FastQCFastQC Report
Mon 6 Jun 2016
ERR523109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8088047
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT728190.9003285960133516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262430.32446646267016005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218820.2705473892523127No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT211850.2619297340878459No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC169220.20922232524118617No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC150680.18629960978218846No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA120370.148824555544744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119700.14799617262362594No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG117450.14521428967957284No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA107800.1332831028306339No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC95050.11751909948099955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG306850.026.56311
TACATGG312450.025.4393622
TACCTGG63600.025.2762182
ACATGGG320550.023.9404053
CATGGGG221250.021.9835664
GAGTACT242700.021.25989512-13
ATGGGGG128650.020.7115065
GTACCTG81400.020.512111
GTACTTT262750.019.4014414-15
ACCTGGG79850.019.3625283
AGAGTAC390150.018.9066710-11
TATAACG7100.018.5371422
AGTACTT254400.018.05526412-13
TGGGGGG117700.017.3281296
TATCACG7100.017.213062
GAGTACA240700.016.7655411
AGTACAT236400.016.0262032
ACTTTTT321100.015.93113116-17
CATGGGA104300.015.3191854
TATACTG36300.015.1466875
Produced by FastQC (version 0.11.5)