FastQCFastQC Report
Mon 6 Jun 2016
ERR523096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38250536
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA24000.013.7027789
CGACGAT72700.011.2439231
CCGGGAT109500.010.9402951
CGGCAAT69050.010.8177381
CTCGAAT118250.010.2896751
TTGCGCG30850.010.2030118
TCGAATG78750.010.1415472
CGAAAAT73200.010.0119011
CGGCGGT94750.09.5693211
CGGCGCT141550.09.5252211
CGGGAAT100550.09.4845551
TGCGCGG72000.09.46017557
CGGCGAT51550.09.386671
CGAATGT76700.09.3714443
CCGGAAT92600.09.3349051
CCGGTTT87000.09.1797891
CCGAAAT66350.09.0630021
CGAAGAT160950.09.048441
TCGCACG24000.08.80892859
CGCCAAC98200.08.7068871
Produced by FastQC (version 0.11.5)