FastQCFastQC Report
Mon 6 Jun 2016
ERR523103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4961903
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88500.029.8781381
GTACATG173800.027.2067571
GGTATCA61000.026.8416941
TACATGG178650.025.9598962
TCAACGC98900.025.8964124
CAACGCA100450.025.54365
AACGCAG105050.024.5590346
ATCAACG105150.024.403573
ACATGGG188400.023.947513
TATCAAC110500.023.9223082
CATGGGG141900.022.9172784
ATGGGGG78400.020.4997925
ACGCAGA127100.020.2242477
GAGTACA136800.019.6042061
AGTACAT132500.018.9918372
CGCAGAG138250.018.4622028
TATACTG19600.017.2629835
GTATAGA15150.016.459771
AGAGTAC115000.016.4519310-11
GTATAGG12100.016.3314381
Produced by FastQC (version 0.11.5)