FastQCFastQC Report
Mon 6 Jun 2016
ERR523098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6191492
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183030.2956153379508526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156420.2526370057491797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92790.14986694644844892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112350.045.8927151
TACCTGG72600.043.1402242
GTATCAA186150.037.4534151
ACCTGGG85200.035.852863
GTACCTG89000.035.785321
TCAACGC222550.030.6385944
ATCAACG226300.030.153113
CAACGCA228250.029.8528795
GTACATG203550.029.5831991
TATCAAC234500.029.4593892
AACGCAG236800.029.1319716
TACATGG209200.028.009232
ACATGGG210400.027.2275163
ACGCAGA267000.025.8016957
CATGGGG153350.025.4345074
CGCAGAG276850.024.8905338
CCTGGGG81450.024.5780564
TATAACG5400.024.384232
ATGGGGG97950.023.3163625
ATAACGC6450.023.3152643
Produced by FastQC (version 0.11.5)