##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523100_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3026118
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754242894692144	33.0	31.0	34.0	30.0	34.0
2	32.019481394975344	34.0	31.0	34.0	30.0	35.0
3	32.089288983443474	34.0	31.0	34.0	28.0	36.0
4	34.23616891343959	35.0	34.0	37.0	32.0	37.0
5	34.37786233055023	35.0	34.0	37.0	32.0	37.0
6	34.77108724775438	35.0	35.0	37.0	32.0	37.0
7	34.68514016968274	36.0	34.0	37.0	32.0	37.0
8	34.892120862438276	36.0	35.0	37.0	32.0	37.0
9	35.931162631463806	37.0	35.0	39.0	32.0	39.0
10-11	35.938719012279094	37.0	34.5	39.0	32.0	39.0
12-13	36.1144613329685	37.5	35.5	39.0	32.0	39.0
14-15	36.6645833044184	38.0	36.0	40.0	32.0	41.0
16-17	36.91311112124511	38.0	36.5	40.0	32.0	41.0
18-19	36.8923477207432	38.0	35.5	41.0	32.5	41.0
20-21	37.03448427985954	38.5	36.0	41.0	32.0	41.0
22-23	36.90763265014782	38.5	36.0	40.5	31.5	41.0
24-25	36.85051789123887	38.0	36.0	40.5	32.0	41.0
26-27	36.63694789826438	38.0	36.5	40.0	31.5	41.0
28-29	36.564880153384635	38.0	35.5	40.0	31.5	41.0
30-31	36.47162751089019	38.0	36.0	40.0	30.5	41.0
32-33	36.29451660510264	37.5	35.5	40.0	30.5	41.0
34-35	36.0315176077073	37.5	35.0	40.0	30.0	41.0
36-37	35.954963752239664	37.5	35.0	40.0	30.0	41.0
38-39	35.80525808973741	37.0	35.0	40.0	30.0	41.0
40-41	35.96576918018398	37.5	35.0	40.0	30.0	41.0
42-43	35.70181070268906	37.0	35.5	40.0	29.5	41.0
44-45	35.51361017647032	38.0	35.0	40.0	28.0	41.0
46-47	35.14931902853755	37.0	35.0	40.0	27.5	41.0
48-49	34.899341995255966	37.0	34.0	40.0	26.0	41.0
50-51	33.88187506237364	36.5	33.0	38.5	25.0	40.0
52-53	34.317637316191906	37.0	34.0	38.5	26.5	39.5
54-55	35.28019016442849	38.0	35.0	40.0	27.5	41.0
56-57	35.24278729382	37.5	34.5	40.0	27.0	41.0
58-59	35.25645447401588	38.0	34.5	40.0	27.0	41.0
60-61	34.75890662558433	37.0	35.0	40.0	26.0	41.0
62-63	34.59310046733141	37.5	34.0	39.0	26.0	41.0
64-65	34.23083237335755	36.5	34.0	39.0	25.5	40.5
66-67	33.76142057249585	36.5	33.5	38.5	24.0	40.0
68-69	33.27731255027068	36.0	33.0	37.5	22.5	39.0
70-71	32.70796941824476	35.5	32.0	37.0	21.5	39.0
72-73	32.21096814466587	35.0	32.0	37.0	21.0	38.0
74-75	31.83826324683968	35.0	31.5	36.0	20.0	37.0
76-77	31.6413753528448	35.0	31.0	36.5	19.0	37.5
78-79	31.24356204880312	34.5	31.0	36.0	18.0	37.0
80-81	30.80852861653115	34.0	30.0	35.5	18.0	37.5
82-83	30.49858515100865	34.0	29.5	35.0	16.5	37.0
84-85	30.198961342551744	34.0	29.0	35.0	17.0	36.5
86-87	30.15234799171744	34.0	29.0	35.0	15.0	37.0
88-89	29.836256715699783	34.0	29.0	35.0	10.5	36.5
90-91	29.479232468793352	34.0	28.5	35.0	10.0	36.0
92-93	29.069463252920077	34.0	26.0	35.0	10.0	35.0
94-95	28.906370967688638	33.5	25.5	35.0	10.0	36.0
96-97	28.658789082249932	33.5	25.0	35.0	9.5	35.0
98-99	28.651308375945682	33.5	25.0	35.0	9.5	36.0
100	27.09840230949355	32.0	23.0	34.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.021193321449175073
1101	2	-0.07632128889433432
1101	3	-0.045796408957450296
1101	4	0.004150074342888388
1101	5	-0.09152099152977655
1101	6	-0.04697030850211803
1101	7	0.07089024904900754
1101	8	4.529437136326919E-4
1101	9	0.01616857744343747
1101	10-11	0.006704879277421583
1101	12-13	0.022202710085281296
1101	14-15	-0.13043388933456868
1101	16-17	0.034204742117807996
1101	18-19	-0.0030645727056537453
1101	20-21	0.05828883651476957
1101	22-23	0.03305711048570714
1101	24-25	-0.07117664503962118
1101	26-27	-0.08851934014739982
1101	28-29	-0.039491987154711694
1101	30-31	-0.02497877748253785
1101	32-33	-0.06363378338554782
1101	34-35	-0.16012231203045957
1101	36-37	-0.09078926925744923
1101	38-39	-0.18429150988445286
1101	40-41	-0.3091908240188985
1101	42-43	-0.2799455082271791
1101	44-45	-0.16471306443351352
1101	46-47	-0.31298204502108007
1101	48-49	-0.2861229004487811
1101	50-51	-0.2698213417608315
1101	52-53	-0.20036496313382912
1101	54-55	-0.13687413841100948
1101	56-57	-0.16356474574701707
1101	58-59	-0.22731575497067524
1101	60-61	-0.22915352932238875
1101	62-63	-0.037340938269302626
1101	64-65	-0.17139954049150674
1101	66-67	-0.18899255560863537
1101	68-69	-0.19419266593158824
1101	70-71	-0.30647827211424783
1101	72-73	-0.2414851392226396
1101	74-75	-0.2533573332540229
1101	76-77	-0.2720929845180571
1101	78-79	-0.3404018896200114
1101	80-81	-0.20642358452334264
1101	82-83	-0.3584105196557559
1101	84-85	-0.39182493203988145
1101	86-87	-0.3186639817296246
1101	88-89	-0.39251979352159694
1101	90-91	-0.4431621391414424
1101	92-93	-0.39793364677304766
1101	94-95	-0.4372384110094032
1101	96-97	-0.4455915014239906
1101	98-99	-0.4886297084346971
1101	100	-0.4111254663941857
1102	1	-0.19669722930602163
1102	2	-0.3024603747220027
1102	3	-0.3228295833954711
1102	4	-0.22862510895132004
1102	5	-0.231623103950227
1102	6	-0.20224893990531712
1102	7	-0.18606646205995503
1102	8	-0.18333126515708642
1102	9	-0.17926540034348193
1102	10-11	-0.18778056607308002
1102	12-13	-0.2692707818207367
1102	14-15	-0.35925403113049015
1102	16-17	-0.278652469874352
1102	18-19	-0.1813469304862778
1102	20-21	-0.30153585436528374
1102	22-23	-0.23051755014778763
1102	24-25	-0.432090817372476
1102	26-27	-0.3316751462789824
1102	28-29	-0.2964928582559381
1102	30-31	-0.453088124882548
1102	32-33	-0.3505636355403112
1102	34-35	-0.42259418272032434
1102	36-37	-0.7531794742082027
1102	38-39	-0.4912270483943857
1102	40-41	-1.0681667961436574
1102	42-43	-1.0762802288698623
1102	44-45	-0.4940970022949571
1102	46-47	-0.34816364542759715
1102	48-49	-0.5306767030475683
1102	50-51	-0.543278766688843
1102	52-53	-0.3382935570312853
1102	54-55	-0.2731476399959263
1102	56-57	-0.18341738445778333
1102	58-59	-0.30525451171565976
1102	60-61	-0.2740696858302343
1102	62-63	-0.5498497097746124
1102	64-65	-0.27209460667100416
1102	66-67	-0.21568454873661835
1102	68-69	-0.15214206946922815
1102	70-71	-0.4762262576926801
1102	72-73	-0.2689798763194524
1102	74-75	-0.23154532173612807
1102	76-77	-0.4042190764192348
1102	78-79	-0.3337071138075842
1102	80-81	-0.5211494296125139
1102	82-83	-0.6872215874806642
1102	84-85	-0.5740169614637445
1102	86-87	-0.5863207194555677
1102	88-89	-0.5939020617723756
1102	90-91	-0.7202648806905501
1102	92-93	-0.8491053988963557
1102	94-95	-0.8579276698474487
1102	96-97	-0.862172671119346
1102	98-99	-0.7784196762523479
1102	100	-0.881775404691183
1103	1	-0.09014604951207161
1103	2	-0.16048825187485605
1103	3	-0.09540181767302869
1103	4	-0.04944964514665884
1103	5	-0.12601883831575833
1103	6	-0.02673552915717181
1103	7	-0.12999326009721557
1103	8	-0.11525070292221784
1103	9	-0.14559557648173183
1103	10-11	-0.14507522621030944
1103	12-13	-0.11972249042042904
1103	14-15	-0.1872120687822445
1103	16-17	-0.1825160018275156
1103	18-19	-0.231906281843969
1103	20-21	-0.32371429453497313
1103	22-23	-0.23837107291281967
1103	24-25	-0.15753287355686751
1103	26-27	-0.1799368562471244
1103	28-29	-0.35511686369060413
1103	30-31	-0.23814252051916895
1103	32-33	-0.37781264830593386
1103	34-35	-0.31963003594496087
1103	36-37	-0.43716856086978595
1103	38-39	-0.43401816035262897
1103	40-41	-0.3559382855968707
1103	42-43	-0.37579760810470475
1103	44-45	-0.41058467163986734
1103	46-47	-0.34070220621581626
1103	48-49	-0.4501378050357161
1103	50-51	-0.5053301126509666
1103	52-53	-0.31004973457707763
1103	54-55	-0.3210166554984397
1103	56-57	-0.21346498674895287
1103	58-59	-0.2804018637771293
1103	60-61	-0.3266980751720183
1103	62-63	-0.3037579605125913
1103	64-65	-0.39919797792977363
1103	66-67	-0.38143704614634544
1103	68-69	-0.25165187339079154
1103	70-71	-0.30763167379731016
1103	72-73	-0.33099933265632586
1103	74-75	-0.358136381889004
1103	76-77	-0.39759153258016156
1103	78-79	-0.4082543055656487
1103	80-81	-0.345246378960546
1103	82-83	-0.5074289800089602
1103	84-85	-0.32600596992854847
1103	86-87	-1.0801896030010099
1103	88-89	-0.608058245182928
1103	90-91	-0.8809310439654325
1103	92-93	-0.4892022651603085
1103	94-95	-0.7573870124080031
1103	96-97	-0.6106721481783666
1103	98-99	-0.750402257919113
1103	100	-0.748425454541632
1104	1	-0.08723413658971069
1104	2	-0.15548997339542936
1104	3	-0.08995066190671963
1104	4	-0.009079009592561249
1104	5	0.024717894238193594
1104	6	-0.0377268511284683
1104	7	-0.10746775029237199
1104	8	0.00934239221526667
1104	9	-0.07485770928921909
1104	10-11	-0.1609401142332274
1104	12-13	-0.11078476642712332
1104	14-15	-0.2023536247238411
1104	16-17	-0.17052062254523292
1104	18-19	-0.09167962801046059
1104	20-21	-0.18986460284365592
1104	22-23	-0.15178201161104
1104	24-25	-0.1880184597346215
1104	26-27	-0.12751019693604349
1104	28-29	-0.05824210822478193
1104	30-31	-0.06993039788429911
1104	32-33	-0.046578885374970014
1104	34-35	-0.16298731406443778
1104	36-37	-0.1771998808065618
1104	38-39	-0.061637121336708844
1104	40-41	0.023353778193296648
1104	42-43	-0.16240632995393156
1104	44-45	-0.09102594822562793
1104	46-47	0.0762158415798524
1104	48-49	0.035895870254201157
1104	50-51	-0.19573517861650203
1104	52-53	-0.07081950858837871
1104	54-55	0.05166173707085164
1104	56-57	0.03166291440447111
1104	58-59	-0.04125624661200078
1104	60-61	-0.12259322674407969
1104	62-63	-0.08928241784236235
1104	64-65	-0.17308749934706213
1104	66-67	-0.0439084845550326
1104	68-69	-0.0493780968441726
1104	70-71	-0.0771616399929016
1104	72-73	-0.12029255064778255
1104	74-75	-0.08014602240420743
1104	76-77	-0.06765673517183401
1104	78-79	-0.07493857869270926
1104	80-81	-0.1397178525821552
1104	82-83	-0.186658345237376
1104	84-85	-0.2655781525036325
1104	86-87	-0.10756960519203673
1104	88-89	-0.27776740601990113
1104	90-91	-0.3805715787323223
1104	92-93	-0.15954281322985864
1104	94-95	-0.4612478157321007
1104	96-97	-0.3543489835540541
1104	98-99	-0.3932904216826678
1104	100	-0.1759337882662173
1105	1	-0.1007496058727142
1105	2	-0.0726484130975642
1105	3	0.01096762347029312
1105	4	-0.09492165039254985
1105	5	-0.08850100266908356
1105	6	0.0018507819118909197
1105	7	-0.04135370169052521
1105	8	-0.05657719428995733
1105	9	-0.047102664588869914
1105	10-11	-0.07286068789876765
1105	12-13	0.047276972106445214
1105	14-15	0.021589750742165847
1105	16-17	0.03420164786692936
1105	18-19	0.008401173937080841
1105	20-21	-0.09958139060324811
1105	22-23	-0.09754193648502252
1105	24-25	-0.07577470185007229
1105	26-27	-0.028605360321918738
1105	28-29	0.01830088369125349
1105	30-31	-0.2522468633789501
1105	32-33	-0.13129576741921767
1105	34-35	-0.1475333523176019
1105	36-37	-0.0711840956699703
1105	38-39	-0.15706674350039407
1105	40-41	-0.20379445048092748
1105	42-43	-0.12714056980276922
1105	44-45	-0.23972698856248087
1105	46-47	-0.23485221025407554
1105	48-49	-0.10905594085969739
1105	50-51	-0.2592219853650164
1105	52-53	-0.14570037992942986
1105	54-55	0.022567968154994844
1105	56-57	-0.01855886667033957
1105	58-59	-0.005134617524049645
1105	60-61	-0.1086974367424034
1105	62-63	0.02219244869771586
1105	64-65	0.0773333637723681
1105	66-67	-0.0011313018181766665
1105	68-69	0.05504769547691524
1105	70-71	0.02480615142681586
1105	72-73	-0.05652783988481502
1105	74-75	0.007975360600795511
1105	76-77	0.06180917526905816
1105	78-79	0.09778890188936629
1105	80-81	-0.07570540889366484
1105	82-83	-0.07065911177160444
1105	84-85	-0.13613769891902194
1105	86-87	-0.14918567242830605
1105	88-89	-0.11344775936106544
1105	90-91	-0.14753586276295394
1105	92-93	-0.026423961767786608
1105	94-95	-0.05369827857546383
1105	96-97	-0.18697710696888947
1105	98-99	-0.1926738943372932
1105	100	-0.12796128544239238
1106	1	-0.11255331463596008
1106	2	-0.1302232732428692
1106	3	0.08607003312549466
1106	4	0.04213894609360125
1106	5	-0.08897171401432757
1106	6	-0.038383293352183045
1106	7	0.06976181050514185
1106	8	-1.2180302645530716
1106	9	-0.024485491086139177
1106	10-11	-0.00882333405187552
1106	12-13	0.07042772593547397
1106	14-15	-0.05774399465551028
1106	16-17	-0.10811714438541031
1106	18-19	-0.02070843087725649
1106	20-21	-0.010506407483454439
1106	22-23	0.043226778436157076
1106	24-25	0.10831108644404708
1106	26-27	0.08942217030426036
1106	28-29	-0.03389119417232678
1106	30-31	0.045570869081991816
1106	32-33	0.09735157766746738
1106	34-35	0.051670698747663835
1106	36-37	0.06476397316995275
1106	38-39	0.04729175799786134
1106	40-41	-0.026481010152110684
1106	42-43	0.0017515497892262033
1106	44-45	-0.0555935303816355
1106	46-47	0.010915302556917084
1106	48-49	-0.09330610966176067
1106	50-51	-0.0623227848373773
1106	52-53	-0.13441896731638536
1106	54-55	-0.04315199451971097
1106	56-57	-0.018164611375290463
1106	58-59	0.03165663258764084
1106	60-61	0.0032265060684721902
1106	62-63	-0.05539950352001455
1106	64-65	-0.15462957162411328
1106	66-67	-0.11823732977898516
1106	68-69	-0.13719540067992142
1106	70-71	-0.158382660013217
1106	72-73	-0.11606948586381804
1106	74-75	0.022528147976519364
1106	76-77	-0.05010451966761664
1106	78-79	-0.024565189108074037
1106	80-81	-0.14511932721097054
1106	82-83	-0.17019832589552308
1106	84-85	-0.14267469121169896
1106	86-87	-0.18608262533230047
1106	88-89	-0.176242126668825
1106	90-91	-0.24618857819629625
1106	92-93	-0.34320981850388677
1106	94-95	-0.2033866741130126
1106	96-97	-0.14942866561298374
1106	98-99	-0.011626197737005128
1106	100	-0.1218056522435198
1107	1	-0.07369210558199057
1107	2	-0.16445182817274784
1107	3	-0.09492806730585812
1107	4	0.1006224068719419
1107	5	-0.07235939687864601
1107	6	0.07221678394628128
1107	7	0.09625937688268493
1107	8	0.12426670591361955
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2316	74-75	-0.2651904877233129
2316	76-77	-0.25729429311045493
2316	78-79	-0.29141860186815904
2316	80-81	-0.07025879015929348
2316	82-83	-0.04092086203614542
2316	84-85	0.1203243357715138
2316	86-87	0.10882770974721367
2316	88-89	0.24011171482154836
2316	90-91	0.30484100251887725
2316	92-93	0.3911161957111986
2316	94-95	0.41727264105734463
2316	96-97	0.5686093657781122
2316	98-99	0.49840207166638706
2316	100	0.3120765172657194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	5146.0
1	518.0
2	318.0
3	226.0
4	203.0
5	180.0
6	233.0
7	618.0
8	1614.0
9	3234.0
10	5093.0
11	6276.0
12	6055.0
13	5510.0
14	5373.0
15	6429.0
16	8811.0
17	13028.0
18	16132.0
19	20130.0
20	25384.0
21	29747.0
22	28743.0
23	27412.0
24	28285.0
25	30830.0
26	35224.0
27	42443.0
28	51765.0
29	64553.0
30	80752.0
31	105057.0
32	137441.0
33	188964.0
34	274849.0
35	452583.0
36	482228.0
37	506001.0
38	316129.0
39	12591.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.927823983924405	16.86626574763281	12.8842012043456	16.321709064097185
2	13.006049194471366	28.135450634069205	28.615834650280636	30.242665521178797
3	33.93470981162557	24.3895389594312	29.432987418392237	12.242763810550997
4	13.116757985434294	17.09533069480595	38.89831483149144	30.889596488268317
5	9.339386528932932	43.214201785929134	33.960429283245205	13.485982401892723
6	27.054169162351123	32.78477968185987	14.406183908533384	25.754867247255632
7	20.5071995393673	35.48793307308999	23.333345480820167	20.67152190672254
8	25.627988548356427	20.690842064184594	20.87635816002486	32.80481122743412
9	20.32709606902262	24.27576578137647	27.234294495134115	28.1628436544668
10-11	26.61616883505133	23.12521665765945	23.235688255444785	27.02292625184444
12-13	35.67276579145482	22.63426535954966	22.091414818612254	19.601554030383266
14-15	27.72195100948545	24.845149303163584	30.815768904668538	16.617130782682423
16-17	19.109678765606706	28.253340774758335	32.1138670513243	20.52311340831066
18-19	19.79568357620988	34.078951247685715	27.544191318986773	18.581173857117633
20-21	23.385389682235758	24.844023087961904	24.204198076268188	27.566389153534153
22-23	18.93286218261959	26.931692255509827	26.2454365969563	27.890008964914276
24-25	28.28144193662664	27.27330392716881	21.24865621071212	23.19659792549242
26-27	25.24389484465326	19.669789633657555	33.00990684098043	22.076408680708756
28-29	19.446460133823372	32.905039138789284	26.821851467884727	20.82664925950262
30-31	23.546793118336126	24.79048147898505	25.475680118524423	26.187045284154397
32-33	21.5151966873706	26.700306418219462	29.927469979296067	21.857026915113874
34-35	24.11915659486155	25.005440116161797	27.907961514780062	22.967441774196594
36-37	23.198744216196737	22.86952826291963	32.89392051847786	21.03780700240577
38-39	23.492066594136517	26.22776376547732	29.16311482591191	21.117054814474255
40-41	20.7889260012894	29.64210533987884	26.661477482873707	22.907491175958057
42-43	21.250295021527254	28.686129392094166	22.06376771267843	27.999807873700146
44-45	25.597612926602594	21.484803239997056	25.337085685177367	27.580498148222976
46-47	27.117037402107808	28.29504754816795	28.665393813562957	15.922521236161282
48-49	24.39695476752916	25.548547780791885	29.67905878487162	20.375438666807334
50-51	14.522698501340466	28.149798383490698	35.761939493186375	21.56556362198246
52-53	20.12605190949545	31.80389952287045	22.735572570367747	25.334475997266352
54-55	17.786751339214867	25.43098550112925	26.213642250311675	30.568620909344208
56-57	24.843050768396935	21.377744251102868	25.57320012774591	28.20600485275429
58-59	30.380215036664893	21.252304496687984	25.854423722609	22.513056744038124
60-61	23.614508192825394	25.412042619484946	32.09696948017073	18.87647970751892
62-63	17.418753994340584	26.407871497280123	31.960953281165466	24.212421227213827
64-65	16.097282689988326	33.433052347678796	27.294402831357424	23.17526213097546
66-67	22.38799789022826	25.08199882319265	23.06184599726472	29.468157289314377
68-69	20.443176786148264	24.29189141012407	26.5379095599029	28.727022243824763
70-71	26.089329546234058	25.257214891372747	23.697107614528438	24.956347947864753
72-73	24.033930020203307	19.65547000524968	33.340790262112705	22.969809712434312
74-75	19.924444675655856	30.559564017745995	25.936911536120395	23.579079770477758
76-77	20.33117120651448	23.74490393689099	27.973725122729636	27.950199733864896
78-79	22.11165740970528	24.490795334398687	30.094821590748317	23.302725665147715
80-81	24.340943458515245	22.935593351732095	27.817338443376105	24.906124746376555
82-83	20.472366983711723	21.963263990288194	31.881797757127384	25.682571268872696
84-85	20.33002927411818	26.24804351834839	28.651258253589845	24.770668953943588
86-87	20.998776206079807	25.904687211891957	27.17849648128185	25.91804010074639
88-89	19.966312878994607	25.323380290184428	24.844467132275298	29.865839698545667
90-91	23.319176431118677	21.20329526957343	26.553742798328344	28.923785500979555
92-93	24.803707620714242	25.043980789409826	27.97507824282232	22.17723334705361
94-95	21.502351602515887	23.47835047857116	29.29638806842544	25.722909850487518
96-97	17.749670020280064	24.40320763316361	32.89026780585811	24.956854540698217
98-99	23.031720080107736	26.048465512757442	24.793957796010186	26.125856611124636
100	21.485593703638777	18.0668466466495	26.841086202502996	33.60647344720872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6160.0
1	4882.5
2	3137.5
3	2139.5
4	1362.0
5	1212.5
6	1681.0
7	2534.0
8	3615.5
9	4281.0
10	3425.0
11	2367.0
12	2178.5
13	2112.0
14	2169.5
15	2251.5
16	2270.0
17	2328.5
18	2527.5
19	2746.5
20	3016.5
21	3491.5
22	4094.5
23	4841.0
24	5815.5
25	7018.0
26	8404.0
27	9858.0
28	11458.5
29	13211.5
30	14546.5
31	15437.0
32	16196.0
33	16214.0
34	16501.0
35	16566.5
36	15560.5
37	14505.5
38	13203.5
39	12733.5
40	12338.5
41	11654.0
42	11466.5
43	11900.5
44	12934.0
45	19818.0
46	68580.0
47	261697.5
48	604367.0
49	665395.5
50	499014.0
51	321829.0
52	118895.0
53	42955.0
54	22134.0
55	13235.5
56	8791.0
57	6767.5
58	5770.0
59	4756.0
60	4239.0
61	4092.5
62	3609.0
63	2732.0
64	1999.5
65	1569.5
66	1481.5
67	1273.0
68	952.5
69	772.5
70	672.0
71	584.0
72	516.5
73	446.0
74	371.5
75	310.0
76	260.5
77	211.5
78	177.0
79	138.0
80	102.5
81	85.5
82	71.5
83	49.5
84	35.0
85	29.5
86	21.5
87	16.5
88	9.0
89	3.0
90	3.5
91	3.0
92	1.5
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1452025334107923
2	0.0687018814203544
3	0.30055007769029496
4	0.2984351568577299
5	0.30035180386224203
6	0.25028766227886684
7	0.25316263278563494
8	0.2482388327223195
9	0.25977176038740063
10-11	0.23933303327894023
12-13	0.2483544924553504
14-15	0.2233224216636628
16-17	0.23075769021564924
18-19	0.24351330648705702
20-21	0.24460381254134836
22-23	0.2354997392699161
24-25	0.23583019565000438
26-27	0.23773031983551204
28-29	0.24577693269066175
30-31	0.23110466941474195
32-33	0.24348026084904817
34-35	0.22738703513874872
36-37	0.2355162620889205
38-39	0.2360615151160662
40-41	0.2531295871476261
42-43	0.2402583111431874
44-45	0.22656089418852798
46-47	0.22856015528806214
48-49	0.23692070170429572
50-51	0.23198037882197586
52-53	0.24589259242369269
54-55	0.25593846637837653
56-57	0.25200603545532596
58-59	0.24992416026076975
60-61	0.23074116739664483
62-63	0.2572602918987297
64-65	0.2523530146544187
66-67	0.25716115498470316
68-69	0.26286152754122605
70-71	0.2810531512650862
72-73	0.29047115809760227
74-75	0.27087509475836696
76-77	0.2677357591475283
78-79	0.2653234275728838
80-81	0.2517251475322509
82-83	0.26221713760005394
84-85	0.2731056753239629
86-87	0.2658356349620207
88-89	0.27584846327869567
90-91	0.2695037007810006
92-93	0.2468674387449531
94-95	0.2701811363601816
96-97	0.2686445141927711
98-99	0.25388963682182913
100	0.27675721832393846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3026118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.229311472920042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82350470660748	7.960808695581467
2	8.294162476605214	1.6968714276040124
3	3.5481443139720943	1.0888522001547227
4	1.9826800347840137	0.8112580650778247
5	1.3484330853960578	0.6896771015453432
6	0.9433925736911422	0.579015388651582
7	0.753473961641068	0.5395263888253043
8	0.5394131946945866	0.44142604649070294
9	0.4691646120376487	0.4319307853744321
>10	3.5788816695900465	7.178945954085221
>50	0.3655428460495844	2.6097385086249414
>100	0.272046937400995	5.67569149945464
>500	0.03473630346289237	2.463920652110541
>1k	0.024347876259036713	4.895728909070523
>5k	0.008765235453253216	6.677584944844625
>10k+	0.01331017235494007	56.25902343250411
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	225062	7.437317381542953	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	142329	4.703352612158548	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	93738	3.0976320156715635	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	73904	2.4422048314044593	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	63572	2.1007772994972433	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	62640	2.0699787648730155	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	62420	2.062708724511073	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	61024	2.016577013850749	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	58329	1.927519019416956	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	58069	1.9189271535346604	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	55602	1.8374035645668807	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	50815	1.679214095418619	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	50095	1.6554212360522624	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	43277	1.4301160761080698	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	39895	1.3183557283622118	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	38828	1.283096032606792	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	37964	1.2545446013671642	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	36973	1.221796374100415	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	32275	1.066547966734939	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	27692	0.915099807740478	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	27277	0.9013858679668143	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	27099	0.8955037444012428	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	25205	0.8329153060125216	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	24367	0.8052230613611233	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	24099	0.7963668303747574	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	23553	0.778323912021937	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	22551	0.7452121827370909	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	21530	0.711472586330077	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	20843	0.6887702330180119	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	19814	0.6547662715069273	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	19631	0.6487189197513118	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	19173	0.6335840175432683	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	12985	0.42909760954463777	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	12774	0.42212497992477493	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	12240	0.4044786092280605	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11707	0.38686528416935495	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	11491	0.379727426359448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11353	0.37516712831422966	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	11200	0.3701111456988789	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	10698	0.353522235418447	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	10034	0.33157993178058487	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	9995	0.3302911518982406	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9528	0.31485883894811767	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	9399	0.31059595164497883	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	9378	0.3099019932467934	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9125	0.3015414468305598	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	9033	0.2985012481337476	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	8894	0.29390790445052045	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	8795	0.29063638628764643	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	8583	0.2836307110297748	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	8393	0.27735203980809736	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	7900	0.26106054026974496	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	7685	0.2539557280978468	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	7666	0.25332786097567905	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	7601	0.2511798945051052	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	7267	0.2401426514101565	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	6869	0.2269904874826428	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	6859	0.22666003110255448	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	6797	0.22461120154600714	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	6706	0.22160404848720372	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6294	0.20798924562756643	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5891	0.19467185351000854	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	5787	0.19123510715709038	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5772	0.19073942258695795	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	5660	0.18703831112996916	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	5142	0.16992067064139602	No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC	5042	0.16661610684051315	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	5021	0.16592214844232775	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	4698	0.15524840736547615	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	4642	0.15339785163698177	No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT	4269	0.14107182865968876	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	4189	0.13842817761898246	No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG	4057	0.13406615340181713	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	3905	0.1290432164244752	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	3685	0.12177317606253293	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	3585	0.11846861226165008	No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG	3539	0.11694851291324396	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3508	0.11592409813497029	No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA	3276	0.10825751011692207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.3045638008828473E-5	0.0
9	0.0	0.0	0.0	9.913691402648542E-5	0.0
10-11	0.0	0.0	0.0	1.4870537103972814E-4	0.0
12-13	0.0	0.0	0.0	2.4784228506621354E-4	0.0
14-15	0.0	0.0	0.0	5.122073891368413E-4	0.0
16-17	0.0	0.0	0.0	7.104812171898121E-4	0.0
18-19	0.0	0.0	0.0	0.0010905060542913394	0.0
20-21	0.0	0.0	0.0	0.0038332940090241028	0.0
22-23	0.0	0.0	0.0	0.012309500158288605	0.0
24-25	0.0	0.0	0.0	0.02375981372834767	0.0
26-27	0.0	0.0	0.0	0.043273262972560886	0.0
28-29	0.0	0.0	0.0	0.11769203976844261	0.0
30-31	0.0	0.0	0.0	0.26096140335571844	0.0
32-33	0.0	0.0	0.0	0.5587356474532719	0.0
34-35	0.0	0.0	0.0	1.0006880101833437	0.0
36-37	0.0	0.0	0.0	1.527336343130043	0.0
38-39	0.0	0.0	0.0	2.212835057985181	0.0
40-41	0.0	0.0	0.0	3.050475890232965	0.0
42-43	0.0	0.0	0.0	3.7621137047530864	0.0
44-45	0.0	0.0	0.0	4.475866440105772	0.0
46-47	0.0	0.0	0.0	5.3697344254255786	0.0
48-49	0.0	0.0	0.0	6.07400306266973	0.0
50-51	0.0	0.0	0.0	6.8288645717054	0.0
52-53	0.0	0.0	0.0	7.6049248575237325	0.0
54-55	0.0	0.0	0.0	8.445192817993217	0.0
56-57	0.0	0.0	0.0	9.538953867628427	0.0
58-59	0.0	0.0	0.0	10.357543889564122	0.0
60-61	0.0	0.0	0.0	11.145071011771517	0.0
62-63	0.0	0.0	0.0	11.90953227864875	0.0
64-65	0.0	0.0	0.0	12.731625138213381	0.0
66-67	0.0	0.0	0.0	13.684562201473966	0.0
68-69	0.0	0.0	0.0	14.859235495773792	0.0
70-71	0.0	0.0	0.0	16.157235111122567	0.0
72-73	0.0	0.0	0.0	17.117111758365006	0.0
74-75	0.0	0.0	0.0	18.21559833423548	0.0
76-77	0.0	0.0	0.0	19.169163264618234	0.0
78-79	0.0	0.0	0.0	20.62363397593881	0.0
80-81	0.0	1.6522819004414237E-5	0.0	21.941510542549892	0.0
82-83	0.0	3.3045638008828473E-5	0.0	23.13640776731112	0.0
84-85	0.0	3.3045638008828473E-5	0.0	24.396900583519876	0.0
86-87	0.0	3.3045638008828473E-5	0.0	25.493007873453713	0.0
88	0.0	3.3045638008828473E-5	0.0	26.5151920711618	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCA	1255	0.0	52.1015	1
TACCCAT	1440	0.0	45.064037	2
ACCCATG	1530	0.0	42.41321	3
CGCCGTT	125	6.2682375E-9	33.843895	94
CTATTGG	60	0.004144198	31.360853	1
GCTTATG	1380	0.0	29.315584	1
GTATCAT	690	0.0	28.611992	94
GCCGGAT	280	0.0	28.539001	94
GGGTCGC	275	0.0	27.348602	94
CGGATCA	155	5.0835297E-8	27.293467	94
TCGTCGG	70	0.008833292	26.860237	94
GGTAAGT	35	0.008838265	26.857565	50-51
CGTCTCA	125	8.022389E-6	26.323029	94
CTTATGT	1545	0.0	26.174805	2
TAGAGTG	90	9.5075124E-4	26.117584	5
GCTTTGC	705	0.0	24.688334	1
GAGTAGG	680	0.0	24.212425	1
GCCGCAT	205	2.9867806E-8	22.92947	94
TATGTAC	1785	0.0	22.652872	4
TTATGTA	1765	0.0	22.645803	3
>>END_MODULE