FastQCFastQC Report
Mon 6 Jun 2016
ERR523105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7948849
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT597050.7511150356485574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277130.34864167126586504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216360.27219035108101813No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT177170.22288761555289324No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC137390.17284263419773102No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC124260.1563245194367134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121580.15295296212067935No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG100330.12621953190958843No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89460.11254459607925625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG302550.028.1428221
TACATGG308850.026.8211362
ACATGGG316700.025.5192933
CATGGGG219400.023.9770584
TACCTGG55850.023.4013272
ATGGGGG125550.022.4301265
GAGTACT223850.020.48448412-13
AGAGTAC363200.019.18874410-11
GTACTTT244650.018.50332314-15
GTACCTG73650.018.1935921
AGTACTT239500.017.50793612-13
CATGGGA105700.017.3012314
GAGTACA234100.017.0511071
AGTACAT229000.016.0747852
TGGGGGG118350.015.9993876
GTATAGG23050.015.5020441
ACTTTTT297950.015.40547916-17
ACCTGGG81600.015.2685063
TATCACG6550.015.0729022
TACTTTT279700.014.840814-15
Produced by FastQC (version 0.11.5)