FastQCFastQC Report
Mon 6 Jun 2016
ERR523099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7356310
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235240.31977988964575993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198530.26987715308354326No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT195700.26603011564221735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128590.1748023125724718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG115250.053.142962
ACCTGGG127700.047.0048033
GTACCTG159950.038.8145681
TATAACG6800.035.944852
CCTGGGG112750.035.3109254
TAACGCA8550.029.6872444
ATAACGC9750.028.9260313
GTACATG250650.027.0771961
CTGGGGG74850.026.8152125
CATGGGG153350.026.207444
TACATGG253900.026.1590332
ACATGGG249600.025.4609363
ATGGGGG100900.025.3892885
TGGGGGG112350.024.8487996
TACCGGG9600.024.4816672
TATCACG5950.022.119912
GTACCGG14800.020.974291
GTATAAC19150.020.1395661
GAGTACT227700.018.25658212-13
AGTACTT236000.017.74395212-13
Produced by FastQC (version 0.11.5)