FastQCFastQC Report
Mon 6 Jun 2016
ERR523105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7948849
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT606640.763179675447351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254750.32048665158943135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210770.26515788638078297No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT175940.22134022171008658No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC139810.17588710013235878No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC125550.15794739590599846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116150.14612178442438647No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA102290.12868529770788198No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89480.11256975695474905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG298650.027.8572181
TACATGG305500.026.7853662
TACCTGG59000.025.8905582
ACATGGG311200.025.5367073
CATGGGG213150.023.7019444
ATGGGGG119350.021.1843575
GTACCTG75000.021.0693171
GAGTACT230000.020.53093312-13
AGAGTAC372400.018.94774810-11
GTACTTT248550.018.86382114-15
ACCTGGG79900.018.5278343
AGTACTT244200.018.18205312-13
CATGGGA109750.016.7429944
GAGTACA234100.016.2524761
ACTTTTT297750.015.74449916-17
TACTTTT280050.015.53357414-15
AGTACAT227200.015.3085292
TGGGGGG108750.015.2978166
TATCACG7700.014.6497632
GTATAGA27450.014.5629381
Produced by FastQC (version 0.11.5)