FastQCFastQC Report
Mon 6 Jun 2016
ERR523104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304551
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT645720.6939829767175224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262160.28175459514381723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207350.22284793753078466No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT192730.20713519652909634No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC141700.1522910670273074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123350.13256953505870409No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG113690.1221875187744148No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA111240.11955439870231245No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA93200.10016603702854657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG345400.026.2938481
TACATGG350850.025.533662
ACATGGG361350.024.2031363
CATGGGG250350.022.1696054
GAGTACT251550.020.71750312-13
AGAGTAC413150.019.90810410-11
ATGGGGG140400.019.179455
GTACTTT267400.019.16066614-15
AGTACTT265750.018.60210212-13
GAGTACA271250.017.0095751
GTATAGG26850.016.4656071
AGTACAT267450.015.9219872
ACTTTTT327700.015.73945116-17
TACTTTT310600.015.64064214-15
TATACTG41800.015.1777065
CATGGGA116900.015.0353224
GTATAGC29200.014.6572581
GTGTAGC47950.013.7319941
TTATACT45850.013.3247684
ATGGGGA103700.013.3234665
Produced by FastQC (version 0.11.5)