FastQCFastQC Report
Mon 6 Jun 2016
ERR523107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737457
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT532640.9283555414881541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201060.35043399889532945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165470.2884030329116192No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT152580.2659366335991712No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC126070.21973149428396588No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC108750.18954390420703807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90810.15827569600957359No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA90720.1581188320888505No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG86980.1516002647165809No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA78430.13669819224788962No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC68440.11928629704762929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG222700.027.0668241
TACATGG226500.026.1950262
ACATGGG233400.024.4546093
CATGGGG142250.022.4738084
GAGTACT169500.021.35455312-13
TACCTGG40200.020.8171672
ATGGGGG82400.020.1970755
AGAGTAC281250.019.11535310-11
GTACTTT189300.018.83581414-15
AGTACTT180900.018.46369412-13
GTACCTG51450.017.7290651
CATGGGA91550.017.6139134
ACTTTTT227950.015.76576716-17
GAGTACA176150.015.4014881
GTATAGG15600.015.3714581
TACTTTT218550.015.07891514-15
GTATTAG18750.015.0459441
TGGGGGG84400.014.9733266
ACCTGGG54350.014.9654373
GTATTAT22450.014.8700171
Produced by FastQC (version 0.11.5)