FastQCFastQC Report
Mon 6 Jun 2016
ERR523093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70580891
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAAT122900.09.9423441
CTCGAAT214000.09.8385611
CCGGGAT213250.09.7409331
CGCGAAT49850.09.710451
CGACGAT142250.09.6801621
CGCGCTA45300.09.6461539
GTCGGTT129100.09.5012671
CGGCAAT125900.09.257491
CGACAAT86450.09.1329611
CTCGGAT287900.09.0924471
CCGGTAT116250.08.9748531
CTCGTAT120600.08.9239171
CGGGAAT179500.08.8233351
CGCGGAT85950.08.8033361
CTCGATT119800.08.7089031
GCGTCGT463400.08.63521394
CCCGAAT176350.08.4745951
CCGACAT182750.08.4349741
CGCATAT41250.08.43092251
AGCGTCG537050.08.30028794
Produced by FastQC (version 0.11.5)