FastQCFastQC Report
Mon 6 Jun 2016
ERR523106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6428483
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT607350.9447796626358038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218950.3405935739427171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175680.2732837591699317No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT175410.2728637533925189No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC138500.2154474080432351No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC124240.19326488068802547No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100380.15614881458036056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100260.15596214534595487No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG100070.1556665857248125No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89700.13953525271825407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG250550.027.2648161
TACATGG257600.026.18952
ACATGGG259900.025.0186713
CATGGGG168200.022.8924714
ATGGGGG94100.021.0843875
GAGTACT192450.020.71397612-13
GTACTTT205200.019.17656914-15
AGAGTAC315550.018.92310-11
AGTACTT203600.017.9873812-13
CATGGGA95850.016.3828374
GTGTAGC31150.016.3003561
ACTTTTT245800.016.15162716-17
GAGTACA199200.016.0963121
TATACCG4802.5465852E-1115.6717285
AGTACAT190100.015.654632
TACTTTT235350.015.46217814-15
GAGTACG9600.015.1817491
GTATAGG18550.014.6999361
TATACAG36450.014.3173825
TATCACG5007.712515E-1014.1040052
Produced by FastQC (version 0.11.5)