FastQCFastQC Report
Mon 6 Jun 2016
ERR523095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8102280
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT511850.6317357583297541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393030.48508567958648674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230130.28403116159895736No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT95790.11822598083502421No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT87500.1079942929644495No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG85360.10535306111366183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA368800.031.9345661
CGTCGAC753.2702187E-431.34485494
GTATCAA565000.030.4473021
GTACATG441600.027.2134081
TCAACGC655200.025.8787464
ACATGGG446450.025.8122023
ATCAACG658250.025.767573
CAACGCA660500.025.6129025
AACGCAG675900.025.0937276
TACATGG483850.024.5872462
TATCAAC692150.024.5695292
GAGTACT349700.023.61101212-13
CATGGGG163100.022.9932044
ACGCAGA742850.022.7815347
GTACTTT358550.022.6687414-15
CGCAGAG747450.022.6087848
CATGGGA223500.022.540724
CGCCGTA10250.021.55914594
AGAGTAC585400.021.21749310-11
GCAGAGT787700.021.1663159
Produced by FastQC (version 0.11.5)