FastQCFastQC Report
Mon 6 Jun 2016
ERR523095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8102280
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453760.5600398900062699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367160.4531563954837404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213760.2638269721609226No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT158440.19554989459757005No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT96230.11876903785107402No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG90930.11222766924865593No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT82660.10202066578790167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA365700.032.1347541
GTATCAA557350.030.1840171
GTACATG433850.025.5038191
TCAACGC656900.025.4178944
ATCAACG661750.025.2468973
CAACGCA662650.025.1618735
AACGCAG674700.024.7118786
TATCAAC692050.024.1948782
ACATGGG440450.023.8155123
TACATGG473150.023.141692
GAGTACT355100.023.11222312-13
ACGCAGA741850.022.4366117
GTACTTT363700.022.35609614-15
CGCAGAG747200.022.256448
CATGGGA215950.021.783344
AGAGTAC591800.021.58830810-11
CATGGGT53350.020.9645794
GCAGAGT794750.020.6999639
CATGGGG161700.020.6344034
AGTACTT405300.019.8958412-13
Produced by FastQC (version 0.11.5)