FastQCFastQC Report
Mon 6 Jun 2016
ERR523108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842559
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22250.038.445682
TATAACG1457.4396667E-1032.414412
GTACATG69700.031.2487531
ACCTGGG26750.030.7440683
TACATGG72600.029.7154432
GTACCTG30950.028.8786851
GTATCAA32300.028.5455251
ACATGGG75500.027.5742593
GGTATCA23550.027.5659311
CATGGGG62250.027.0243534
TGGGGGG31750.026.632416
ATGGGGG39050.026.231525
TCAACGC35200.025.4977784
CAACGCA36400.024.9140265
AACGCAG38800.023.3672336
ATCAACG38750.023.2850173
TATCAAC38600.023.2569242
GTATCGC1503.305431E-521.9224196
CCTGGGG29150.021.2787934
TATCACG1605.414326E-520.5628932
Produced by FastQC (version 0.11.5)