FastQCFastQC Report
Mon 6 Jun 2016
ERR523108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842559
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22450.041.4827772
TATAACG1209.640644E-1139.195642
ATAACGC1850.033.0227553
ACCTGGG28300.032.713083
GTATCAA31550.031.1678961
GTACCTG32100.030.1941491
TGGGGGG27700.029.8573326
GTACATG70850.029.0203171
TACATGG73050.027.3645112
TCAACGC35800.026.9091974
CAACGCA36300.026.409095
ATGGGGG36300.026.2796335
ACATGGG75700.026.0732483
CATGGGG59250.025.9351834
AACGCAG38850.025.158836
TATCAAC38800.024.9720672
ATCAACG38300.024.9080073
TATCACG1701.2358396E-724.9007592
CCTGGGG29050.023.7794114
GGTATCA22300.022.575611
Produced by FastQC (version 0.11.5)