##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523097_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	944757
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00149244726422	33.0	31.0	34.0	30.0	34.0
2	32.04245959543036	33.0	31.0	34.0	30.0	34.0
3	32.22014338078469	33.0	31.0	34.0	30.0	35.0
4	35.294641902626815	37.0	35.0	37.0	32.0	37.0
5	35.241189004156624	36.0	35.0	37.0	32.0	37.0
6	35.48670292995977	36.0	35.0	37.0	33.0	37.0
7	35.31818023047196	36.0	35.0	37.0	32.0	37.0
8	35.38101014334903	36.0	35.0	37.0	33.0	37.0
9	36.50711241091625	37.0	35.0	39.0	33.0	39.0
10-11	36.31995740703694	37.0	35.0	39.0	32.0	39.0
12-13	36.392227842715116	37.0	35.0	39.0	32.5	39.0
14-15	37.24570921411538	38.0	36.5	40.0	32.0	41.0
16-17	37.08211688296568	38.0	36.0	40.0	32.0	41.0
18-19	37.11064009052063	38.0	36.0	40.0	32.0	41.0
20-21	37.22968763396302	38.0	36.0	40.0	32.5	41.0
22-23	37.158230105730894	38.0	35.5	40.0	32.0	41.0
24-25	37.01604539580019	38.0	36.5	40.0	31.5	41.0
26-27	36.57523098532215	37.0	35.5	40.0	31.0	41.0
28-29	36.644034921149036	38.0	35.5	40.0	31.0	41.0
30-31	36.42896374411622	37.0	35.5	40.0	31.0	41.0
32-33	36.17176215682974	37.0	35.0	40.0	30.0	41.0
34-35	35.9448688922125	37.0	35.0	40.0	30.0	41.0
36-37	35.79673397497981	37.0	35.0	40.0	30.0	41.0
38-39	35.68994037620256	36.5	34.5	40.0	30.0	41.0
40-41	35.9443460064334	37.5	35.0	40.0	30.0	41.0
42-43	35.91747137094512	37.5	35.0	40.0	30.0	41.0
44-45	35.66221843288804	37.0	35.0	40.0	29.5	41.0
46-47	35.39895020624351	37.0	35.0	40.0	27.0	41.0
48-49	35.103742020434886	37.5	34.5	40.0	26.5	41.0
50-51	34.868493697321114	37.5	34.0	40.0	25.0	41.0
52-53	35.035268328257956	37.5	35.0	40.0	26.0	41.0
54-55	34.9377009114513	37.5	34.0	40.0	26.0	41.0
56-57	34.55391703898463	37.0	34.5	40.0	23.5	41.0
58-59	34.37294881117579	37.0	34.0	40.0	23.5	41.0
60-61	33.92496112757037	36.5	33.0	39.0	22.0	41.0
62-63	33.644248732742916	36.5	32.5	39.0	22.0	41.0
64-65	33.52662324809448	37.0	32.5	39.0	22.0	40.0
66-67	32.825723439995684	36.0	32.5	37.5	20.5	40.0
68-69	32.58678474994099	36.5	32.0	37.0	19.0	39.0
70-71	31.91988733610865	35.0	31.5	37.0	18.0	39.0
72-73	31.55899347662944	35.0	31.0	36.0	17.0	38.0
74-75	31.387381093762734	35.0	31.0	37.0	15.5	38.0
76-77	29.790819755767885	33.5	28.0	35.5	12.0	36.0
78-79	30.771255465691176	34.0	30.0	36.0	13.0	37.0
80-81	30.86329130136109	34.0	31.0	36.0	15.0	37.5
82-83	30.807981311596528	34.0	30.5	36.0	13.5	37.0
84-85	30.499169627745548	34.0	30.0	35.5	13.0	36.0
86-87	30.496874328531042	34.0	30.0	35.0	12.0	36.0
88-89	30.41657008098379	34.0	30.0	35.0	11.0	37.0
90-91	30.14619949891877	34.0	29.0	35.0	11.0	36.5
92-93	29.899542951256247	34.0	29.0	35.0	10.5	36.5
94-95	30.039214316485616	34.0	29.0	35.0	11.0	36.5
96-97	29.809076831396858	34.0	29.0	35.0	11.0	37.0
98-99	29.71080288370449	34.0	29.0	35.0	10.0	36.0
100	28.412206525064118	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.16205646118609351
1101	2	-0.19016261015863734
1101	3	-0.08709108843519431
1101	4	-0.04527504959926887
1101	5	-0.25045731159529794
1101	6	-0.14689272108494578
1101	7	-0.06382546276059742
1101	8	-0.23457062547642238
1101	9	-0.25952117138410813
1101	10-11	-0.2403998627798387
1101	12-13	-0.09846384136604058
1101	14-15	-0.32133022469207617
1101	16-17	-0.31401576644772433
1101	18-19	-0.49639849470352715
1101	20-21	-0.3710949228881546
1101	22-23	-0.8549919793552689
1101	24-25	-0.3513043194940906
1101	26-27	-0.44975385175929006
1101	28-29	-0.12641555192288934
1101	30-31	-0.3810392969671952
1101	32-33	-0.11561743047690953
1101	34-35	-0.19789187843620226
1101	36-37	-0.3506128619688056
1101	38-39	-0.4560618268479857
1101	40-41	-0.5266001308023505
1101	42-43	-0.5018565702738584
1101	44-45	-0.49457151722088355
1101	46-47	-0.3778340394870767
1101	48-49	-0.5636961994708329
1101	50-51	-0.28087124820017095
1101	52-53	-0.23116054024747257
1101	54-55	-0.2843319014106598
1101	56-57	-0.5486114359232559
1101	58-59	-0.40012735578548586
1101	60-61	-0.7264524748682746
1101	62-63	-0.36919661718627594
1101	64-65	-0.31482581910652385
1101	66-67	-0.4137899459804828
1101	68-69	-0.31241428364376134
1101	70-71	-0.5638442545450957
1101	72-73	-0.5613313864253477
1101	74-75	-0.43896465935906903
1101	76-77	-0.34492287796289745
1101	78-79	-0.7109205600858175
1101	80-81	-0.6394573189224957
1101	82-83	-0.6834377003031342
1101	84-85	-0.7402444930371317
1101	86-87	-0.7741377198563235
1101	88-89	-0.7612259493817035
1101	90-91	-0.8485486378078662
1101	92-93	-0.7542932970376448
1101	94-95	-0.712190009765461
1101	96-97	-0.7259911068626401
1101	98-99	-0.959356222776865
1101	100	-1.083776883421912
1102	1	-0.02673930658927759
1102	2	-0.12831746377959163
1102	3	-0.1568201587998601
1102	4	-0.1902352447713298
1102	5	-0.1644724631104495
1102	6	-0.19968547917431323
1102	7	-0.001973896248010476
1102	8	-0.188043917720897
1102	9	0.043021201497246864
1102	10-11	-0.2623278288815314
1102	12-13	-0.05947437038504688
1102	14-15	-0.20351114405519866
1102	16-17	-0.21221170892333419
1102	18-19	-0.11228190507846136
1102	20-21	-0.16242774261080228
1102	22-23	-0.12172605742409104
1102	24-25	0.1696477863097101
1102	26-27	-0.04775077009518469
1102	28-29	-0.1474446993291565
1102	30-31	-0.1643886806343673
1102	32-33	-0.034640286152310296
1102	34-35	-0.1418023817746743
1102	36-37	-0.28311799807563176
1102	38-39	-0.3828562541720757
1102	40-41	-0.19847352576896782
1102	42-43	-0.2414559539410419
1102	44-45	-0.30615985825837555
1102	46-47	-0.35362692597911405
1102	48-49	-0.6090159221210598
1102	50-51	-0.5027285928329377
1102	52-53	-0.5323354246850727
1102	54-55	-0.5089499034651013
1102	56-57	-0.5023928945775893
1102	58-59	-0.7463202165969918
1102	60-61	-0.2948076366556407
1102	62-63	-0.3424792314646581
1102	64-65	-0.4075357574732337
1102	66-67	-0.4132057138284537
1102	68-69	-0.1521998511835605
1102	70-71	-0.4245883480222403
1102	72-73	-0.4273624598716772
1102	74-75	-0.22964262546076597
1102	76-77	-0.3834404691287894
1102	78-79	-0.3427676325049269
1102	80-81	-0.19097247043764654
1102	82-83	-0.38521928736014743
1102	84-85	-0.2393649347423228
1102	86-87	-0.19444781230624741
1102	88-89	-0.22410473726049318
1102	90-91	-0.4383502556815202
1102	92-93	-0.21441784762829386
1102	94-95	-0.6513624545523164
1102	96-97	-0.7541209219627909
1102	98-99	-0.6121361405991586
1102	100	-0.6132902372996725
1103	1	-0.24896109982763193
1103	2	-0.2018860518649923
1103	3	-0.39428460676196764
1103	4	-0.25197674767340317
1103	5	-0.1878589475447754
1103	6	-0.25886466144526565
1103	7	-0.08147404424744309
1103	8	-0.1738308242753419
1103	9	-0.2281999868738609
1103	10-11	-0.2362284504791532
1103	12-13	-0.12284000620347513
1103	14-15	-0.16769463761400516
1103	16-17	-0.2478866506887698
1103	18-19	-0.34580313334507196
1103	20-21	-0.2626304374874522
1103	22-23	-0.2705684444619223
1103	24-25	-0.25747407513708254
1103	26-27	-0.2757323152092752
1103	28-29	-0.25051086151079716
1103	30-31	-0.48342462519249096
1103	32-33	-0.3979248440200891
1103	34-35	-0.602738896452351
1103	36-37	-0.6924046408050017
1103	38-39	-0.5449169210703886
1103	40-41	-0.4032024294149039
1103	42-43	-0.4627698028271823
1103	44-45	-0.26529785807819906
1103	46-47	-0.3043366797852727
1103	48-49	-0.16558323612438386
1103	50-51	-0.3780950523004023
1103	52-53	-0.30961622465418515
1103	54-55	-1.2560690623135358
1103	56-57	-0.45118702093049734
1103	58-59	-0.46111565564052626
1103	60-61	-0.5574345622569723
1103	62-63	-0.44545852441344636
1103	64-65	-0.41628445443474504
1103	66-67	-0.44591616258848177
1103	68-69	-0.37963058101382074
1103	70-71	-0.3671433330292544
1103	72-73	-0.494731158634913
1103	74-75	-0.5381673928442687
1103	76-77	-0.39465496535158806
1103	78-79	-0.3691507837346073
1103	80-81	-1.3018304810678671
1103	82-83	-0.29422280073800877
1103	84-85	-0.2516469573154545
1103	86-87	-0.31355711310544265
1103	88-89	-0.20170534470164725
1103	90-91	-0.4623416073667812
1103	92-93	-0.466953785751663
1103	94-95	-0.649357383963018
1103	96-97	-0.5737610902960633
1103	98-99	-0.4850007661605886
1103	100	-0.3632213853891919
1104	1	-0.1374598511891456
1104	2	-0.31568816243390785
1104	3	-0.12723284757191067
1104	4	-0.15653306649838328
1104	5	-0.046368693046993315
1104	6	-0.4755334113017824
1104	7	-0.18843861556523933
1104	8	-0.17537918701566468
1104	9	-0.2844371848219609
1104	10-11	-0.24158267353011809
1104	12-13	-0.25287059083157715
1104	14-15	-0.5579464570040003
1104	16-17	-0.17980396760826522
1104	18-19	-0.38267814450500737
1104	20-21	-0.4889637002474103
1104	22-23	-0.4905520170219688
1104	24-25	-0.2964210099145248
1104	26-27	-0.39923975219704033
1104	28-29	-0.644655269931647
1104	30-31	-0.5872956595855356
1104	32-33	-0.2660895367580878
1104	34-35	-0.5187631753923228
1104	36-37	-0.8017823112205633
1104	38-39	-0.24010883387231985
1104	40-41	-0.43083381704891366
1104	42-43	-0.3795924961620756
1104	44-45	-0.5202879480464944
1104	46-47	-0.32542578844361003
1104	48-49	-0.2039881164004882
1104	50-51	-0.0142349100142809
1104	52-53	0.05774941597771033
1104	54-55	-0.04026249796975634
1104	56-57	0.08044664205699803
1104	58-59	-0.14950000161670118
1104	60-61	-0.2657416431468107
1104	62-63	-0.14973782150267567
1104	64-65	-0.303320296353796
1104	66-67	-0.12729014958427598
1104	68-69	-0.21235447503132576
1104	70-71	-0.08856111802875688
1104	72-73	-0.2072860336817861
1104	74-75	-0.06141706697385274
1104	76-77	0.016298872011656584
1104	78-79	0.037701933042555424
1104	80-81	-0.018333426018088517
1104	82-83	0.13795151378624482
1104	84-85	-0.3804193377892453
1104	86-87	-0.037893064455733594
1104	88-89	0.02524203392278679
1104	90-91	-0.016895247804814773
1104	92-93	-0.09286730701422741
1104	94-95	-0.09834431598556037
1104	96-97	0.06513240428466816
1104	98-99	0.023682309239831056
1104	100	-1.058256421241314
1105	1	0.02611693948730931
1105	2	-0.19934512154702944
1105	3	-0.2966746693423019
1105	4	-0.08754332054400749
1105	5	-0.13636788794705268
1105	6	-0.18511068503820383
1105	7	-0.19627248197232205
1105	8	-0.22614073837006288
1105	9	-0.27749502759326106
1105	10-11	-0.270996268008588
1105	12-13	-0.23011614875363762
1105	14-15	-0.32944445523278176
1105	16-17	-0.34742089617638783
1105	18-19	-0.18564884130681492
1105	20-21	-0.12916087654234332
1105	22-23	-0.27694558402946257
1105	24-25	-0.20890359657865787
1105	26-27	-0.45523018370620605
1105	28-29	-0.46611276919363576
1105	30-31	-0.3772353258838095
1105	32-33	-0.2589156083339148
1105	34-35	-0.16667406302720877
1105	36-37	-0.026244175100124778
1105	38-39	-0.35880618216587123
1105	40-41	-0.42585988520916374
1105	42-43	-0.40223288241487865
1105	44-45	-0.47413925340626406
1105	46-47	-0.3975322470529292
1105	48-49	-0.6637184811107559
1105	50-51	-0.3621484813156357
1105	52-53	-0.7308362185996273
1105	54-55	-1.5337661953302515
1105	56-57	-0.46498844127084027
1105	58-59	-0.2760458544981006
1105	60-61	-0.5456864816022815
1105	62-63	-0.09610169740017938
1105	64-65	-0.30117336317464805
1105	66-67	-0.19436778317597003
1105	68-69	-0.0942200867730989
1105	70-71	-0.046267754248006554
1105	72-73	0.02088538917377747
1105	74-75	-0.2838448336510133
1105	76-77	-0.2832336820825212
1105	78-79	0.003236946351098169
1105	80-81	-0.0036086360149880647
1105	82-83	-0.11250409959012231
1105	84-85	-0.03957356810150259
1105	86-87	0.07019160278182213
1105	88-89	-0.19687162179208073
1105	90-91	-0.09822802976667333
1105	92-93	-0.04721021127808811
1105	94-95	-0.17752101590529534
1105	96-97	0.04688347226194267
1105	98-99	-0.07939732002384403
1105	100	-0.10807624963304363
1106	1	-0.1401274808287738
1106	2	-0.23901144048140566
1106	3	-0.16073263418586237
1106	4	-0.019841972903975602
1106	5	-0.0965028648476931
1106	6	-0.1980512937826049
1106	7	-0.29112533444157407
1106	8	-0.14092858619105897
1106	9	-0.20550379896283033
1106	10-11	-0.27278992792641077
1106	12-13	-0.1481418099772327
1106	14-15	-0.14194578584102402
1106	16-17	-0.3465976932071726
1106	18-19	-0.29885876516053855
1106	20-21	-0.24297578361266403
1106	22-23	-0.24781413412775066
1106	24-25	-0.1220432643169076
1106	26-27	-0.04526268603033401
1106	28-29	-0.24032274272340004
1106	30-31	-0.23844046664441976
1106	32-33	-0.13488872649902106
1106	34-35	-0.33487181625083196
1106	36-37	-0.2122738529864776
1106	38-39	-0.3105104252094506
1106	40-41	-0.2527513004795736
1106	42-43	-0.46197896687833406
1106	44-45	-0.4936991946466023
1106	46-47	-0.6135875435834137
1106	48-49	-0.3670460662164601
1106	50-51	-0.43431180194610164
1106	52-53	-0.4956840324989855
1106	54-55	-0.49817863421097996
1106	56-57	-0.3057146340280781
1106	58-59	-0.4544396151873187
1106	60-61	-0.507292224152657
1106	62-63	-0.8210011034166556
1106	64-65	-0.40016352022190205
1106	66-67	-0.5850822665806206
1106	68-69	-0.6472936144107138
1106	70-71	-0.39003305624285645
1106	72-73	-0.445139746903088
1106	74-75	-0.5024282858520159
1106	76-77	-0.5100935175838579
1106	78-79	-0.33037137935341576
1106	80-81	-0.4678454346070282
1106	82-83	-0.20323029234735657
1106	84-85	-0.3841283643232849
1106	86-87	-0.45165166221147146
1106	88-89	-0.488491273633997
1106	90-91	-0.4165077484583435
1106	92-93	-0.1406384258501987
1106	94-95	-0.1879494362781493
1106	96-97	-0.13178705494279086
1106	98-99	-0.42832893030820784
1106	100	-0.40221163813516725
1107	1	-0.10045623562955441
1107	2	-0.13958033105694057
1107	3	-0.0845842236710439
1107	4	-0.14681104057700622
1107	5	-0.06393308878467252
1107	6	0.05679341209147992
1107	7	-0.21685331408941977
1107	8	-0.2008536008613362
1107	9	-0.0844942026678197
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2314	88-89	0.674403693349106
2314	90-91	0.46551441489818046
2314	92-93	0.20191027565425657
2314	94-95	-9.656553261301326E-4
2314	96-97	0.0841366787758453
2314	98-99	-0.15568183296434412
2314	100	0.2431376903311815
2315	1	0.1783210167634337
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2315	4	-0.022730075361209856
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2315	100	0.3752605368907247
2316	1	0.06720490245027122
2316	2	-0.022087326067726565
2316	3	0.03551189451935244
2316	4	0.1118094674461858
2316	5	0.06182962022288052
2316	6	0.16113284709687292
2316	7	0.16730024746667738
2316	8	0.169016022250851
2316	9	0.014892891115891871
2316	10-11	0.12854544972016413
2316	12-13	0.18372720977032486
2316	14-15	0.1076470480351972
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2316	28-29	0.06871051341801859
2316	30-31	0.13643229394190115
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2316	52-53	0.33051382054797784
2316	54-55	0.6022270474529776
2316	56-57	0.33685553850856564
2316	58-59	0.3175839368281501
2316	60-61	-1.3794176737319148
2316	62-63	0.1366361242909946
2316	64-65	0.20158930873439118
2316	66-67	0.24967773867860643
2316	68-69	0.534407449310784
2316	70-71	0.244607450000359
2316	72-73	0.24364908232465154
2316	74-75	0.19794940314092813
2316	76-77	0.12662364192346942
2316	78-79	0.06220621548236238
2316	80-81	0.2566186753956856
2316	82-83	0.129444046855955
2316	84-85	-0.3288009561053151
2316	86-87	0.29757747900731246
2316	88-89	0.2527832835728425
2316	90-91	0.1739478110557684
2316	92-93	0.40102452966690194
2316	94-95	0.5114161692118095
2316	96-97	0.4010863079100879
2316	98-99	0.5813131664276803
2316	100	0.2691172927144514
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	62.0
14	277.0
15	740.0
16	1658.0
17	3466.0
18	5385.0
19	7364.0
20	9843.0
21	12285.0
22	12919.0
23	12528.0
24	12563.0
25	13244.0
26	14735.0
27	16530.0
28	19763.0
29	22840.0
30	27150.0
31	32510.0
32	39782.0
33	50998.0
34	69478.0
35	120004.0
36	182955.0
37	145127.0
38	104642.0
39	5900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.30009805603384	17.860705368091338	13.666745026780097	18.172451549094728
2	13.819900597578986	25.933763477285115	31.553123130911615	28.693212794224287
3	29.190151541613346	26.78455941580745	30.40803084814402	13.617258194435184
4	13.49716434119358	18.96483105655017	35.18185686432659	32.35614773792966
5	9.779254939111198	41.84672216606422	33.95123603473916	14.422786860085418
6	27.278019638912436	31.901959974893014	15.839416908263182	24.98060347793136
7	19.384243779088166	36.48366722871595	22.36956169681728	21.7625272953786
8	26.60795656016639	24.77859339193759	21.597662886145088	27.015787161750932
9	23.203321065628515	20.5469766299694	23.987967276241402	32.26173502816068
10-11	25.38303500265148	24.862689559325837	23.376698981854595	26.377576456168093
12-13	33.046557499207466	23.62894565954296	23.39317062121298	19.931326220036592
14-15	28.33263024399229	24.30871850190628	28.73278247121524	18.625868782886187
16-17	19.706861767551352	28.728319477698673	31.261233225579048	20.303585529170928
18-19	20.68047199488545	30.82182066057268	29.887505345295505	18.610201999246367
20-21	22.869713082674295	24.7727572229464	26.68146423999166	25.676065454387643
22-23	19.461284628961707	27.214361509292168	26.87961598204811	26.444737879698017
24-25	25.860340653722346	27.945682974617686	23.120302144672507	23.073674226987457
26-27	23.990451035088743	22.020632962984525	31.126132086956982	22.86278391496975
28-29	20.109237612715138	28.615988346858796	29.76653490543467	21.508239134991396
30-31	23.312158345577494	25.48715527571591	27.336058805845013	23.864627572861576
32-33	19.960939567477162	26.702833733817442	31.567604187617103	21.768622511088292
34-35	20.692892914721117	26.36954218214268	29.972837276656467	22.96472762647974
36-37	21.975957866725096	25.13494789177494	31.414821271778358	21.474272969721607
38-39	23.350192015378166	25.101988322416474	29.489338594879307	22.058481067326053
40-41	19.82123548424828	28.395009455317606	28.62910332468338	23.154651735750733
42-43	21.97032487665357	27.36787562572973	25.785833976396667	24.87596552122003
44-45	24.318744052275402	23.078600470631073	27.214353083395697	25.38830239369782
46-47	23.640532798079185	27.822629308975884	30.32009383886055	18.216744054084383
48-49	22.701763338316187	25.967634018370223	30.82364287740903	20.506959765904558
50-51	16.322438509512725	27.901272209676925	33.610282697969566	22.166006582840787
52-53	18.73323759985984	30.185473459897004	25.327467232104624	25.75382170813853
54-55	17.59247694030625	26.719871030842935	26.28005910733528	29.407592921515537
56-57	22.913207754878027	23.42023548738967	25.640678231626996	28.025878526105313
58-59	25.518669344125026	22.725068991835304	27.669086894250626	24.087174769789037
60-61	22.18271097383613	26.457546960109575	29.864893758904586	21.49484830714971
62-63	16.848371431353705	27.558702987861416	30.39213299518606	25.20079258559882
64-65	15.298887013035126	31.99453826653471	27.738990531936853	24.967584188493312
66-67	20.687041808062695	26.722685250850493	23.83841794514081	28.751854995946
68-69	20.176034684051032	25.300897479457678	25.90904327779524	28.614024558696045
70-71	22.641213859677904	26.057390540404658	24.208604347158786	27.09279125275865
72-73	22.320057243808026	22.428712511815533	30.04871227894501	25.202517965431433
74-75	20.3856004259515	27.875635711968293	25.548462832715064	26.19030102936514
76-77	19.331361654516567	24.97763866409939	27.80506419423506	27.885935487148977
78-79	20.907278935903108	25.23043718496157	28.9517807279571	24.910503151178226
80-81	21.452765102560765	24.16616124569598	26.97868340747938	27.40239024426387
82-83	18.41144133974138	23.935604093995284	29.04203706908551	28.610917497177823
84-85	19.262391168932066	26.062034039494876	26.380211599720777	28.295363191852285
86-87	19.775687520607228	25.303796175404486	25.95190173944844	28.968614564539852
88-89	19.123191596560382	23.915487173407737	25.644028722760165	31.31729250727172
90-91	21.19715015157417	22.282567276915	26.06668204989246	30.453600521618373
92-93	20.454828116358517	24.68530117676323	27.574841029793518	27.285029677084736
94-95	18.363577473972114	24.06954811612734	28.226759078534563	29.340115331365986
96-97	16.91772419202381	24.58461984658164	29.436128785048115	29.061527176346434
98-99	20.461210252198057	24.234310854630333	24.89771141361212	30.406767479559484
100	18.486254651994802	21.134980883953105	26.75039904483274	33.62836541921935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	551.0
1	366.0
2	227.5
3	281.0
4	239.5
5	237.0
6	356.5
7	619.0
8	973.0
9	1145.0
10	978.5
11	769.5
12	712.5
13	736.0
14	798.5
15	848.5
16	917.5
17	989.5
18	1089.5
19	1196.5
20	1307.5
21	1526.5
22	1711.0
23	1895.0
24	2176.5
25	2507.0
26	3179.5
27	3937.5
28	4498.5
29	5239.0
30	6134.5
31	6881.0
32	7821.0
33	8650.0
34	8965.0
35	9284.0
36	9249.0
37	8655.5
38	8333.5
39	8041.5
40	7439.0
41	7253.5
42	7199.0
43	7520.5
44	8267.5
45	10195.0
46	19294.0
47	59774.0
48	126065.5
49	140601.0
50	146166.5
51	134459.5
52	74858.5
53	35283.5
54	13266.5
55	5697.0
56	3533.0
57	2541.5
58	2014.0
59	1807.5
60	1733.0
61	1527.5
62	1300.0
63	952.0
64	528.5
65	361.5
66	305.0
67	192.5
68	118.0
69	121.5
70	129.0
71	115.0
72	89.0
73	78.5
74	67.5
75	64.5
76	47.0
77	19.5
78	8.5
79	3.5
80	2.5
81	2.5
82	1.0
83	0.5
84	1.0
85	1.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04223308215763418
2	0.011854900254774507
3	0.0
4	3.175419711100315E-4
5	2.1169464740668765E-4
6	0.0
7	0.0
8	2.1169464740668765E-4
9	0.0
10-11	0.0
12-13	5.292366185167191E-5
14-15	0.012331213211439556
16-17	0.0015877098555501573
18-19	1.0584732370334382E-4
20-21	7.938549277750786E-4
22-23	0.0010055495751817665
24-25	0.004657282242947128
26-27	0.016088793202908264
28-29	0.012595831520697915
30-31	0.0094204118095976
32-33	0.007091770688124036
34-35	0.008467785896267505
36-37	0.004286816609985425
38-39	0.004339740271837096
40-41	0.0053452898470188635
42-43	0.006350839422200629
44-45	0.0071976180118273805
46-47	0.01682972446883167
48-49	0.019581754885118607
50-51	0.019052518266601887
52-53	0.01275460250625293
54-55	0.003704656329617034
56-57	0.003757579991468706
58-59	0.008467785896267505
60-61	0.002011099150363533
62-63	1.0584732370334382E-4
64-65	2.1169464740668765E-4
66-67	0.001164320560736782
68-69	0.0
70-71	2.1169464740668765E-4
72-73	0.0027520304162869393
74-75	0.005133595199612175
76-77	0.0048689768903538155
78-79	0.0032812670348036583
80-81	0.0
82-83	7.938549277750786E-4
84-85	1.5877098555501574E-4
86-87	3.7046563296170334E-4
88-89	1.0584732370334382E-4
90-91	5.292366185167191E-4
92-93	4.763129566650472E-4
94-95	1.0584732370334382E-4
96-97	0.0021169464740668762
98-99	3.7046563296170334E-4
100	1.0584732370334382E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	944757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.226634793464154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75047841346074	11.804954442650248
2	10.69816428230282	3.4719040953882083
3	4.510306705453429	2.1956129914771587
4	2.6270278094701003	1.7051128342618178
5	1.7277585828767954	1.4017853767807464
6	1.2815396137463524	1.2477045171371504
7	0.9449858552418993	1.0733758250599776
8	0.6929017499947443	0.8994770915933534
9	0.5614138840526217	0.8198872258051909
>10	3.678877915263729	11.025169405943082
>50	0.25058083737040837	2.770111954951808
>100	0.20176638853201712	6.348371253656379
>500	0.021478357488892146	2.489288145877239
>1k	0.03319382521010605	14.114696798638501
>5k	0.009112030449833032	11.104738709648204
>10k+	0.010413749085523465	27.527809331130946
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	40877	4.326721051021585	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	27576	2.9188457984434093	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	25930	2.744621103627705	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	24980	2.644066146109529	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	17660	1.8692637366010518	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	14016	1.483556089026067	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	12092	1.2799058382208335	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	11581	1.225817855808425	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	11524	1.2197845583573343	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	11458	1.2127986349929134	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	10952	1.1592398891990214	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	10914	1.1552176908982945	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	10585	1.1203939213998944	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	10435	1.104516822844393	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	10043	1.063024671952682	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	10026	1.0612252674497253	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	9803	1.0376213142638795	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	9649	1.0213208264135645	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	9468	1.0021624608232593	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8818	0.9333617004160858	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	8295	0.8780035501192371	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	7866	0.8325950482505026	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	7803	0.8259266668571917	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	7393	0.782529264138821	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7155	0.7573376010974251	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	6592	0.6977455578524425	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	6389	0.6762585511406637	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	5657	0.598778310189816	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5239	0.5545341288818183	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	5019	0.5312477176670827	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4718	0.4993876732323762	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	4563	0.4829813380583579	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4507	0.4770538879309706	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	4281	0.4531323927740149	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	4221	0.4467815533518143	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	4194	0.443923675611824	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	4166	0.44095995054813036	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	4151	0.43937224069258024	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	4127	0.4368319049237	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	3968	0.42000218045486826	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	3927	0.4156624401830312	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	3881	0.4107934632926774	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	3774	0.3994677996564196	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	3752	0.397139158534946	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	3670	0.38845967799127185	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	3637	0.3849667163090615	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	3549	0.3756521518231672	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3538	0.37448783126243046	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	3410	0.36093937382840247	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	3046	0.3224109480003853	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	2993	0.31680103984410807	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	2853	0.30198241452563995	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2675	0.28314159090644475	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	2668	0.28240065964052136	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	2509	0.26557093517168967	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	2508	0.26546508784798634	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	2328	0.2464125695813844	RNA PCR Primer, Index 26 (100% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	2100	0.22227937977702203	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	2055	0.21751625021037155	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	2004	0.21211803670150103	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2000	0.21169464740668767	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	1939	0.2052379606607837	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1851	0.1959233961748894	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	1826	0.19327721308230583	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	1826	0.19327721308230583	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	1590	0.16829724468831667	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	1576	0.16681538215646985	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	1526	0.16152301597130267	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	1525	0.1614171686475993	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	1521	0.16099377935278597	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT	1316	0.13929507799360047	RNA PCR Primer, Index 26 (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1314	0.1390833833461938	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	1270	0.13442610110324665	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1202	0.12722848309141926	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	1191	0.12606416253068248	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	1149	0.12161857493514205	No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1075	0.1137858729810946	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	1065	0.11272739974406117	No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC	1050	0.11113968988851101	No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC	1040	0.11008121665147758	No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA	1021	0.10807011750111405	No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT	964	0.10203682005002344	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	950	0.10055495751817663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0584732370334382E-4	0.0
5	0.0	0.0	0.0	1.0584732370334382E-4	0.0
6	0.0	0.0	0.0	1.0584732370334382E-4	0.0
7	0.0	0.0	0.0	1.0584732370334382E-4	0.0
8	0.0	0.0	0.0	1.0584732370334382E-4	0.0
9	0.0	0.0	0.0	1.0584732370334382E-4	0.0
10-11	0.0	0.0	0.0	1.0584732370334382E-4	0.0
12-13	0.0	0.0	0.0	1.0584732370334382E-4	0.0
14-15	0.0	0.0	0.0	3.175419711100315E-4	0.0
16-17	0.0	0.0	0.0	5.292366185167192E-4	0.0
18-19	0.0	0.0	0.0	8.997022514784226E-4	0.0
20-21	0.0	0.0	0.0	0.0023815647833252363	0.0
22-23	0.0	0.0	0.0	0.006827152378865676	0.0
24-25	0.0	0.0	0.0	0.016829724468831665	0.0
26-27	0.0	0.0	0.0	0.04016905934541898	0.0
28-29	0.0	0.0	0.0	0.1078054991918557	0.0
30-31	0.0	0.0	0.0	0.26403614897799116	0.0
32-33	0.0	0.0	0.0	0.5640603880151193	0.0
34-35	0.0	0.0	0.0	1.0902274341444413	0.0
36-37	0.0	0.0	0.0	1.828618364298968	0.0
38-39	0.0	0.0	0.0	2.7531418131858247	0.0
40-41	0.0	0.0	0.0	3.8583995672961406	0.0
42-43	0.0	0.0	0.0	4.811978106539565	0.0
44-45	0.0	0.0	0.0	5.73390829599569	0.0
46-47	0.0	0.0	0.0	6.831915508432327	0.0
48-49	0.0	0.0	0.0	7.817883328728975	0.0
50-51	0.0	0.0	0.0	8.770615089382773	0.0
52-53	0.0	0.0	0.0	9.719112957088436	0.0
54-55	0.0	0.0	0.0	10.774410774410775	0.0
56-57	0.0	0.0	0.0	12.018857759190988	0.0
58-59	0.0	0.0	0.0	13.000697533863205	0.0
60-61	0.0	0.0	0.0	13.881982351017246	0.0
62-63	0.0	0.0	0.0	14.707062239284811	0.0
64-65	0.0	0.0	0.0	15.621053879463185	0.0
66-67	0.0	0.0	0.0	16.62596837070273	0.0
68-69	0.0	0.0	0.0	17.8405664101986	0.0
70-71	0.0	0.0	0.0	19.2681292649856	0.0
72-73	0.0	0.0	0.0	20.36047364560411	0.0
74-75	0.0	0.0	0.0	21.45604636959557	0.0
76-77	0.0	0.0	0.0	22.511344186917906	0.0
78-79	0.0	0.0	0.0	23.88937049421174	0.0
80-81	0.0	0.0	0.0	25.185629743944737	0.0
82-83	0.0	0.0	0.0	26.493532199285106	0.0
84-85	0.0	0.0	0.0	27.831812836528336	0.0
86-87	0.0	0.0	0.0	29.0018491527451	0.0
88	0.0	0.0	0.0	30.052066298529674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGGCG	30	1.3695349E-4	62.68054	9
GTACCCA	600	0.0	61.90031	1
GCGTCAG	25	0.0052300706	56.41547	1
TACCCAT	710	0.0	52.293507	2
ACCCATG	780	0.0	47.590424	3
TCAGTAC	65	1.4154211E-4	36.144623	3
ACACGTT	55	0.0027201644	34.1731	7
GCTTATG	430	0.0	33.893017	1
CTTATGT	440	0.0	33.1122	2
GCGGTCT	30	0.0041560703	31.34193	12-13
TGGCGGT	30	0.0041560703	31.34193	10-11
TTATGTA	465	0.0	31.32534	3
ATATGTA	80	4.7873904E-4	29.36751	4
GTATAGA	65	0.006145379	28.931011	1
ACGTTTC	65	0.006146969	28.92948	9
GTAGATG	65	0.006146969	28.92948	8
GTATATA	120	5.82929E-6	27.424189	1
TATGTAC	540	0.0	26.9746	4
ATGGCGG	35	0.008825622	26.864511	10-11
TAGATGG	70	0.008825706	26.863089	9
>>END_MODULE