FastQCFastQC Report
Mon 6 Jun 2016
ERR523092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782542
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345500.35318018568179926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282420.2886979682785926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171740.1755576413574304No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129090.13195956633766562No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112120.11461233695699953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG164650.053.6982732
GTACCTG194300.045.974931
ACCTGGG189750.045.85253
CCTGGGG163250.033.4426654
TATAACG8500.027.1107372
CTGGGGG106800.025.0072255
CATGGGG254600.024.6941954
ATGGGGG162050.023.9963345
TGGGGGG172350.023.4218316
GTACATG515650.023.2806241
GTATCAA525300.022.6290721
TACATGG523550.022.5195852
GTACACG18750.022.3284021
GTACCCG18800.021.7685931
ACATGGG531300.021.4125883
GGTATCA402500.021.118411
TATCACG9400.020.5125772
GAGTACT288150.020.33454712-13
TCAACGC591550.019.762034
CAACGCA592400.019.7321555
Produced by FastQC (version 0.11.5)