FastQCFastQC Report
Mon 6 Jun 2016
ERR523099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7356310
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245590.3338494435389482No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT196140.26662824160482634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196090.266560272745439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126640.17215152705636386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82450.11208064912979471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG110950.053.1082882
ACCTGGG120050.047.6332243
GTACCTG156700.038.231751
TATAACG6800.035.96112
CCTGGGG111000.034.0618824
TAACGCA8900.031.7027424
ATAACGC10000.027.745373
GTACATG260700.027.6338561
TACATGG262900.026.6522712
CATGGGG161250.026.3052464
ACATGGG262400.025.878673
ATGGGGG107300.025.067515
CTGGGGG74750.024.1565515
TGGGGGG124600.022.8237386
TACCGGG12400.022.3753012
GTACCGG15550.020.2613411
CGCCGTA32600.019.74796994
GAGTACT217900.019.72599612-13
CGTATCA16850.019.24281794
GTATCAA432500.018.7771641
Produced by FastQC (version 0.11.5)