FastQCFastQC Report
Mon 6 Jun 2016
ERR523110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5577264
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154430.27689203882046826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129100.2314755048353458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78380.14053485723465842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92900.047.44731
TACCTGG66150.040.311392
GTATCAA155200.037.9489861
GTACCTG79750.033.6816251
ACCTGGG81050.032.469723
TCAACGC189550.030.5928064
CAACGCA193650.030.0178875
ATCAACG193050.029.9418623
TATCAAC197900.029.4442252
TATAACG4350.029.1910082
AACGCAG201550.029.1441426
GTACATG182750.028.4698621
TACATGG187900.027.2067932
ACGCAGA229850.025.5351317
ACATGGG194650.025.301743
TAACGCA6000.025.062634
CGCAGAG241600.024.2971748
CATGGGG143050.024.013614
CCTGGGG79200.022.6655434
ATGGGGG90950.022.1140865
Produced by FastQC (version 0.11.5)