##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523097_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	944757
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558732033739894	33.0	31.0	34.0	30.0	34.0
2	31.821301138811357	34.0	31.0	34.0	28.0	35.0
3	31.814051655610914	34.0	31.0	34.0	28.0	36.0
4	34.02963195827075	35.0	34.0	37.0	32.0	37.0
5	34.142385819845735	35.0	34.0	37.0	31.0	37.0
6	34.53016278259912	35.0	35.0	37.0	32.0	37.0
7	34.439991447536244	35.0	34.0	37.0	32.0	37.0
8	34.64657790310101	36.0	35.0	37.0	32.0	37.0
9	35.69073105571062	37.0	35.0	39.0	32.0	39.0
10-11	35.71536014022654	37.0	34.5	39.0	32.0	39.0
12-13	35.82234055953012	37.5	35.5	39.0	31.5	39.0
14-15	36.36882552868092	38.0	35.5	40.0	31.0	41.0
16-17	36.55869710412307	38.0	36.0	40.0	31.0	41.0
18-19	36.54757255040185	38.0	35.5	40.0	31.5	41.0
20-21	36.75677343486208	38.5	36.0	40.0	31.0	41.0
22-23	36.668245908736324	38.0	35.5	40.5	31.5	41.0
24-25	36.632286397454585	38.0	36.0	40.5	31.0	41.0
26-27	36.40008594802684	38.0	36.5	40.0	30.0	41.0
28-29	36.28229057842387	38.0	35.0	40.0	30.0	41.0
30-31	36.1293745375795	37.5	36.0	40.0	30.0	41.0
32-33	35.89780335049119	37.5	34.5	40.0	29.5	41.0
34-35	35.76589482798222	37.5	35.0	40.0	29.5	41.0
36-37	35.630540445850094	37.5	35.0	40.0	29.0	41.0
38-39	35.44081176429495	36.5	35.0	40.0	28.5	41.0
40-41	35.4698552114459	37.0	35.0	40.0	28.5	41.0
42-43	35.169289034111415	36.0	35.0	40.0	27.0	41.0
44-45	34.95256716806544	37.0	34.5	40.0	27.0	41.0
46-47	34.50144005283899	36.5	34.0	40.0	23.5	41.0
48-49	34.181861049984285	37.0	33.5	40.0	22.0	41.0
50-51	33.15603483223728	36.5	32.5	38.5	20.0	40.0
52-53	33.47563500455672	37.0	33.5	38.5	21.0	39.5
54-55	34.36288061374512	37.5	34.0	40.0	20.5	41.0
56-57	34.39441147300311	37.0	34.5	40.0	20.0	41.0
58-59	34.36305737877571	38.0	34.0	40.0	19.5	41.0
60-61	33.88748641184982	37.0	34.0	40.0	18.0	41.0
62-63	33.64720134383762	37.5	33.0	39.0	18.5	41.0
64-65	33.279496209078104	36.0	33.0	39.0	18.0	40.5
66-67	32.90457281607863	36.5	33.0	38.5	18.0	40.0
68-69	32.483908560614	36.0	32.5	37.5	17.5	39.5
70-71	31.95021524053275	35.5	31.5	37.0	14.0	39.0
72-73	31.409208928856838	35.0	31.0	37.0	12.5	38.5
74-75	30.96328209264393	35.0	30.5	36.0	11.0	37.0
76-77	30.66645497201926	35.0	30.0	36.5	10.0	37.5
78-79	30.25024741811916	34.0	29.0	36.0	10.0	37.0
80-81	29.9020568251942	34.0	29.0	35.5	10.0	37.5
82-83	29.475945137215177	34.0	27.5	35.0	9.0	37.0
84-85	29.129792634508135	34.0	26.5	35.0	9.0	36.5
86-87	28.916142987032643	34.0	26.5	35.0	9.0	37.0
88-89	28.617301062601282	34.0	25.5	35.0	8.0	36.5
90-91	28.271924950013602	34.0	25.0	35.0	8.0	36.0
92-93	27.81476030344311	33.5	24.5	35.0	7.0	35.0
94-95	27.608229946959906	33.0	24.0	35.0	7.0	36.0
96-97	27.332779751830365	33.0	22.0	35.0	7.0	35.0
98-99	27.23319806045364	33.0	20.0	35.0	7.0	36.0
100	25.773725942226413	31.0	18.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1558631226761129
1101	2	0.03341020030843467
1101	3	0.2497434379388359
1101	4	0.34674589332879435
1101	5	0.2237418925916188
1101	6	0.20307667908399196
1101	7	0.03228826347977787
1101	8	0.16781035998286598
1101	9	0.12225064974444422
1101	10-11	0.09973836336754971
1101	12-13	0.0863266336490156
1101	14-15	0.052977030629229205
1101	16-17	0.31069061511090723
1101	18-19	0.12193187235437364
1101	20-21	0.2637150286324754
1101	22-23	0.22400400618524685
1101	24-25	0.15028943736952982
1101	26-27	0.09865999975066586
1101	28-29	0.14062076919590538
1101	30-31	0.022714956764318117
1101	32-33	-0.13459810884131684
1101	34-35	-0.05365755309742326
1101	36-37	-0.13166412197290356
1101	38-39	-0.058121134019202714
1101	40-41	-0.14661090505081376
1101	42-43	-0.15456471161796514
1101	44-45	-0.18418477777264286
1101	46-47	-0.22826620854536372
1101	48-49	-0.46761580964791705
1101	50-51	-0.5590593254537239
1101	52-53	-0.5047145423421568
1101	54-55	-0.25532511186811746
1101	56-57	-0.08310668009368527
1101	58-59	-0.16785768808735213
1101	60-61	-0.22403665705947873
1101	62-63	-0.698632434093831
1101	64-65	-0.42628536215121215
1101	66-67	-0.4977265461397664
1101	68-69	-0.5649284260623944
1101	70-71	-0.7667670147848327
1101	72-73	-0.6595526468742214
1101	74-75	-0.5560058564662036
1101	76-77	-0.4418289815924439
1101	78-79	-0.8907466051486104
1101	80-81	-0.8667149770541194
1101	82-83	-1.1285236530906246
1101	84-85	-0.9616994272534996
1101	86-87	-1.0693750896729988
1101	88-89	-1.0040808518023567
1101	90-91	-1.1005042815238149
1101	92-93	-1.3264780886642917
1101	94-95	-1.437995465376222
1101	96-97	-1.067988800279167
1101	98-99	-1.168507080033848
1101	100	-1.2162044183051108
1102	1	-0.026686954365487736
1102	2	-0.024614966615036593
1102	3	-0.03206190361226646
1102	4	-0.05424280723617869
1102	5	0.048767573125779506
1102	6	0.20007847672138013
1102	7	0.1268825110401295
1102	8	0.31804790492380164
1102	9	0.17527453264121107
1102	10-11	0.2048198990634944
1102	12-13	0.15120850319190282
1102	14-15	0.08168465774787137
1102	16-17	0.3114417707349517
1102	18-19	0.36503343886695205
1102	20-21	0.1144212433217433
1102	22-23	0.2957072676130892
1102	24-25	0.09051799412350903
1102	26-27	0.0015201692421911162
1102	28-29	0.09856247438337817
1102	30-31	0.15230535224455366
1102	32-33	0.2649524818109654
1102	34-35	-0.009162817606920726
1102	36-37	-0.4803319442636109
1102	38-39	-0.07948091830270698
1102	40-41	-0.6942001449070077
1102	42-43	-0.5637872334464191
1102	44-45	-0.07164625697140536
1102	46-47	-0.0987239640666786
1102	48-49	-0.1621426458061137
1102	50-51	-0.35863238657339735
1102	52-53	0.04111237085764685
1102	54-55	0.020214179349132166
1102	56-57	0.1041814554995355
1102	58-59	0.14208709876421466
1102	60-61	-0.16472746342825673
1102	62-63	-0.16501661488479868
1102	64-65	0.06869409342146326
1102	66-67	-0.01766748091120718
1102	68-69	0.03085675626939377
1102	70-71	0.03256529132713126
1102	72-73	-0.2012899350098145
1102	74-75	-0.17085241527462713
1102	76-77	-0.27264883264534845
1102	78-79	-0.2759578275420367
1102	80-81	-0.4817380895348613
1102	82-83	-0.55539833208395
1102	84-85	-0.6892674806690096
1102	86-87	-0.36753572141413926
1102	88-89	-0.43474982971709863
1102	90-91	-0.682874594826334
1102	92-93	-0.91912703576239
1102	94-95	-0.8961650853043146
1102	96-97	-1.2787200534892307
1102	98-99	-1.2007361503985123
1102	100	-0.9052516704879565
1103	1	-0.07600165263968606
1103	2	0.01033606487666816
1103	3	-0.31955144713053585
1103	4	0.0275545493652416
1103	5	0.1403032924673724
1103	6	0.13412606819146333
1103	7	-8.39713327010827E-4
1103	8	-0.0923081945832962
1103	9	-0.02509610941893925
1103	10-11	-0.2099414357627012
1103	12-13	-0.0721792681940201
1103	14-15	0.035707668442434226
1103	16-17	-0.19018171870560963
1103	18-19	-0.23701796197985203
1103	20-21	-0.37062437911239954
1103	22-23	-0.2853159876022815
1103	24-25	-0.37583916068390266
1103	26-27	-0.17128590002153743
1103	28-29	-0.24618032833981118
1103	30-31	-0.35903333480743527
1103	32-33	-0.41699094379679025
1103	34-35	-0.5411873211653386
1103	36-37	-0.8372126309809858
1103	38-39	-0.5868667335222
1103	40-41	-0.3315236716691672
1103	42-43	-0.41303307981013404
1103	44-45	-0.46486659595446156
1103	46-47	-0.5448470223784483
1103	48-49	-0.7546744656843671
1103	50-51	-0.8520560639088899
1103	52-53	-0.5861755073452599
1103	54-55	-0.5198363978486569
1103	56-57	-0.35451898077705124
1103	58-59	-0.5329151534010848
1103	60-61	-0.5686784048333422
1103	62-63	-0.5939627302213992
1103	64-65	-0.5478374433274453
1103	66-67	-0.4956091305257644
1103	68-69	-0.5798577592576706
1103	70-71	-0.562967056201277
1103	72-73	-0.44648317079221655
1103	74-75	-0.4730267199990301
1103	76-77	-0.48111118287635435
1103	78-79	-0.4377115564842917
1103	80-81	-0.5163525831837568
1103	82-83	-0.6089181447037966
1103	84-85	-0.6953451095893897
1103	86-87	-1.366927636784201
1103	88-89	-1.0675696693629284
1103	90-91	-1.2322137451808892
1103	92-93	-0.6043850052101618
1103	94-95	-0.9774122700976946
1103	96-97	-0.7859855387343302
1103	98-99	-0.6602962703424033
1103	100	-0.9484108792701136
1104	1	-0.04486221581518279
1104	2	-0.016215677732898115
1104	3	-0.15455005692016854
1104	4	0.0960460053108747
1104	5	-0.03920985331085092
1104	6	0.07149900423923583
1104	7	0.058112858817253255
1104	8	0.07542132404680046
1104	9	0.04432002773487653
1104	10-11	-0.034536425393518755
1104	12-13	-0.03171011683455305
1104	14-15	-0.37081216309977805
1104	16-17	-0.08155080807956239
1104	18-19	-0.08996431516471404
1104	20-21	-0.09555148872474462
1104	22-23	-0.0014280156003607658
1104	24-25	-0.06038703621288022
1104	26-27	0.020363527186283648
1104	28-29	0.06136417752329493
1104	30-31	-0.11682871624799418
1104	32-33	-0.15882441445060635
1104	34-35	-0.01045219322772084
1104	36-37	0.0025075923709820813
1104	38-39	0.004086185536948506
1104	40-41	0.016655790459715547
1104	42-43	-0.1667943003383101
1104	44-45	-0.04101441230384495
1104	46-47	0.29634376295010867
1104	48-49	0.36272433592878883
1104	50-51	0.21124569846971752
1104	52-53	0.3465147155220478
1104	54-55	0.29878004948788117
1104	56-57	0.25054393580777656
1104	58-59	0.28105939427037896
1104	60-61	0.13640058207317196
1104	62-63	0.11060608774267422
1104	64-65	-0.014249833290378433
1104	66-67	0.28381422551858293
1104	68-69	0.0859133483446115
1104	70-71	0.19030312671979743
1104	72-73	0.07310412804179123
1104	74-75	-0.029759419532478404
1104	76-77	0.024221995705730137
1104	78-79	-0.17514864627657545
1104	80-81	-0.29254720753360886
1104	82-83	-0.331926381381372
1104	84-85	-0.22747108560634643
1104	86-87	-0.22208238377866962
1104	88-89	-0.22095578817617323
1104	90-91	-0.29348758601327773
1104	92-93	-0.12377397586779537
1104	94-95	-0.40445492277212836
1104	96-97	-0.5717879196927882
1104	98-99	-0.4270278985210716
1104	100	-0.27812796508308324
1105	1	0.23861713337129942
1105	2	0.12373996857937897
1105	3	0.03947902914501711
1105	4	0.27893543581245694
1105	5	0.2432655606447014
1105	6	0.2251181724490081
1105	7	0.11904306641105222
1105	8	0.1158470999313721
1105	9	0.02861082007520821
1105	10-11	0.11344033405481468
1105	12-13	0.15734564731508272
1105	14-15	0.18923792387541738
1105	16-17	-0.022585776253713163
1105	18-19	0.19873049584417402
1105	20-21	0.07041437503770709
1105	22-23	0.24195186714781158
1105	24-25	0.1261906059177491
1105	26-27	-0.011103072144756254
1105	28-29	0.21536576820560782
1105	30-31	0.046489466568246485
1105	32-33	0.22038802702716964
1105	34-35	0.15437250235910938
1105	36-37	0.14498100577516482
1105	38-39	0.09442144867044533
1105	40-41	0.08183655979370741
1105	42-43	0.3800423887979605
1105	44-45	0.05092861101721269
1105	46-47	0.07315115132787753
1105	48-49	0.08435859122354117
1105	50-51	0.10899251649517083
1105	52-53	0.09924168661407862
1105	54-55	0.35695949894590484
1105	56-57	0.3659129277494557
1105	58-59	0.39846980250484165
1105	60-61	0.4119259097854382
1105	62-63	0.5381867380194549
1105	64-65	0.3866033286786532
1105	66-67	0.5231637291622775
1105	68-69	0.5647569076670536
1105	70-71	0.40666336746907916
1105	72-73	0.25665105682947953
1105	74-75	0.27051053291783234
1105	76-77	0.24368919032278313
1105	78-79	0.4131415410189483
1105	80-81	0.2572006498026802
1105	82-83	0.11542069232430663
1105	84-85	-0.06717080772487805
1105	86-87	-0.08269579674370675
1105	88-89	-0.21800192528225537
1105	90-91	-0.19399309118909613
1105	92-93	-0.21423061391681486
1105	94-95	-0.3023188462437183
1105	96-97	-0.11850994307972229
1105	98-99	-0.3961462164965148
1105	100	-0.24597764072539263
1106	1	-0.30685773410011663
1106	2	-0.43639287934806603
1106	3	-0.2825707462483109
1106	4	-0.25192403066686353
1106	5	-0.4051274194210137
1106	6	-0.2757050305202
1106	7	-0.2106319813134263
1106	8	-1.4186458141361697
1106	9	-0.012826094900702856
1106	10-11	-0.3208081029240333
1106	12-13	-0.40992990568570775
1106	14-15	-0.4705503852231061
1106	16-17	-0.4038032650588619
1106	18-19	-0.2629887919433287
1106	20-21	-0.16410798982571606
1106	22-23	-0.30341122923079666
1106	24-25	-0.44901714638330503
1106	26-27	-0.3418869600756054
1106	28-29	-0.5756843833063172
1106	30-31	-0.6196981811292659
1106	32-33	-0.4586603929198887
1106	34-35	-0.41869740602408
1106	36-37	-0.43468517122767025
1106	38-39	-0.46106136696757005
1106	40-41	-0.6088857298460013
1106	42-43	-0.5952029753629091
1106	44-45	-0.7211098098523934
1106	46-47	-0.4422856784890996
1106	48-49	-0.748811520831417
1106	50-51	-0.7100926637224063
1106	52-53	-0.7143063891489874
1106	54-55	-0.5678238780313833
1106	56-57	-0.7534106974661015
1106	58-59	-0.8359691775750662
1106	60-61	-0.7352127502388299
1106	62-63	-0.48956620173769494
1106	64-65	-0.49841792557042197
1106	66-67	-0.571680252585324
1106	68-69	-0.6227861153329499
1106	70-71	-0.6103559990904301
1106	72-73	-0.47528283145620165
1106	74-75	-0.6135970370011954
1106	76-77	-0.7876049415174293
1106	78-79	-0.8936541414234114
1106	80-81	-0.6152738557432933
1106	82-83	-0.610206112867548
1106	84-85	-0.6423306581290298
1106	86-87	-0.830886169526913
1106	88-89	-0.7915561750675835
1106	90-91	-0.9727750346577615
1106	92-93	-0.6185013341484513
1106	94-95	-0.9478075520409135
1106	96-97	-0.9265954284501241
1106	98-99	-0.8083402653067715
1106	100	-0.7547667517371508
1107	1	-0.1196860584555921
1107	2	0.007615849028645982
1107	3	-0.0250555225397342
1107	4	0.09040824500085165
1107	5	0.03883211520611951
1107	6	0.1552427573423003
1107	7	0.20653804282665078
1107	8	0.10203488680448203
1107	9	0.26001298180289467
1107	10-11	0.15893285684602887
1107	12-13	0.07548766140227059
1107	14-15	0.0430727695720563
1107	16-17	0.0952093461102308
1107	18-19	0.14184765640850827
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2314	1	-0.3103643269170959
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2314	92-93	0.8575376524039768
2314	94-95	0.7779467878957966
2314	96-97	0.9497000041071111
2314	98-99	1.0537957144479329
2314	100	1.1222373928020701
2315	1	0.05256865057760507
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2315	4	0.14945963012034014
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2315	6	0.17172243054674396
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2315	10-11	-1.186420596548615
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2315	18-19	0.301053237300664
2315	20-21	0.2805017721483267
2315	22-23	0.24727762857630609
2315	24-25	0.19447116822272648
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2315	100	0.5288680644474866
2316	1	0.12614011131247693
2316	2	0.07799471735388863
2316	3	0.019807358393382657
2316	4	0.17975370582879435
2316	5	0.23512328463707632
2316	6	0.2858714234021704
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2316	9	0.144391984971719
2316	10-11	-0.01332981845062875
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2316	30-31	0.0447852692643238
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2316	34-35	0.3005239099707637
2316	36-37	0.3717094575725497
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2316	52-53	0.3411217502714834
2316	54-55	0.21132083983642502
2316	56-57	0.4360339449063204
2316	58-59	0.1665883346399184
2316	60-61	0.21578045941779322
2316	62-63	0.30644569090616613
2316	64-65	-0.1098436007875776
2316	66-67	0.06247409306477891
2316	68-69	0.416783221664879
2316	70-71	0.44864491703334863
2316	72-73	0.37472469687577714
2316	74-75	0.32718839069288563
2316	76-77	0.4129206632939173
2316	78-79	0.3220818749650256
2316	80-81	0.7577878638549684
2316	82-83	0.8637792588411912
2316	84-85	0.6194973057010458
2316	86-87	0.6133219983951825
2316	88-89	0.9480764635385519
2316	90-91	0.9723916701807305
2316	92-93	0.9710449937220709
2316	94-95	1.0905911823510515
2316	96-97	1.088145787789017
2316	98-99	1.1551701216706967
2316	100	0.9021194453312518
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1606.0
1	127.0
2	89.0
3	78.0
4	62.0
5	76.0
6	71.0
7	188.0
8	682.0
9	1435.0
10	2293.0
11	2906.0
12	2873.0
13	2584.0
14	2503.0
15	2905.0
16	4173.0
17	5791.0
18	7305.0
19	9496.0
20	12142.0
21	14173.0
22	13618.0
23	12043.0
24	11679.0
25	11817.0
26	12858.0
27	14916.0
28	17787.0
29	21573.0
30	26427.0
31	33392.0
32	42754.0
33	56760.0
34	79922.0
35	128180.0
36	138925.0
37	144202.0
38	97374.0
39	6970.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.664996141680874	16.904949672254872	12.999355533508016	17.43069865255624
2	14.166750520334073	25.75497428781756	32.055544669289965	28.022730522558405
3	29.698342620025496	27.266251888211507	29.354298009933878	13.681107481829116
4	13.910960089254342	18.644476219896035	35.47520400158382	31.969359689265808
5	10.02257930623038	41.49051021588874	33.55229704897915	14.934613428901725
6	27.08350355204448	31.97158364414828	15.9518895603317	24.99302324347554
7	20.04083100864688	36.90579900065894	22.646884990890484	20.4064849998037
8	26.66665959259727	24.331049470028407	21.185069327648613	27.817221609725713
9	24.33185895177414	20.82496742069539	23.883283314726693	30.959890312803772
10-11	25.578034758741275	25.17454434919798	22.89619750340972	26.351223388651025
12-13	33.459125102593774	24.025750439952866	22.994958248910663	19.5201662085427
14-15	28.61834672607208	24.546795024796456	28.7971464184369	18.037711830694565
16-17	19.625223845128875	28.864511105264494	31.33662063873306	20.17364441087357
18-19	20.749372949619527	30.853372066868396	30.09008942099165	18.307165562520424
20-21	23.11158950831778	24.834243855961653	27.266182775790742	24.787983859929824
22-23	19.761582827767914	26.86237887780656	27.302389211020895	26.07364908340464
24-25	25.743107868469313	27.761878718854717	23.638455576909507	22.856557835766463
26-27	24.06667508336242	21.79538729708425	31.210115736639466	22.92782188291386
28-29	20.346521075045082	28.94996941493665	29.463575656517804	21.239933853500464
30-31	23.711274034725328	25.508968313708763	27.55025231587943	23.22950533568648
32-33	20.004191181541824	26.819052368548103	31.44861027847484	21.72814617143524
34-35	20.626396691382986	26.050479005802384	30.22889693140306	23.09422737141157
36-37	22.17020691952646	24.966142900565423	31.660326693823077	21.203323486085036
38-39	23.59778541999201	25.254741947958326	29.559508217678975	21.587964414370685
40-41	20.053564029335007	28.52475559024154	28.3887763834412	23.03290399698225
42-43	21.726633970892095	27.540575739169416	25.59065915848609	25.1421311314524
44-45	24.706026503098464	22.882645578476296	27.237627758927147	25.17370015949809
46-47	23.7816250233395	27.900251217897576	30.39540296708706	17.92272079167586
48-49	22.768233934186117	26.04094442407945	31.028363809207537	20.162457832526897
50-51	16.626945838754832	27.90815603946657	33.67500494631721	21.789893175461387
52-53	19.12969942363357	30.377651582673355	25.159476941098575	25.3331720525945
54-55	17.96451823935677	26.88377142135559	26.382058455715185	28.769651883572458
56-57	22.99955010303065	23.388010670197936	26.09954182662414	27.512897400147278
58-59	26.279567245455354	22.34223728497031	27.68131889120442	23.696876578369913
60-61	22.659481583405917	25.583253147408076	30.71829968353524	21.038965585650768
62-63	17.794064276785353	26.23970406276993	31.35116865917768	24.61506300126703
64-65	16.40340154245137	30.760052037572315	28.744126672863636	24.09241974711268
66-67	21.690191363441976	25.709322401715724	24.7973933058345	27.803092929007807
68-69	21.405095759052458	24.505233399093505	26.617323543238818	27.47234729861522
70-71	24.0542802658179	25.58054122104061	25.035785424385676	25.329393088755815
72-73	24.022686196399615	21.930290637034126	30.81734533284854	23.22967783371772
74-75	22.225937034985048	27.760583501561282	26.361850358850912	23.65162910460276
76-77	21.236801788965533	24.563040180085235	28.466850381625108	25.73330764932412
78-79	23.028436529460365	24.78596060773126	29.765789036619772	22.419813826188605
80-81	24.09760642079373	23.980410382433647	27.783366120636053	24.138617076136573
82-83	21.3695965566952	23.715273669404326	29.418990067920586	25.496139705979886
84-85	21.82831088417252	26.339175315722834	27.057757192452158	24.77475660765249
86-87	22.81899708145397	25.23427964977448	26.40323693287344	25.543486335898113
88-89	22.61046766169154	24.18303150912106	25.96770812603648	27.23879270315091
90-91	25.455856647531355	22.30771026822339	26.179559919638052	26.056873164607207
92-93	25.087711386696732	24.609589495324624	27.681623449830887	22.621075668147757
94-95	22.99300653286091	23.83552324708287	28.308922441446494	24.862547778609724
96-97	22.296270078752276	24.02944627190946	28.978247476674397	24.69603617266387
98-99	25.88990925957661	24.32302805509471	24.25994534246379	25.52711734286489
100	24.924168292263573	20.264418637725026	25.855905958069155	28.955507111942246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1067.0
1	1037.0
2	805.5
3	484.0
4	318.0
5	304.0
6	429.5
7	731.5
8	1119.5
9	1315.0
10	1152.5
11	892.0
12	876.5
13	903.5
14	931.0
15	1025.5
16	1071.5
17	1134.5
18	1265.5
19	1422.0
20	1562.0
21	1787.5
22	2076.0
23	2363.0
24	2703.0
25	3202.5
26	3894.0
27	4727.5
28	5537.5
29	6347.0
30	7111.0
31	7655.0
32	8333.0
33	8868.0
34	8976.5
35	8985.5
36	8459.0
37	7666.5
38	7239.0
39	6741.0
40	6119.5
41	5897.5
42	5857.5
43	6233.5
44	7039.5
45	9300.5
46	21062.0
47	60543.0
48	126740.5
49	147506.5
50	149949.0
51	124654.5
52	56868.5
53	26503.5
54	16878.0
55	10897.0
56	6844.5
57	4485.5
58	3281.5
59	2656.0
60	2314.5
61	2034.0
62	1782.5
63	1403.5
64	1007.0
65	788.0
66	689.0
67	569.0
68	434.5
69	372.0
70	348.5
71	320.0
72	265.5
73	227.0
74	191.5
75	166.5
76	135.5
77	102.5
78	80.0
79	48.5
80	38.5
81	35.5
82	23.0
83	17.0
84	17.0
85	14.5
86	11.0
87	7.5
88	5.0
89	2.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14194126108618407
2	0.06901245505458017
3	0.28875149906272196
4	0.28896319371012863
5	0.2904450562419755
6	0.24789443211323126
7	0.2472593481710112
8	0.24852951605545132
9	0.2583733171598623
10-11	0.23619830284401172
12-13	0.2462537985958294
14-15	0.22037412795036185
16-17	0.22661912004885912
18-19	0.23805063100882026
20-21	0.23900325692215035
22-23	0.22921237947959106
24-25	0.2305883946877345
26-27	0.23138224961550957
28-29	0.2438193101506525
30-31	0.23164686792476796
32-33	0.24366053916509747
34-35	0.225190181178864
36-37	0.23207025721958133
38-39	0.23132932595365793
40-41	0.2469947298617528
42-43	0.23598660819660505
44-45	0.22360247132331385
46-47	0.2274658986384859
48-49	0.2366216921388251
50-51	0.2261428070921941
52-53	0.24355469184139414
54-55	0.2524987906943267
56-57	0.24545994366805432
58-59	0.24651841690508774
60-61	0.22820682990440927
62-63	0.25350434026950847
64-65	0.24990553126359474
66-67	0.255515439419872
68-69	0.25869085913097234
70-71	0.28404129315792315
72-73	0.29367339961492744
74-75	0.27340363712573706
76-77	0.2684817365735316
78-79	0.2609665765905942
80-81	0.24545994366805432
82-83	0.2547215844920969
84-85	0.2703340647383401
86-87	0.26535924052428295
88-89	0.27197469825574194
90-91	0.26673525573242646
92-93	0.24551286732990601
94-95	0.26678817939427807
96-97	0.26445953827280455
98-99	0.24805320309878626
100	0.26874635488279
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	944757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.81476801643352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89849640043576	13.521141061698774
2	9.472180745524843	3.3748940518250676
3	4.063205656064266	2.1715519849753635
4	2.349264552369901	1.6740641203880124
5	1.5301154360287113	1.3629325765607754
6	1.049770233037485	1.122084790327209
7	0.809716947343151	1.0097443703125064
8	0.6117560762256363	0.8718634064482663
9	0.502321125774773	0.8053860892888173
>10	3.230758295349191	10.66228096523026
>50	0.24061315935682304	2.9334255909916065
>100	0.1752614370623773	6.085937304560519
>500	0.020793729820960016	2.614110213302846
>1k	0.027328902050404592	12.686674077824431
>5k	0.010099811627323436	13.647266178554688
>10k+	0.008317491928384007	25.456643217710862
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	41731	4.417114665464241	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	26877	2.844858519174772	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	26800	2.8367082752496144	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	24221	2.5637280274186907	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	17307	1.8318996313337714	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	14289	1.5124524083970798	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	12061	1.2766245711860298	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	11831	1.252279686734261	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	11546	1.2221131994788077	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	11481	1.2152331234380904	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	10937	1.1576521793434713	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	10797	1.1428335540250032	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	10451	1.1062103800236462	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	10194	1.0790076178318868	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	9939	1.0520165502875343	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	9874	1.045136474246817	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	9498	1.0053378805343596	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	9310	0.985438583678131	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	9014	0.9541077758619412	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	8617	0.9120863883517137	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8457	0.8951508165591787	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	7497	0.7935373858039687	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	7461	0.7897268821506483	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	7307	0.7734263943003333	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7197	0.7617831886929655	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	6600	0.6985923364420692	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5869	0.6212179428149248	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	5761	0.6097864318549637	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5727	0.60618762284905	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	5713	0.6047057603172032	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5103	0.5401388928581635	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	4903	0.5189694281174948	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	4574	0.48414565861909464	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	4364	0.46191772064139247	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	4164	0.4407482559007237	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	4051	0.4287875083222458	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	4017	0.42518869931633213	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	4006	0.4240243787555954	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	3940	0.4170384553911746	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	3874	0.41005253202675396	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	3840	0.40645372302084026	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	3697	0.3913175557312621	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	3673	0.38877721996238185	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	3508	0.3713124115513301	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3506	0.37110071690392343	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	3445	0.3646440301580195	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	3287	0.34792015301289114	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	3237	0.342627786827724	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	3124	0.3306670392492461	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3016	0.319235528289285	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	2878	0.3046285976182235	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	2822	0.29870114749083626	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	2618	0.2771082934553541	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	2557	0.27065160670945015	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	2473	0.2617604315183693	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	2260	0.23921495156955705	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	2162	0.22884191384662933	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	2137	0.22619573075404573	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2075	0.21963319668443843	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	2072	0.2193156547133284	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1853	0.1961350908222961	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1799	0.19041933534231553	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	1696	0.17951706100087114	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	1568	0.16596860356684312	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	1557	0.16480428300610633	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.16406335174018294	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	1508	0.1596177641446425	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	1500	0.15877098555501573	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	1457	0.15421955063577195	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	1287	0.1362255056062035	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	1254	0.13273254392399317	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	1225	0.1296629715365962	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1199	0.12691094112030923	No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC	1107	0.11717298733960162	No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1012	0.10711749158778396	No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC	1009	0.10679994961667393	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	1007	0.10658825496926722	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	994	0.10521223976112375	No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA	971	0.10277775131594685	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0584732370334382E-4	0.0
5	0.0	0.0	0.0	1.0584732370334382E-4	0.0
6	0.0	0.0	0.0	1.0584732370334382E-4	0.0
7	0.0	0.0	0.0	1.0584732370334382E-4	0.0
8	0.0	0.0	0.0	1.0584732370334382E-4	0.0
9	0.0	0.0	0.0	2.1169464740668765E-4	0.0
10-11	0.0	0.0	0.0	2.1169464740668765E-4	0.0
12-13	0.0	0.0	0.0	2.1169464740668765E-4	0.0
14-15	0.0	0.0	0.0	4.233892948133753E-4	0.0
16-17	0.0	0.0	0.0	6.35083942220063E-4	1.0584732370334382E-4
18-19	0.0	0.0	0.0	8.997022514784226E-4	1.0584732370334382E-4
20-21	1.0584732370334382E-4	0.0	0.0	0.002275717459621892	1.0584732370334382E-4
22-23	1.0584732370334382E-4	0.0	0.0	0.007038847026272364	1.0584732370334382E-4
24-25	1.0584732370334382E-4	0.0	0.0	0.01720019010179337	1.0584732370334382E-4
26-27	1.0584732370334382E-4	0.0	0.0	0.04022198300727065	1.0584732370334382E-4
28-29	1.0584732370334382E-4	0.0	0.0	0.10780549919185567	1.0584732370334382E-4
30-31	1.0584732370334382E-4	0.0	0.0	0.2639832253161395	1.0584732370334382E-4
32-33	1.0584732370334382E-4	0.0	0.0	0.564748395619191	1.0584732370334382E-4
34-35	1.0584732370334382E-4	0.0	0.0	1.0900686631588865	1.0584732370334382E-4
36-37	1.0584732370334382E-4	0.0	0.0	1.8283537459897095	1.0584732370334382E-4
38-39	1.0584732370334382E-4	0.0	0.0	2.7528242712147146	1.0584732370334382E-4
40-41	1.0584732370334382E-4	0.0	0.0	3.858134948986882	1.0584732370334382E-4
42-43	1.0584732370334382E-4	0.0	0.0	4.8115017935829005	1.0584732370334382E-4
44-45	1.0584732370334382E-4	0.0	0.0	5.731844273183475	1.0584732370334382E-4
46-47	1.0584732370334382E-4	0.0	0.0	6.827999157455303	1.0584732370334382E-4
48-49	1.0584732370334382E-4	0.0	0.0	7.817248244786754	1.0584732370334382E-4
50-51	1.0584732370334382E-4	0.0	0.0	8.772308646562026	1.0584732370334382E-4
52-53	1.0584732370334382E-4	0.0	0.0	9.72239422412324	1.0584732370334382E-4
54-55	1.0584732370334382E-4	0.0	0.0	10.780126529890754	1.0584732370334382E-4
56-57	1.0584732370334382E-4	0.0	0.0	12.027748934382068	1.0584732370334382E-4
58-59	1.0584732370334382E-4	0.0	0.0	13.01096472426243	1.0584732370334382E-4
60-61	1.0584732370334382E-4	0.0	0.0	13.895583732113126	1.0584732370334382E-4
62-63	1.0584732370334382E-4	0.0	0.0	14.722039635588834	1.0584732370334382E-4
64-65	1.0584732370334382E-4	0.0	0.0	15.637248519989797	1.0584732370334382E-4
66-67	1.0584732370334382E-4	0.0	0.0	16.646555675163032	1.0584732370334382E-4
68-69	1.0584732370334382E-4	0.0	0.0	17.864646676341113	1.0584732370334382E-4
70-71	1.0584732370334382E-4	0.0	0.0	19.291468599862185	1.0584732370334382E-4
72-73	1.0584732370334382E-4	0.0	0.0	20.385929926954763	1.0584732370334382E-4
74-75	1.0584732370334382E-4	0.0	0.0	21.48134387996067	1.0584732370334382E-4
76-77	1.0584732370334382E-4	0.0	0.0	22.533783819543018	1.0584732370334382E-4
78-79	1.0584732370334382E-4	0.0	0.0	23.90641191332798	1.0584732370334382E-4
80-81	1.0584732370334382E-4	0.0	0.0	25.19928404870247	1.0584732370334382E-4
82-83	1.0584732370334382E-4	0.0	0.0	26.503005534756554	1.0584732370334382E-4
84-85	1.0584732370334382E-4	0.0	0.0	27.839327996511273	1.0584732370334382E-4
86-87	1.0584732370334382E-4	0.0	0.0	29.003701480909907	1.0584732370334382E-4
88	1.0584732370334382E-4	0.0	0.0	30.05005519937931	1.0584732370334382E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCA	560	0.0	57.10718	1
CCGCATC	40	5.670566E-4	47.009453	94
TACCCAT	685	0.0	46.67872	2
ACCCATG	755	0.0	42.35089	3
ACTGAGT	45	0.0010107426	41.80395	1
CTATAAC	50	0.0016966437	37.623554	1
ACGCTAA	50	0.0017024095	37.597576	6
CTAACGC	55	0.0027105482	34.197777	3
TTATGTA	465	0.0	33.370415	3
CTTATGT	460	0.0	32.71092	2
CCCTAAC	60	0.004147869	31.35296	1
CCTAACG	75	3.266141E-4	31.347965	2
CATTGTA	60	0.0041511073	31.347963	2
AACGCTA	60	0.004152187	31.346296	5
TGTGGTC	30	0.0041559334	31.342136	86-87
GCCGGAT	90	2.5898666E-5	31.339636	94
TCGTCGT	45	2.5912323E-5	31.339636	90-91
GCTTATG	520	0.0	28.941195	1
AGTTCCC	65	0.0061411853	28.935045	5
TAACGCT	65	0.0061427797	28.933508	4
>>END_MODULE