAC307350.022.0457232
AACGCAG312450.021.717346
TAACGCA6550.020.8170414
Produced by FastQC (version 0.11.5)