FastQCFastQC Report
Mon 6 Jun 2016
ERR523109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8088047
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT712400.8808059597081965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282540.34933031422789707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223030.27575260133874097No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT204810.2532255314540086No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC157770.19506563203700475No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC144000.17804050841939964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124160.15351048281494903No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA117600.1453997485425097No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA102210.1263716692051864No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC92260.11406956463037368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG295250.027.8547021
TACATGG301900.027.0681322
TACCTGG61650.025.2444882
ACATGGG315200.025.0617223
CATGGGG221350.023.618584
ATGGGGG132000.022.4392625
GAGTACT231950.021.1460212-13
GTACCTG76250.020.7820641
TATAACG7050.020.0080052
ATAACGC6600.019.9481183
AGAGTAC367400.019.17237710-11
GTACTTT255150.019.0035614-15
ACCTGGG82150.018.201513
AGTACTT244750.018.1589612-13
GAGTACA228550.018.1256391
TGGGGGG127200.017.6178326
AGTACAT222800.017.3260542
CATGGGT57700.015.481264
ACTTTTT318700.015.46417416-17
GTATAGG20150.015.1683011
Produced by FastQC (version 0.11.5)