FastQCFastQC Report
Mon 6 Jun 2016
ERR523103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4961903
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57650.030.7707211
GTATCAA83600.030.6188091
GTACATG175250.029.40031
TACATGG179350.028.3305192
ACATGGG188600.026.1899243
TCAACGC96950.025.7860244
CAACGCA99900.025.023315
CATGGGG146100.024.4124934
AACGCAG103200.024.3533466
ATCAACG103250.024.306613
TATCAAC106050.023.9338932
ATGGGGG79450.021.8238355
ACGCAGA122300.020.5115937
GAGTACA136700.019.1038171
CGCAGAG133350.018.7751648
AGTACAT131600.018.3585132
AGAGTAC116650.016.58925610-11
CGTATAC3401.9992513E-816.5872943
GCAGAGT154250.016.0172589
TGGGGGG61850.014.8867866
Produced by FastQC (version 0.11.5)