FastQCFastQC Report
Mon 6 Jun 2016
ERR523104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304551
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT632680.6799683294766184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287170.3086339147369927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218390.23471309899854384No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT196920.21163836922383464No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC147540.15856756548489015No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC135910.14606830571405327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129190.13884603351628683No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG113790.12229499306307204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG347250.026.7401641
TACATGG351050.025.914442
ACATGGG360050.024.5496883
CATGGGG253950.021.7156374
GAGTACT253150.020.94427112-13
GTACTTT267450.019.526114-15
AGAGTAC412600.019.49927110-11
ATGGGGG139800.018.731835
AGTACTT263250.018.00795612-13
GAGTACA269450.017.3788221
AGTACAT261450.016.3817232
CATGGGA119500.016.051484
ACTTTTT331000.015.97528416-17
TATACTG40400.015.0120695
TACTTTT312050.014.9435914-15
GTATAGC28250.014.81191
GTATTAT32500.013.8875971
GTGTAAG42250.013.5760161
ATGGGGA103650.013.4262465
GTATTAC22250.013.3121991
Produced by FastQC (version 0.11.5)