FastQCFastQC Report
Mon 6 Jun 2016
ERR523098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6191492
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205420.3317778654967171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164690.26599404473106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101500.1639346380484704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111150.044.2301331
TACCTGG70200.043.253012
GTATCAA188850.038.5874331
GTACCTG86300.035.7605741
ACCTGGG86850.033.874493
TCAACGC226100.031.654024
ATCAACG229250.031.242363
CAACGCA231000.031.0021615
TATCAAC234150.030.7729362
AACGCAG239350.030.1891216
GTACATG206100.029.902311
TACATGG212650.028.4689462
ACATGGG216400.027.081793
ACGCAGA270800.026.6132097
CGCAGAG279750.025.7263158
CATGGGG154050.025.5324954
CCTGGGG83100.024.5990164
ATGGGGG101750.024.384765
TATAACG6300.023.8742852
GCAGAGT306350.023.1230059
Produced by FastQC (version 0.11.5)