FastQCFastQC Report
Mon 6 Jun 2016
ERR523096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38250536
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA22850.015.4296059
CGACGAT75200.012.3833251
CCGGGAT110000.010.6890041
CGCGGAT43500.010.5956291
CGACAAT46400.010.541571
CGGCGGT96500.010.3323351
CCGGAAT89050.010.1932651
TTGCGCG31150.010.1107396
CGAAAAT68450.09.6880331
CGCAGAT103500.09.5880891
CGGAAAT85550.09.0159961
CGGGAAT98000.08.97440051
CGGGTAT32300.08.8821331
CGGCAAT63100.08.7951361
CCGGTAT60450.08.7916841
CGGCGCT139000.08.7634461
CGAAGAT157350.08.7577151
CTCGAAT116000.08.7170681
CGGCGAT49100.08.7166551
TGCGCGG73200.08.66949757
Produced by FastQC (version 0.11.5)