FastQCFastQC Report
Mon 6 Jun 2016
ERR523093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70580891
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT51700.011.3750291
CGCGTAA15000.010.3503671
CCGGTAT121750.09.7765261
CCGAAAT120250.09.5463591
CCGGGAT218900.09.5211841
CCGGGTT206700.09.1499481
CTCGGAT295850.09.0802451
GTCGGTT130250.09.06627751
CGACGAT145650.09.0444161
CCGGAAT198300.08.9918591
CTCGAAT218650.08.8865591
CGGGAAT171700.08.8230471
CTCGGTT191150.08.7374971
GTTCGAT118250.08.673381
CCCGAAT177100.08.6071511
CGCGGAT85700.08.3993111
CCGACAT183100.08.35081
CGGCGAT101150.08.3256881
CGGCAAT120550.08.3127641
GTCGTTT143200.08.2463871
Produced by FastQC (version 0.11.5)