FastQCFastQC Report
Mon 6 Jun 2016
ERR523107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737457
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT538700.9389177121501737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180640.3148433182157182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153760.26799329389309584No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC126790.2209864056497504No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC114000.19869429958255025No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA94270.16430624229514923No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG87170.1519314218825518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85420.1488812900907144No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA81640.14229300542034565No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC73150.1274955088988031No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG226050.026.189761
TACATGG232800.025.094082
ACATGGG232850.024.2188823
CATGGGG145150.022.2103864
TATCACG4800.021.5409362
GAGTACT163900.021.3116912-13
ATGGGGG82250.020.9117535
TACCTGG40600.019.3318582
AGAGTAC277750.019.15158710-11
GTACTTT180000.019.08915314-15
AGTACTT176450.018.4104512-13
CATGGGA89600.017.4132214
GTATAGG17600.016.5649191
GAGTACA176900.016.1616631
GTACCTG51350.015.84221651
TGGGGGG75100.015.7690626
TATACTG26350.015.6944945
ACTTTTT221250.015.63365716-17
AGTACAT175450.015.1080652
TACTTTT213600.014.87603714-15
Produced by FastQC (version 0.11.5)