FastQCFastQC Report
Mon 6 Jun 2016
ERR523094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398893
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT994140.872137320702984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401370.35211313940748457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331620.29092298699531616No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT289530.25399834878702693No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC236590.2075552424257338No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC208740.18312304536940563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188910.16572661924276333No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA176280.15464659594576421No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA145340.12750360934171415No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC127180.11157223776028075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG419550.026.4147971
TACATGG436650.024.9687392
ACATGGG447200.023.749963
CATGGGG301150.021.4957054
GAGTACT339850.021.26259612-13
AGAGTAC540200.019.7372910-11
GTACTTT364550.019.71850214-15
AGTACTT357350.018.51869212-13
GAGTACA334300.017.0887891
ATGGGGG162550.017.0345555
ACTTTTT444100.016.33407816-17
AGTACAT329950.015.9872612
TACTTTT410700.015.90119214-15
CATGGGA144550.015.773364
TATACCG7800.015.0677345
TATACTG50250.015.0623375
TACCTGG74600.014.9361552
GTATATA52300.014.6540381
GTATAGA34500.014.1737781
ATGGGGA126550.014.0049545
Produced by FastQC (version 0.11.5)