FastQCFastQC Report
Mon 6 Jun 2016
ERR523110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5577264
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166580.2986769139850651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132100.23685448635746847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79770.14302711867324194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86650.046.915851
TACCTGG64050.041.0250632
GTATCAA153500.040.5839271
GTACCTG79650.033.7897261
TCAACGC185900.033.141084
ACCTGGG79000.032.6608623
CAACGCA189700.032.5016945
ATCAACG191400.032.117983
AACGCAG197950.031.4495186
TATCAAC197450.031.2582132
GTACATG186650.029.3426571
TACATGG192850.027.908882
ACGCAGA225400.027.5361087
ACATGGG197350.026.6486593
CGCAGAG235050.026.4244128
CATGGGG147500.024.9148394
ATGGGGG91100.023.677395
GCAGAGT258750.023.4585259
CCTGGGG81100.022.2512154
AGAGTAC228000.020.10023910-11
Produced by FastQC (version 0.11.5)