FastQCFastQC Report
Mon 6 Jun 2016
ERR523102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7555216
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT475570.6294591709886256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303130.40121950186467203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245630.32511314037877936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143470.18989529882401773No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT133610.1768447123153064No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC109000.14427118959934435No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC94540.1251320941717616No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG77800.10297521606265128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG288150.028.8479141
TACATGG293750.027.8001252
ACATGGG298850.026.5405373
ATGGGGG138950.025.613615
CATGGGG219100.025.2343794
GAGTACT246400.021.72562812-13
TACCTGG51900.020.4722632
AGAGTAC373100.020.19490210-11
TGGGGGG143750.019.4137336
AGTACTT256300.019.31927112-13
GTACTTT273300.019.21997614-15
GGGTTAG26800.018.2452281
GTACCTG66850.017.2311971
GAGTACA214850.016.5657581
TATAACG5200.016.2739872
CATGGGA91950.016.1059574
ACTTTTT332500.015.95338716-17
TACTTTT308700.015.82116714-15
AGTACAT217050.015.6170962
GTTAGAG32950.015.4104823
Produced by FastQC (version 0.11.5)