FastQCFastQC Report
Mon 6 Jun 2016
ERR523092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782542
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308830.3156950412275255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274290.28038724495126116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165470.1691482643263888No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA127250.13007866462520684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98510.10069979765995382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG168750.052.3022
GTACCTG195950.045.3743441
ACCTGGG191750.045.0691683
CCTGGGG157400.034.9014134
TATAACG8400.026.2957212
CTGGGGG102250.026.1965895
TGGGGGG155550.023.8363726
CATGGGG242150.022.5310254
GTATCAA504350.022.0663431
GTACATG496400.021.6335811
ATGGGGG155300.021.5750245
GTACCCG15650.021.3308491
GGTATCA389150.021.0109981
TACATGG508600.020.8740352
ACATGGG506650.020.2756753
GAGTACT290350.019.38687912-13
TAACGCA12950.019.2326564
TCAACGC569550.019.1750934
CAACGCA573250.019.0595265
ATCAACG577500.018.8704383
Produced by FastQC (version 0.11.5)