FastQCFastQC Report
Mon 6 Jun 2016
ERR523101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5498248
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135310.24609657476345193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116490.21186748942572253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72810.13242400124548764No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78000.050.903821
TACCTGG62650.044.8914952
GTATCAA136550.039.757281
GTACCTG78550.036.2935141
ACCTGGG77100.035.779953
TCAACGC165850.032.189264
ATCAACG169250.031.5987263
CAACGCA169350.031.4962465
TATCAAC176350.030.4027842
AACGCAG178950.030.0686556
GTACATG164150.028.0572011
TACATGG169100.026.6443962
ACGCAGA205200.026.2669987
TATCACG5650.025.8046042
ACATGGG172550.025.3565833
CGCAGAG213250.025.2169028
CCTGGGG76000.025.1668534
CATGGGG123350.023.6973254
TATAACG4950.022.8028952
GCAGAGT238400.022.2411699
Produced by FastQC (version 0.11.5)