FastQCFastQC Report
Mon 6 Jun 2016
ERR523094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398893
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1004910.8815856066023253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377650.3313041011964934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322000.2824835709923762No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT291060.25534058438832613No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC246170.21595956730184238No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC213350.18716729773671884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181510.15923476077896334No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA175970.15437463971282123No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG174920.15345349763349828No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA150480.13201281913954277No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC132730.11644113160813072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG426600.025.6531851
TACATGG440650.024.3510022
ACATGGG449050.023.1386363
GAGTACT346950.021.06437312-13
CATGGGG300800.020.5599374
AGAGTAC555000.019.56355710-11
GTACTTT366400.019.5062914-15
AGTACTT365700.018.5380912-13
ATGGGGG160050.017.6171445
GTATAGG28500.016.6664181
GAGTACA345000.016.2897571
ACTTTTT443600.016.26743716-17
TACTTTT413850.016.21918314-15
GTATATA47300.015.31176951
CATGGGA148950.015.2703344
AGTACAT339750.015.148142
GTATAGA36400.014.4704631
TACCTGG77450.014.3824062
TATACCG7200.014.3591945
ATGGGGA122250.014.1077295
Produced by FastQC (version 0.11.5)