FastQCFastQC Report
Mon 6 Jun 2016
ERR523111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575471
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170780.30630596051885123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159600.28625384294887374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94070.16872117171804857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84200.049.0071642
GTACCTG97000.043.0905951
ACCTGGG98600.040.7023123
TATCACG4700.032.998422
CCTGGGG91200.032.1534734
TATAACG6200.028.8050752
ATAACGC7150.027.6045973
TAACGCA7100.027.1371144
GGTATCA200150.025.8868031
GTATCAA273900.024.9588621
CTGGGGG57950.023.9222955
ATGGGGG95450.023.1396185
CATGGGG157250.023.0410294
GTACATG257000.022.959531
TGGGGGG94300.022.9234716
TACATGG259800.022.2325782
TCAACGC315300.021.2685644
ACATGGG264550.021.2452373
CAACGCA317550.021.1472745
TATCAAC320750.021.0702322
Produced by FastQC (version 0.11.5)