FastQCFastQC Report
Mon 6 Jun 2016
ERR523102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7555216
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT484360.6410935173792517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280640.37145198760697246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244270.32331305948102607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138230.18295969301208595No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT131400.1739195808564573No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110990.14690513150120393No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA84020.11120793899208176No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG79410.10510619418425628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG282850.027.2193031
TACATGG288050.026.385132
ACATGGG293650.025.1903573
CATGGGG212350.023.7026464
ATGGGGG137850.023.4890965
TACCTGG52000.022.145212
GAGTACT250200.021.5426312-13
GTACTTT274850.019.3938114-15
AGAGTAC379950.019.32071710-11
TGGGGGG132950.019.1232976
TATAACG5050.018.6146872
AGTACTT263800.018.42713212-13
GGGTTAG25750.018.2644791
GTACCTG70650.017.1748141
GAGTACA221450.016.544221
ACTTTTT325750.016.3536216-17
GTATAGG18950.015.8838321
CATGGGA96950.015.7541564
AGTACAT216700.015.681822
TATACTG32300.015.5680825
Produced by FastQC (version 0.11.5)