FastQCFastQC Report
Mon 6 Jun 2016
ERR523106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6428483
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT613060.9536620070396079No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199560.3104309368166642No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT172810.2688192533137289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168470.26206804933605643No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC142190.2211874870012101No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC129520.20147832700187587No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA103030.16027109350681956No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG99570.15488879724812216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95080.14790425672744253No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC80140.12466393704393401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG244000.026.3313331
TACATGG252000.025.2553252
ACATGGG254050.024.214053
CATGGGG161200.022.3894394
ATGGGGG88650.020.992145
GAGTACT189550.020.46238512-13
TATACCG5300.019.5068455
AGAGTAC308250.019.01885410-11
GTACTTT204100.018.76957914-15
GTATAGG19050.018.5173241
AGTACTT200400.018.50995612-13
TATAACG4950.018.041942
GAGTACA197850.016.9260621
CATGGGA95200.016.6356584
AGTACAT188000.016.3513932
GTATTAG19600.016.0779511
TACCTGG39850.016.0415152
ACTTTTT245700.015.77039716-17
TACTTTT232150.015.71205314-15
GTGTAGC35050.014.895221
Produced by FastQC (version 0.11.5)