##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523111_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5575471
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11030225069774	33.0	31.0	34.0	30.0	34.0
2	32.24587088696184	34.0	31.0	34.0	30.0	34.0
3	32.42618533931932	34.0	31.0	34.0	30.0	35.0
4	35.53566021597099	37.0	35.0	37.0	33.0	37.0
5	35.474563852991075	36.0	35.0	37.0	33.0	37.0
6	35.60252362535829	37.0	35.0	37.0	33.0	37.0
7	35.44488366991775	36.0	35.0	37.0	33.0	37.0
8	35.430164554707574	36.0	35.0	37.0	33.0	37.0
9	36.69911367129342	37.0	35.0	39.0	33.0	39.0
10-11	36.499128414442474	37.5	35.0	39.0	32.5	39.0
12-13	36.50394208848006	37.5	35.0	39.0	33.0	39.0
14-15	37.643962814980114	38.0	37.0	41.0	33.0	41.0
16-17	37.45614191159814	38.0	36.5	41.0	32.5	41.0
18-19	37.347901190769356	38.0	36.0	41.0	32.0	41.0
20-21	37.37258448658418	38.0	36.0	40.0	32.5	41.0
22-23	37.21604049236379	38.0	35.5	40.0	32.0	41.0
24-25	37.13594985966208	38.0	36.5	40.0	32.0	41.0
26-27	36.81600191266352	38.0	36.0	40.0	31.5	41.0
28-29	36.75345006726786	38.0	35.5	40.0	31.0	41.0
30-31	36.50458176537911	37.5	35.5	40.0	31.0	41.0
32-33	36.44141104850155	37.5	36.0	40.0	30.5	41.0
34-35	36.206143660329325	37.5	35.0	40.0	30.5	41.0
36-37	35.98040488417929	37.0	35.0	40.0	30.0	41.0
38-39	35.92409834075005	37.0	35.0	40.0	30.0	41.0
40-41	36.110021287887605	37.5	35.0	40.0	30.0	41.0
42-43	36.14278345273431	37.5	35.0	40.0	30.0	41.0
44-45	35.97193205739928	37.0	35.0	40.0	30.0	41.0
46-47	35.84877349375506	37.0	35.0	40.0	28.5	41.0
48-49	35.62010653449727	37.5	34.5	40.0	28.5	41.0
50-51	35.4495934962266	37.5	34.0	40.0	27.5	41.0
52-53	35.44182509423867	37.5	35.0	40.0	27.5	41.0
54-55	35.242608830715824	37.5	34.0	40.0	27.0	41.0
56-57	34.9975073854747	37.0	34.0	40.0	26.5	41.0
58-59	34.80492150349271	37.0	34.0	39.0	26.5	41.0
60-61	34.607480874709964	36.5	33.5	39.0	26.0	41.0
62-63	34.35758521567057	36.5	33.0	39.0	26.0	41.0
64-65	34.1636849155883	36.5	33.0	38.5	25.0	40.0
66-67	33.6778768107663	35.5	33.0	38.0	25.0	40.0
68-69	33.54157648743936	35.5	32.5	37.0	23.5	40.0
70-71	33.12179580882045	35.0	32.5	37.0	24.0	39.5
72-73	32.89227968363569	35.0	32.0	37.0	23.5	39.0
74-75	32.69027666003464	35.0	31.5	37.0	22.5	39.0
76-77	31.1355085516542	34.0	29.5	36.0	21.0	37.0
78-79	32.260755817759616	35.0	31.5	36.5	23.0	37.0
80-81	32.37528336171061	35.0	32.0	36.0	23.0	37.5
82-83	32.27742337822222	35.0	32.0	37.0	23.5	37.0
84-85	31.94323824839193	35.0	31.5	35.5	22.5	36.5
86-87	31.816281889009915	35.0	31.0	36.0	22.5	36.0
88-89	31.7557967748375	35.0	31.0	36.0	22.0	37.0
90-91	31.51421279027368	34.5	31.0	35.0	21.5	36.5
92-93	31.28968862002869	34.0	30.5	35.0	21.0	36.5
94-95	31.399672691329577	34.0	31.0	35.0	20.5	36.5
96-97	31.12540303769852	34.0	30.5	35.0	20.0	37.0
98-99	30.966096855315	34.0	30.0	35.0	20.0	36.0
100	29.720277802539016	33.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08355019483373383
1101	2	-0.17193511047343435
1101	3	-0.17488244760177452
1101	4	-0.08798281501869099
1101	5	-0.2781864088157917
1101	6	-0.08712162594189721
1101	7	-0.08162753701222414
1101	8	-0.220807017254387
1101	9	-0.16624024101401602
1101	10-11	-0.16184738463280013
1101	12-13	-0.1735920819164889
1101	14-15	-0.34988960488430365
1101	16-17	-0.3514174130663932
1101	18-19	-0.3964081288705614
1101	20-21	-0.3344698824348953
1101	22-23	-0.8187308523373957
1101	24-25	-0.39932806667601994
1101	26-27	-0.47403623317699584
1101	28-29	-0.45504479598302083
1101	30-31	-0.3872041898384566
1101	32-33	-0.3856562440251494
1101	34-35	-0.5190646743369669
1101	36-37	-0.5365915347270871
1101	38-39	-0.41198179308503313
1101	40-41	-0.5009009971471556
1101	42-43	-0.5178249525398471
1101	44-45	-0.5548077060272476
1101	46-47	-0.49468627047589564
1101	48-49	-0.5992372222688331
1101	50-51	-0.5179166918590496
1101	52-53	-0.4271282038622246
1101	54-55	-0.5526422404014895
1101	56-57	-0.5664778631194096
1101	58-59	-0.6539858676415591
1101	60-61	-0.856847654584989
1101	62-63	-0.6517931789942324
1101	64-65	-0.6825406856713485
1101	66-67	-0.5551314740827991
1101	68-69	-0.6806824767494888
1101	70-71	-0.7542116724117847
1101	72-73	-0.703603891272806
1101	74-75	-0.7119107415673795
1101	76-77	-0.7613323880008203
1101	78-79	-0.6854761647509378
1101	80-81	-0.6328907325392557
1101	82-83	-0.693889212160343
1101	84-85	-0.6639042657274956
1101	86-87	-0.7203485206530438
1101	88-89	-0.7371706743104163
1101	90-91	-0.7692529403085757
1101	92-93	-0.7622560055933896
1101	94-95	-0.8369028484442005
1101	96-97	-0.922116533052769
1101	98-99	-0.8284421803209199
1101	100	-0.7996975578682033
1102	1	-0.026766879921062525
1102	2	-0.06644363421626309
1102	3	-0.13651166120011737
1102	4	-0.096762806693512
1102	5	-0.10314606448952901
1102	6	-0.17246031664633676
1102	7	-0.08350172833147695
1102	8	-0.23081801497077237
1102	9	-0.1933790029404534
1102	10-11	-0.2105806650622668
1102	12-13	-0.18633403680329508
1102	14-15	-0.2772822404470432
1102	16-17	-0.25681133499969633
1102	18-19	-0.22612243430906886
1102	20-21	-0.30060166638205743
1102	22-23	-0.3252652319916791
1102	24-25	-0.21053965412700393
1102	26-27	-0.18938175558902515
1102	28-29	-0.2856549300129885
1102	30-31	-0.3735947037783518
1102	32-33	-0.31183463669079714
1102	34-35	-0.3126658010390031
1102	36-37	-0.3818898571823226
1102	38-39	-0.3324654841346444
1102	40-41	-0.35647828840072293
1102	42-43	-0.4461711222382334
1102	44-45	-0.2786983481257366
1102	46-47	-0.3264700125838971
1102	48-49	-0.3424433983512216
1102	50-51	-0.29331711057881193
1102	52-53	-0.3775196002167718
1102	54-55	-0.4040238755138006
1102	56-57	-0.5372300720609857
1102	58-59	-0.4178321907885021
1102	60-61	-0.460317676751302
1102	62-63	-0.4443780251116394
1102	64-65	-0.4473327679990575
1102	66-67	-0.5770879295509346
1102	68-69	-0.45329847053226047
1102	70-71	-0.4816764014571788
1102	72-73	-0.6194428926336855
1102	74-75	-0.5284987188556229
1102	76-77	-0.4726772262168275
1102	78-79	-0.6046012208844012
1102	80-81	-0.5442670465066293
1102	82-83	-0.4513065191073622
1102	84-85	-0.43492160917408285
1102	86-87	-0.44652194076512686
1102	88-89	-0.5166651792115431
1102	90-91	-0.6149895651647803
1102	92-93	-0.5354630155610494
1102	94-95	-0.7152547596477241
1102	96-97	-0.7326981611932517
1102	98-99	-0.8212318922272317
1102	100	-0.7792231113253756
1103	1	-0.09818166630002167
1103	2	-0.1396237750223861
1103	3	-0.24741606601306643
1103	4	-0.08637047654233498
1103	5	-0.17669041533452656
1103	6	-0.19446983242789173
1103	7	-0.23600701699874804
1103	8	-0.24292649699166446
1103	9	-0.26704731506286805
1103	10-11	-0.3883488453898849
1103	12-13	-0.2359830458283696
1103	14-15	-0.29578050964205005
1103	16-17	-0.35841185565094946
1103	18-19	-0.4313001892251904
1103	20-21	-0.40989874135426163
1103	22-23	-0.4079515013461332
1103	24-25	-0.3548135667435446
1103	26-27	-0.43376997300363485
1103	28-29	-0.3612335131987905
1103	30-31	-0.5139644264481475
1103	32-33	-0.35562588506627435
1103	34-35	-0.4750694845153731
1103	36-37	-0.5163583633093438
1103	38-39	-0.4071398097332093
1103	40-41	-0.4976082374592323
1103	42-43	-0.5014750919575093
1103	44-45	-0.3387498090701513
1103	46-47	-0.5438403834635395
1103	48-49	-0.5405823923467779
1103	50-51	-0.45447416722557676
1103	52-53	-0.5228503955731654
1103	54-55	-1.269376389441561
1103	56-57	-0.520980363213738
1103	58-59	-0.4292645109172142
1103	60-61	-0.36843664041002455
1103	62-63	-0.3531189827093826
1103	64-65	-0.3461020477972312
1103	66-67	-0.38662873600620173
1103	68-69	-0.48066026620823976
1103	70-71	-0.5079595604044229
1103	72-73	-0.6740706830992593
1103	74-75	-0.6524030678339088
1103	76-77	-0.4243852806720092
1103	78-79	-0.2998324201253837
1103	80-81	-1.1996200416958231
1103	82-83	-0.28424130130843395
1103	84-85	-0.15552565060339774
1103	86-87	-0.2745561378273038
1103	88-89	-0.34486243570193764
1103	90-91	-0.27552150279829846
1103	92-93	-0.2610524878943501
1103	94-95	-0.3103058191937187
1103	96-97	-0.4001140470427629
1103	98-99	-0.3434138947442342
1103	100	-0.42023600899280567
1104	1	-0.14350505881992603
1104	2	-0.24108010803782776
1104	3	-0.10223401971704504
1104	4	-0.03319427832959576
1104	5	-0.1022349972918235
1104	6	-0.2285208423700027
1104	7	-0.18989765458142926
1104	8	-0.11002542559577222
1104	9	-0.12922386354258464
1104	10-11	-0.12804358508616787
1104	12-13	-0.2006249780660596
1104	14-15	-0.3006143127994818
1104	16-17	-0.3272826801434192
1104	18-19	-0.25137396770941933
1104	20-21	-0.3220505436332175
1104	22-23	-0.27093714447484984
1104	24-25	-0.17361724965735448
1104	26-27	-0.25328553153005373
1104	28-29	-0.4339156770711057
1104	30-31	-0.36030573250084785
1104	32-33	-0.2319600957932053
1104	34-35	-0.2719325491425977
1104	36-37	-0.37672415205607734
1104	38-39	-0.2082759107812393
1104	40-41	-0.16444292927268123
1104	42-43	-0.04734408939086876
1104	44-45	-0.25603185955987584
1104	46-47	-0.25481801884053823
1104	48-49	-0.2626183959890085
1104	50-51	-0.23911549502775387
1104	52-53	-0.30820349711679995
1104	54-55	-0.3603008243071031
1104	56-57	-0.3004166863578632
1104	58-59	-0.23496966897625526
1104	60-61	-0.35169695193539496
1104	62-63	-0.30848575026811176
1104	64-65	-0.6249103013300825
1104	66-67	-0.27659113366601673
1104	68-69	-0.15846030094630947
1104	70-71	-0.2016154325208106
1104	72-73	-0.1955373118924939
1104	74-75	-0.33441800452184367
1104	76-77	-0.24942055780313765
1104	78-79	-0.16628776074600893
1104	80-81	-0.2475457328024504
1104	82-83	-0.25831648527500306
1104	84-85	-0.894746400806337
1104	86-87	-0.32391804239678734
1104	88-89	-0.30598220680425214
1104	90-91	-0.34176851691237786
1104	92-93	-0.30042108709639237
1104	94-95	-0.3285756633121828
1104	96-97	-0.22456918493979927
1104	98-99	-0.21419509197815856
1104	100	-1.5475508399214988
1105	1	-0.012306281733550861
1105	2	-0.1488798392054136
1105	3	-0.17111936549728313
1105	4	-0.061673689462971026
1105	5	-0.06402484984395329
1105	6	-0.06445429380331547
1105	7	-0.11538424305154393
1105	8	-0.13968780429199512
1105	9	-0.19094718851773962
1105	10-11	-0.18891436662367767
1105	12-13	-0.11798642909860035
1105	14-15	-0.3332043640324329
1105	16-17	-0.2886038551808028
1105	18-19	-0.29337828601657634
1105	20-21	-0.2681900501747094
1105	22-23	-0.2896042545493742
1105	24-25	-0.19617714948391551
1105	26-27	-0.2815194121211704
1105	28-29	-0.24958281485861988
1105	30-31	-0.3257725414384609
1105	32-33	-0.27558590285207885
1105	34-35	-0.25543782600768594
1105	36-37	-0.33367884197148356
1105	38-39	-0.3885244356384021
1105	40-41	-0.3080597173107691
1105	42-43	-0.2797119611462975
1105	44-45	-0.36868493898973753
1105	46-47	-0.5039118778220768
1105	48-49	-0.4287938375889624
1105	50-51	-0.2525430951663523
1105	52-53	-0.36752869726117154
1105	54-55	-1.1581214189294968
1105	56-57	-0.31425061798023535
1105	58-59	-0.3291156849550063
1105	60-61	-0.372161159295473
1105	62-63	-0.2880869390078473
1105	64-65	-0.2460732368278471
1105	66-67	-0.405664832676365
1105	68-69	-0.1640043094222179
1105	70-71	-0.2270365265017915
1105	72-73	-0.2684926629841158
1105	74-75	-0.1928188370266568
1105	76-77	-0.27346895682036276
1105	78-79	-0.18342044021674297
1105	80-81	-0.14572756380469798
1105	82-83	-0.14109730519582087
1105	84-85	-0.13504768201563166
1105	86-87	-0.11506098682502497
1105	88-89	-0.26555018258998686
1105	90-91	-0.16381902244489055
1105	92-93	-0.037964400943724996
1105	94-95	-0.25048347153762407
1105	96-97	-0.23897262672931774
1105	98-99	-0.1749307029914
1105	100	-0.04983502992241995
1106	1	-0.07776107330013815
1106	2	-0.16501060500730347
1106	3	-0.16625560874923906
1106	4	-0.15854079657303544
1106	5	-0.12313480833035584
1106	6	-0.1690146365078533
1106	7	-0.1534916803468107
1106	8	-0.221924910895261
1106	9	-0.13927664639606974
1106	10-11	-0.255748470433403
1106	12-13	-0.18899888382491525
1106	14-15	-0.27429705937860405
1106	16-17	-0.19955482075766184
1106	18-19	-0.2666977334497176
1106	20-21	-0.2515595474050585
1106	22-23	-0.19784967164370215
1106	24-25	-0.09640347540846506
1106	26-27	-0.21722999343295157
1106	28-29	-0.20692405356964372
1106	30-31	-0.32447509383077033
1106	32-33	-0.16575022959236207
1106	34-35	-0.2117321170665818
1106	36-37	-0.19649396860017276
1106	38-39	-0.2990344396879223
1106	40-41	-0.2668040860811658
1106	42-43	-0.3045711065319594
1106	44-45	-0.30426134066659927
1106	46-47	-0.4187922649433631
1106	48-49	-0.32029677093680675
1106	50-51	-0.30470063148123927
1106	52-53	-0.3772020013427948
1106	54-55	-0.4326046362744407
1106	56-57	-0.32915679829066136
1106	58-59	-0.4385920184491212
1106	60-61	-0.4163726446641931
1106	62-63	-0.4389871504537055
1106	64-65	-0.3760284395796418
1106	66-67	-0.2984595465784139
1106	68-69	-0.2334045683903696
1106	70-71	-0.27356938270160924
1106	72-73	-0.2674231604591242
1106	74-75	-0.24449540241659662
1106	76-77	-0.3663810596874235
1106	78-79	-0.23189176873113837
1106	80-81	-0.1561026595981403
1106	82-83	-0.2624362226817425
1106	84-85	-0.1226016020055809
1106	86-87	-0.11799558141778022
1106	88-89	-0.22164787546774178
1106	90-91	-0.18167294491215102
1106	92-93	-0.13473453667289093
1106	94-95	-0.14732912480235782
1106	96-97	-0.056081606920376004
1106	98-99	-0.07392956132299844
1106	100	-0.03460619996980796
1107	1	-0.05245372328802489
1107	2	-0.12460632361531054
1107	3	-0.1324888585802455
1107	4	-0.061027430778622715
1107	5	-0.10796521908227419
1107	6	-0.18714729314700662
1107	7	-0.25163980841263367
1107	8	-0.15032112401055286
1107	9	-0.29571084403270476
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2313	1	0.2236530895100941
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2313	100	0.48832443171946593
2314	1	-1.9887809857002985
2314	2	4.399920970143967E-4
2314	3	-0.016908592263199296
2314	4	-0.06130430712483559
2314	5	-0.020355571298843245
2314	6	-8.373355063753252E-4
2314	7	0.057840084799963165
2314	8	0.06472832691922292
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2314	10-11	-0.8299215754762486
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2314	86-87	0.32695069759673956
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2314	90-91	0.3861807921344145
2314	92-93	0.21000194441015907
2314	94-95	0.03223280908619941
2314	96-97	0.17721519859660972
2314	98-99	0.18122755290143644
2314	100	0.16865018514825536
2315	1	0.1201844643352814
2315	2	0.05798252792402536
2315	3	0.06460151864440178
2315	4	-0.13194401321857896
2315	5	-0.006843148416159295
2315	6	-0.026674673095726575
2315	7	0.030524972087135893
2315	8	0.029512849894821613
2315	9	0.06482625108192508
2315	10-11	0.10316014506086901
2315	12-13	0.060604486146523584
2315	14-15	0.07596574107294884
2315	16-17	0.09946930270012189
2315	18-19	0.21054687177609566
2315	20-21	0.19590199581110568
2315	22-23	0.22147667848362573
2315	24-25	0.28144011865370544
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2315	98-99	0.476372001118861
2315	100	0.2644003491306073
2316	1	0.033716201535881396
2316	2	-0.12204455001025849
2316	3	-0.06716776846069195
2316	4	0.0019658487413494186
2316	5	-0.026271228634513477
2316	6	-0.049113598866178165
2316	7	0.017556308759473893
2316	8	0.07246117450148404
2316	9	-0.06355142521149304
2316	10-11	0.04190299858127844
2316	12-13	0.07331522627071507
2316	14-15	0.018076775040107407
2316	16-17	-1.1892210697134544
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2316	22-23	0.1305704135067458
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2316	30-31	0.15721989113883694
2316	32-33	0.23576521586937815
2316	34-35	0.27696053478508276
2316	36-37	0.19468156060720077
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2316	52-53	0.230014039736524
2316	54-55	0.34204148909802257
2316	56-57	0.2799812700569859
2316	58-59	0.22556654768862927
2316	60-61	-1.1511103678827652
2316	62-63	0.142169881589588
2316	64-65	0.2611834039424181
2316	66-67	0.0793469698033391
2316	68-69	0.25194031300524955
2316	70-71	0.22840328517345654
2316	72-73	0.33941597463856965
2316	74-75	0.2359437047076085
2316	76-77	-0.11295239768678655
2316	78-79	-0.18039389105104675
2316	80-81	0.037754874050946796
2316	82-83	0.03775270573571987
2316	84-85	-0.5738265234413227
2316	86-87	0.12371557725985127
2316	88-89	0.10657590448394316
2316	90-91	0.22995961040335544
2316	92-93	0.421739647123065
2316	94-95	0.42152441756329395
2316	96-97	0.40873223517264634
2316	98-99	0.37162680211706345
2316	100	0.2363803977528356
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	0.0
11	7.0
12	35.0
13	211.0
14	837.0
15	2260.0
16	4864.0
17	9120.0
18	14845.0
19	21050.0
20	27659.0
21	36111.0
22	45309.0
23	52631.0
24	57651.0
25	64895.0
26	74514.0
27	89037.0
28	106159.0
29	130073.0
30	158312.0
31	195328.0
32	242847.0
33	315147.0
34	448747.0
35	785236.0
36	1046258.0
37	808256.0
38	712680.0
39	125347.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86784891512785	18.101981086488863	13.035332698253544	23.99483730012975
2	17.015569410497683	20.301866973183046	38.98037025198273	23.702193364336548
3	19.63371345667478	25.56076428341211	29.632025706886466	25.17349655302664
4	13.564979391834934	16.459377342856016	36.1195130087921	33.856130256516956
5	13.98911261401112	36.36322710141598	33.30359578602095	16.34406449855195
6	31.22914638063762	34.787052071475216	18.94257902157504	15.041222526312126
7	28.208845494846983	31.930862881360156	21.494309628729123	18.365981995063734
8	26.8852063254287	34.155614671711625	20.22308815912003	18.736090843739643
9	27.556703281211583	15.83053700754609	19.938602496542444	36.67415721469989
10-11	25.88854160379828	25.205935534947233	27.832547943711656	21.072974917542833
12-13	26.059709381592235	23.11662248940916	27.71143989939477	23.11222822960384
14-15	23.65672445643444	24.47196853502441	26.039405481796003	25.83190152674515
16-17	23.12414097768062	26.934384191639708	26.661165097475887	23.28030973320379
18-19	22.855754376896364	26.10548099182329	28.482940179561577	22.55582445171877
20-21	23.71817136402284	25.46700712510897	28.285591220110902	22.529230290757283
22-23	23.394416552706776	25.26806693622452	28.19196881171333	23.145547699355376
24-25	23.005157016687864	25.316411309007652	28.313871767739368	23.364559906565113
26-27	23.041228019270722	25.287006416009987	28.336290661316422	23.335474903402872
28-29	23.025188347332044	25.92573591542064	28.24234598901958	22.806729748227742
30-31	23.788034246292767	25.43172894270939	28.229567831588316	22.550668979409533
32-33	23.441937727732732	25.481698333257402	28.1498102443989	22.92655369461097
34-35	23.50216126878621	25.73850674605011	28.05291547966391	22.706416505499767
36-37	23.617702923310333	25.69479302414701	27.862642143914567	22.824861908628087
38-39	23.492821005519296	25.93797220344133	27.727987859217652	22.84121893182173
40-41	23.591005732826073	25.60162477454738	27.94385681450093	22.863512678125623
42-43	23.316144658229906	25.79114705953837	27.607836686988925	23.284871595242798
44-45	23.12957487050208	25.89098345416697	27.76283681185746	23.216604863473492
46-47	23.189324294301773	25.692329646128627	27.44308891060828	23.675257148961318
48-49	23.23622203043611	25.87525834169433	27.427739220238166	23.460780407631397
50-51	23.218833521959876	26.18340061282549	27.270061729225564	23.327704135989066
52-53	23.24565781749806	26.31949835129132	26.876852919400502	23.557990911810116
54-55	23.030684369433544	25.984139901195448	27.333317310132692	23.651858419238316
56-57	23.282147687794346	26.136005112585757	26.930999284159075	23.65084791546082
58-59	23.253658335336343	26.190452273925818	27.14161946137537	23.41426992936247
60-61	23.595153345067725	26.09456571385064	27.222046398697263	23.088234542384374
62-63	23.446766044942628	26.17680788540649	27.141382313651384	23.235043755999502
64-65	23.2601608692954	25.965086635035185	27.006803745988222	23.767948749681192
66-67	23.116406093131367	25.978071090717652	26.975510658334507	23.930012157816474
68-69	22.9058936566592	26.054624283625287	27.09899382455539	23.940488235160124
70-71	23.01731641562913	25.94512812483129	27.122501619148622	23.915053840390957
72-73	22.971107605176254	26.264961505265717	26.717097961224745	24.046832928333288
74-75	23.01308792410645	26.355950102037113	26.558688041770868	24.07227393208557
76-77	22.89631103952808	25.994959632306603	26.882265953877614	24.226463374287704
78-79	22.73174661068987	26.13107864501672	26.92434729587439	24.21282744841902
80-81	22.869402688599898	26.296523681334676	26.590104470172633	24.243969159892796
82-83	23.02631012551521	26.344912132180177	26.568536540744915	24.060241201559705
84-85	22.956411364058056	26.54063869759161	26.3321086501363	24.170841288214028
86-87	22.73340634981531	26.44802794965468	26.545571584445998	24.27299411608401
88-89	22.520975847732743	26.23472323359866	26.66124409465766	24.583056824010935
90-91	23.119206685957796	26.079606359777642	26.556258767795548	24.24492818646901
92-93	22.858389907313196	26.279248124319448	26.506817485175617	24.35554448319174
94-95	22.681572552345802	26.09080918903533	26.50344697335884	24.724171285260024
96-97	22.3888768617826	26.135740970687323	26.660916972490966	24.81446519503911
98-99	22.519786990936236	26.04661853357495	26.4611954188039	24.972399056684907
100	22.404853692946922	26.202921872360086	26.606995720178368	24.785228714514627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	903.0
1	727.5
2	1342.0
3	1957.0
4	1368.5
5	1087.0
6	1557.5
7	2946.5
8	4564.0
9	5458.0
10	5229.0
11	4635.5
12	4737.0
13	5182.0
14	5824.5
15	6408.0
16	6991.5
17	7543.5
18	8102.5
19	8812.5
20	9543.5
21	10432.5
22	11734.0
23	13507.0
24	15942.0
25	19319.5
26	23907.0
27	29439.5
28	34988.5
29	41242.0
30	49049.0
31	57076.0
32	65851.5
33	74848.5
34	83965.0
35	92457.0
36	102931.5
37	114302.5
38	122269.0
39	129228.0
40	135141.0
41	141748.0
42	149790.5
43	159001.0
44	168025.5
45	177398.5
46	188695.5
47	201952.0
48	218981.5
49	232554.5
50	242915.5
51	243611.5
52	232529.0
53	224075.5
54	217871.5
55	206414.0
56	190259.5
57	174422.0
58	159142.0
59	141184.5
60	118588.5
61	97232.5
62	79940.5
63	63357.5
64	48374.0
65	37233.5
66	28441.0
67	22801.0
68	18535.0
69	14476.5
70	11615.0
71	9080.0
72	7403.0
73	6349.0
74	5229.0
75	4207.0
76	3163.0
77	2158.5
78	1475.5
79	1018.0
80	731.0
81	502.0
82	310.0
83	187.5
84	122.5
85	91.5
86	65.0
87	42.0
88	24.0
89	16.5
90	12.5
91	8.5
92	3.5
93	0.5
94	0.5
95	0.5
96	1.0
97	1.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0433685333490211
2	0.010133673011661258
3	0.0
4	1.9729274889959968E-4
5	5.021997244717083E-4
6	0.0
7	0.0
8	1.2554993111792706E-4
9	0.0
10-11	8.967852222709077E-6
12-13	2.7800341890398136E-4
14-15	0.011434011583954072
16-17	0.0016500848089784702
18-19	3.5871408890836306E-5
20-21	8.519459611573624E-4
22-23	7.981388478211078E-4
24-25	0.004510829668022666
26-27	0.016151101853099047
28-29	0.013102032097377963
30-31	0.010061930193879584
32-33	0.0072639603003943525
34-35	0.009057530744936168
36-37	0.004797800939149357
38-39	0.003811337194651358
40-41	0.005335872072511901
42-43	0.006914214063708699
44-45	0.006842471245927025
46-47	0.014931473950810611
48-49	0.01746937612983728
50-51	0.0172900190853831
52-53	0.011720982855080763
54-55	0.00398172638688283
56-57	0.0037664979335378123
58-59	0.00952385906051704
60-61	0.0021612523856728875
62-63	1.2554993111792706E-4
64-65	2.33164157790436E-4
66-67	8.160745522665261E-4
68-69	3.5871408890836306E-5
70-71	1.7935704445418154E-4
72-73	0.002923519824603159
74-75	0.0052641292547302284
76-77	0.005066836505830629
78-79	0.0033091374701796493
80-81	7.174281778167261E-5
82-83	9.147209267163259E-4
84-85	2.7800341890398136E-4
86-87	3.138748277948177E-4
88-89	1.9729274889959968E-4
90-91	4.3942475891274477E-4
92-93	5.021997244717083E-4
94-95	0.0
96-97	0.0018294418534326518
98-99	4.932318722489993E-4
100	8.967852222709077E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5575471.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.66751594018657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00892640580298	44.00587385568727
2	15.263302070783475	17.90920035075144
3	4.549227788716966	8.006756814302769
4	1.8672770959024583	4.3819403515441095
5	0.9093748214372745	2.667538091613781
6	0.559779299232707	1.9704516576432778
7	0.36289569151231793	1.4903132136496757
8	0.2384802767607715	1.1192836350626132
9	0.18903938322793937	0.9981423925963353
>10	0.9686100456303338	10.158042993352378
>50	0.05547753977965693	2.206879966112888
>100	0.025411287618406823	2.702307310932006
>500	0.0013265566939073044	0.5424679189914479
>1k	7.580320875978871E-4	0.8969974077750671
>5k	3.790160437986258E-5	0.2091733915452759
>10k+	7.580320875972516E-5	0.7346306484397603
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17078	0.30630596051885123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15960	0.28625384294887374	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9407	0.16872117171804857	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7935704445418153E-5
2	0.0	0.0	0.0	0.0	1.7935704445418153E-5
3	0.0	0.0	0.0	0.0	1.7935704445418153E-5
4	0.0	0.0	0.0	0.0	3.5871408890836306E-5
5	0.0	0.0	0.0	0.0	3.5871408890836306E-5
6	0.0	0.0	0.0	0.0	3.5871408890836306E-5
7	0.0	0.0	0.0	0.0	3.5871408890836306E-5
8	0.0	0.0	0.0	0.0	5.380711333625446E-5
9	0.0	0.0	0.0	1.7935704445418153E-5	7.174281778167261E-5
10-11	0.0	0.0	0.0	5.380711333625446E-5	7.174281778167261E-5
12-13	0.0	0.0	0.0	6.277496555896353E-5	7.174281778167261E-5
14-15	0.0	0.0	0.0	7.174281778167261E-5	1.16582078895218E-4
16-17	0.0	0.0	0.0	8.071067000438169E-5	1.7935704445418154E-4
18-19	0.0	0.0	0.0	1.0761422667250892E-4	1.972927488995997E-4
20-21	0.0	0.0	0.0	1.16582078895218E-4	1.972927488995997E-4
22-23	0.0	0.0	0.0	1.9729274889959968E-4	2.421320100131451E-4
24-25	0.0	0.0	0.0	4.394247589127448E-4	3.2284268001752677E-4
26-27	0.0	0.0	0.0	9.774958922752893E-4	3.407783844629449E-4
28-29	0.0	0.0	0.0	0.002681387814590014	3.407783844629449E-4
30-31	0.0	0.0	0.0	0.009676312548303093	3.407783844629449E-4
32-33	0.0	0.0	0.0	0.02788105256040252	3.407783844629449E-4
34-35	0.0	0.0	0.0	0.055869719347477545	3.587140889083631E-4
36-37	0.0	0.0	0.0	0.10461896403012409	3.587140889083631E-4
38-39	0.0	0.0	0.0	0.18058564020869267	4.3942475891274477E-4
40-41	0.0	0.0	0.0	0.2733401357481727	4.66328315580872E-4
42-43	0.0	0.0	0.0	0.36630089188877496	4.842640200262902E-4
44-45	0.0	0.0	0.0	0.4778161342781624	5.111675766944174E-4
46-47	0.0	0.0	0.0	0.6085763875374833	5.8291039447609E-4
48-49	0.0	0.0	0.0	0.7472462864572338	6.456853600350535E-4
50-51	0.0	0.0	0.0	0.8980855608432006	6.456853600350535E-4
52-53	0.0	0.0	0.0	1.0566013167318062	7.084603255940171E-4
54-55	0.0	0.0	0.0	1.2416350116429626	7.443317344848533E-4
56-57	0.0	0.0	0.0	1.4464159171485242	7.532995867075624E-4
58-59	0.0	0.0	0.0	1.6688634915328229	7.532995867075624E-4
60-61	0.0	0.0	0.0	1.8978217266308084	7.532995867075624E-4
62-63	0.0	1.7935704445418153E-5	0.0	2.147495700363252	7.532995867075624E-4
64-65	0.0	1.7935704445418153E-5	0.0	2.3968199278590094	7.622674389302715E-4
66-67	0.0	1.7935704445418153E-5	0.0	2.678536037583193	7.802031433756897E-4
68-69	0.0	1.7935704445418153E-5	0.0	3.0118083297357297	7.891709955983988E-4
70-71	0.0	1.7935704445418153E-5	0.0	3.3617070199091703	8.071067000438169E-4
72-73	0.0	1.7935704445418153E-5	0.0	3.69101552137927	8.967852222709076E-4
74-75	0.0	1.7935704445418153E-5	0.0	4.033515733468975	9.147209267163258E-4
76-77	0.0	1.7935704445418153E-5	0.0	4.412667557592892	9.236887789390349E-4
78-79	0.0	1.7935704445418153E-5	0.0	4.8244713316596926	9.685280400525804E-4
80-81	8.967852222709077E-6	1.7935704445418153E-5	0.0	5.2464536179992685	9.954315967207075E-4
82-83	1.7935704445418153E-5	1.7935704445418153E-5	0.0	5.68980629618556	0.0011120136756159256
84-85	1.7935704445418153E-5	1.7935704445418153E-5	0.0	6.1404408703766915	0.0011209815278386346
86-87	1.7935704445418153E-5	1.7935704445418153E-5	0.0	6.6098720628266205	0.0011478850845067618
88	1.7935704445418153E-5	1.7935704445418153E-5	0.0	6.984808996405865	0.0012016921978430164
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	8420	0.0	49.007164	2
GTACCTG	9700	0.0	43.090595	1
ACCTGGG	9860	0.0	40.702312	3
TATCACG	470	0.0	32.99842	2
CCTGGGG	9120	0.0	32.153473	4
TATAACG	620	0.0	28.805075	2
ATAACGC	715	0.0	27.604597	3
TAACGCA	710	0.0	27.137114	4
GGTATCA	20015	0.0	25.886803	1
GTATCAA	27390	0.0	24.958862	1
CTGGGGG	5795	0.0	23.922295	5
ATGGGGG	9545	0.0	23.139618	5
CATGGGG	15725	0.0	23.041029	4
GTACATG	25700	0.0	22.95953	1
TGGGGGG	9430	0.0	22.923471	6
TACATGG	25980	0.0	22.232578	2
TCAACGC	31530	0.0	21.268564	4
ACATGGG	26455	0.0	21.245237	3
CAACGCA	31755	0.0	21.147274	5
TATCAAC	32075	0.0	21.070232	2
>>END_MODULE