FastQCFastQC Report
Mon 6 Jun 2016
ERR523101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5498248
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148280.2696859072198999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119090.21659626848406985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76040.13829859984489604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.048.7696721
TACCTGG62300.045.7198682
GTATCAA133050.041.974371
GTACCTG75650.038.6216621
ACCTGGG75150.036.8957253
TCAACGC161350.034.017254
ATCAACG163750.033.434723
CAACGCA164950.033.217555
TATCAAC171350.032.1487352
AACGCAG172100.032.021056
GTACATG168500.030.29551
TACATGG172400.028.8448812
ACGCAGA197350.027.8767497
ACATGGG175500.027.6347543
CGCAGAG203400.026.9529748
CATGGGG125800.025.8867724
CCTGGGG73550.024.7259864
ATGGGGG81300.024.3339655
GCAGAGT228900.023.5178649
ATAACGC6100.021.5715453
Produced by FastQC (version 0.11.5)