##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523091_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019047 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.089809202064142 29.0 27.0 30.0 17.0 30.0 2 30.045528410185597 34.0 28.0 34.0 14.0 35.0 3 28.842019031751118 34.0 27.0 34.0 11.0 34.0 4 29.264177604582756 34.0 27.0 35.0 13.0 35.0 5 29.25737637608238 35.0 27.0 35.0 11.0 36.0 6 29.66260914183771 34.0 26.0 35.0 12.0 36.0 7 29.84084174365431 35.0 29.0 36.0 12.0 36.0 8 30.131082634530053 35.0 26.0 36.0 11.0 37.0 9 30.389821534615095 35.0 26.0 36.0 12.0 37.0 10-11 30.316176394110688 36.0 26.0 36.5 11.0 37.0 12-13 30.53066347638267 35.0 26.0 36.5 11.0 38.0 14-15 30.492277049518904 35.5 25.0 37.0 11.0 38.0 16-17 30.48578661120816 36.0 25.5 36.5 11.5 38.0 18-19 30.534737428103455 36.0 25.0 37.0 11.0 37.5 20-21 30.820680499265247 36.0 26.5 37.0 11.0 38.0 22-23 30.790453367355987 36.5 23.5 38.0 11.0 38.0 24-25 30.637579759163607 36.5 24.5 37.0 11.0 38.0 26-27 30.767134940395145 36.0 25.0 37.5 11.0 38.0 28-29 30.693555177269275 36.0 25.5 37.5 11.0 38.0 30-31 30.706420900553578 35.5 26.5 38.0 11.0 38.0 32-33 30.598988532708745 36.0 25.5 37.5 11.0 38.0 34-35 30.254327908166577 36.0 24.5 37.0 11.0 37.5 36-37 29.950818876430315 36.0 24.0 37.0 10.5 37.5 38-39 29.90923440613319 36.0 24.5 37.5 10.5 38.0 40-41 29.81200957679539 36.0 23.0 37.5 10.5 39.0 42-43 29.12218883463337 35.5 23.0 37.0 9.0 38.0 44-45 28.863779050215275 35.5 22.0 37.0 10.0 38.0 46-47 28.239020438850606 35.0 20.5 37.0 10.0 37.5 48-49 27.862811514541267 34.5 19.0 37.0 8.0 38.0 50-51 27.705795357908954 34.5 20.5 37.0 6.5 38.0 52-53 27.61582345532323 34.5 19.0 37.0 7.0 37.5 54-55 27.608444974287373 34.0 12.0 37.5 7.0 38.5 56-57 27.439739144259644 34.5 11.5 37.0 7.0 38.0 58-59 27.437517056314192 34.5 12.0 37.0 7.0 38.5 60-61 27.08319717173498 34.5 12.0 37.0 7.5 38.0 62-63 26.91020615171415 34.5 12.0 36.5 7.0 37.5 64-65 26.735957360081265 33.5 11.5 36.5 7.0 38.0 66-67 26.84724699325969 33.5 11.0 37.0 7.0 38.0 68-69 26.637860089438234 33.0 11.5 37.5 7.0 38.0 70-71 26.132978083224412 32.0 10.5 36.5 7.0 38.0 72-73 26.135814569943147 32.5 11.0 37.0 7.0 38.0 74-75 25.73550962409493 32.5 11.0 36.5 6.5 38.0 76-77 25.399561773450543 31.5 11.0 37.0 6.5 37.5 78-79 25.285246703023752 31.5 11.0 36.0 7.0 37.5 80-81 25.08174079157147 31.0 10.5 37.0 6.5 37.0 82-83 25.035844633631612 30.5 11.0 37.0 7.0 38.0 84-85 24.625408422884657 29.5 11.0 36.5 6.5 37.0 86-87 24.536933513682445 29.0 11.0 37.0 6.5 38.0 88-89 24.30845790117813 29.0 10.5 36.5 6.5 37.5 90-91 24.099875089584344 29.0 10.5 36.0 6.0 38.0 92-93 24.004456062686998 27.5 10.5 37.0 7.0 37.0 94-95 23.795328191963833 26.5 11.0 36.0 6.0 37.5 96-97 23.73124102608805 27.0 10.5 36.0 7.0 37.0 98-99 23.820573270458787 24.0 11.5 36.0 7.0 37.5 100 23.352697584553503 21.0 11.0 35.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3738757682343419 1101 2 0.20797284800998384 1101 3 0.1512723788206074 1101 4 -0.061099556332198546 1101 5 0.12688994746090998 1101 6 0.10140051384527737 1101 7 -0.10326917639893551 1101 8 0.09157021838850099 1101 9 0.10167514074991502 1101 10-11 0.2781596729823974 1101 12-13 0.1571353376921678 1101 14-15 0.07443759229730773 1101 16-17 0.011872197993771039 1101 18-19 0.05735242412640318 1101 20-21 0.11527195658602452 1101 22-23 -0.06076618641280618 1101 24-25 -0.12936882360877888 1101 26-27 0.14851719806409136 1101 28-29 -0.1330655145842634 1101 30-31 -0.09488100170266023 1101 32-33 0.008033626766778212 1101 34-35 -0.4781312264882054 1101 36-37 -0.1280819180529491 1101 38-39 -0.2056291939394228 1101 40-41 -0.14284237496089247 1101 42-43 -0.18826566978617265 1101 44-45 -0.654479048032691 1101 46-47 -0.27395412056415935 1101 48-49 -0.2627008727508269 1101 50-51 0.041662754901700794 1101 52-53 -0.20890580645362178 1101 54-55 -0.023594534414407065 1101 56-57 -0.36538198297619573 1101 58-59 -0.7467124428837693 1101 60-61 -0.22286398985798783 1101 62-63 -0.36630589170889394 1101 64-65 -0.20377368418239783 1101 66-67 -0.0943174165780718 1101 68-69 -0.6383034326547694 1101 70-71 -0.4565076534238628 1101 72-73 -0.47057036955528275 1101 74-75 -0.22567689848859018 1101 76-77 -0.7892554361567612 1101 78-79 -0.7945166350389883 1101 80-81 -0.21943712809919802 1101 82-83 -1.1607156411863642 1101 84-85 -1.049840452197678 1101 86-87 -0.6499077508691222 1101 88-89 -1.2150487039940536 1101 90-91 -1.1368620549842312 1101 92-93 -1.038344774908012 1101 94-95 -1.4143937008200602 1101 96-97 -1.017413356278162 1101 98-99 -1.6556887650618712 1101 100 -1.861663148093399 1102 1 0.27512529448754464 1102 2 0.12458231362359484 1102 3 -0.03156281056435617 1102 4 -0.0018913335860268887 1102 5 0.15094685145691855 1102 6 0.19904451483744268 1102 7 0.10758435579614911 1102 8 -0.07288858936213671 1102 9 -1.6584078280596515 1102 10-11 0.03877617918092113 1102 12-13 -0.13846130027316406 1102 14-15 0.05883973909860529 1102 16-17 0.03571088713985304 1102 18-19 0.03197346830596359 1102 20-21 -0.023113297916580677 1102 22-23 0.1062034100020881 1102 24-25 -0.10498244709618731 1102 26-27 0.14497554191742523 1102 28-29 -0.08110721318086078 1102 30-31 -0.023416540064918223 1102 32-33 0.03512916907993002 1102 34-35 -0.09132300085654421 1102 36-37 0.009854837651296577 1102 38-39 -0.14898586271286263 1102 40-41 -0.07307230377263352 1102 42-43 -0.10376450088704914 1102 44-45 -0.16349091903150637 1102 46-47 -0.5226424731349901 1102 48-49 -0.16233646921122613 1102 50-51 -0.011630777226791622 1102 52-53 -0.19149037476555364 1102 54-55 -0.1373452328961413 1102 56-57 -0.23450426264301072 1102 58-59 -0.09283462766249784 1102 60-61 -0.010546351161849543 1102 62-63 -0.0393350079234942 1102 64-65 0.11429256323800274 1102 66-67 0.07210463119558241 1102 68-69 0.00957950874268576 1102 70-71 0.04723654161720958 1102 72-73 -0.0706380397420574 1102 74-75 -0.17275709223785185 1102 76-77 -0.3707500122761509 1102 78-79 -0.3713063436215869 1102 80-81 -0.5702389324155526 1102 82-83 -0.6571351419640585 1102 84-85 -0.7624686967736594 1102 86-87 -0.5805492775573633 1102 88-89 -0.7090148483693248 1102 90-91 -0.6221532920418547 1102 92-93 -0.6981474076916818 1102 94-95 -0.6898486780885378 1102 96-97 -0.6663741930246019 1102 98-99 -1.3891052688188275 1102 100 -0.6833562289763364 1103 1 0.15647196891220005 1103 2 0.14156811329614527 1103 3 0.1616683427404979 1103 4 -0.041446862618538916 1103 5 0.07671686240319886 1103 6 -0.004624561558319584 1103 7 0.2806555088357712 1103 8 -0.22851642021363006 1103 9 0.02157174919617333 1103 10-11 -0.019597726121865833 1103 12-13 -0.12382859194432427 1103 14-15 -0.0620319904856963 1103 16-17 -0.14191076384230072 1103 18-19 -0.10706668482041337 1103 20-21 0.014636591985155434 1103 22-23 -0.1434429494061611 1103 24-25 -0.09976224228302755 1103 26-27 -0.004510822751228716 1103 28-29 -0.5505392663905084 1103 30-31 -0.11539389933115629 1103 32-33 -0.040284687111022066 1103 34-35 -0.16113800814762058 1103 36-37 -0.01524148796769964 1103 38-39 0.09643145252512397 1103 40-41 0.10064907827690206 1103 42-43 0.1386423106728003 1103 44-45 0.1322714168088055 1103 46-47 0.196293378119357 1103 48-49 0.033291980511307884 1103 50-51 0.01564485853887021 1103 52-53 0.23218776919600614 1103 54-55 -0.30147660042983304 1103 56-57 -0.06209521872692747 1103 58-59 0.22593382450236277 1103 60-61 0.22403505717865357 1103 62-63 0.3160761351810386 1103 64-65 0.3187951815597927 1103 66-67 0.3877644996744358 1103 68-69 0.3257274288663261 1103 70-71 0.05927579351264001 1103 72-73 0.16124174787466572 1103 74-75 0.07628628233328882 1103 76-77 -0.5165901490466496 1103 78-79 -0.4864207767440085 1103 80-81 0.13931218163519432 1103 82-83 -0.14888150209064221 1103 84-85 -0.26201020727208757 1103 86-87 -0.36835331867471766 1103 88-89 -0.36093235396541345 1103 90-91 -0.2433293459322634 1103 92-93 -0.2445118995239639 1103 94-95 -0.5782566986571602 1103 96-97 -0.7537431733295854 1103 98-99 -0.4742822300183782 1103 100 -0.4839968710108309 1104 1 0.21640869526398276 1104 2 0.43183301842004695 1104 3 0.5425130088214516 1104 4 0.23914138157209663 1104 5 0.58285022653466 1104 6 0.676844454015356 1104 7 0.4289600307979988 1104 8 0.2620487666306204 1104 9 0.36781142185609994 1104 10-11 0.30671115982470454 1104 12-13 0.1045758039380793 1104 14-15 0.30996385176741015 1104 16-17 0.35124476531486337 1104 18-19 0.34393432299323834 1104 20-21 0.24983087453320607 1104 22-23 0.2475839343322903 1104 24-25 0.3195443324292384 1104 26-27 0.3152009397952291 1104 28-29 0.26649168435036685 1104 30-31 0.4292119602871409 1104 32-33 -0.32161098637255137 1104 34-35 0.4009342382043819 1104 36-37 0.3538250951040567 1104 38-39 0.2360624524879995 1104 40-41 0.23113715981493854 1104 42-43 0.47721462508316037 1104 44-45 0.3474099327516491 1104 46-47 0.4782652005846657 1104 48-49 0.2946209471104666 1104 50-51 0.39376512796746965 1104 52-53 0.2202900684685396 1104 54-55 0.3267479564002649 1104 56-57 0.3898789548452548 1104 58-59 0.5703454084471886 1104 60-61 0.36981688271002255 1104 62-63 0.3689580284957863 1104 64-65 0.3667614741117333 1104 66-67 0.22835656613239053 1104 68-69 0.33771944885210914 1104 70-71 0.30924602657470146 1104 72-73 0.23226703671020843 1104 74-75 0.3924871961026639 1104 76-77 0.28874672607107854 1104 78-79 0.034581069768588435 1104 80-81 0.14651316827513483 1104 82-83 -0.028381912503153472 1104 84-85 -0.1157574729072941 1104 86-87 0.4425285938888557 1104 88-89 0.013620145151204355 1104 90-91 -0.16952218894294546 1104 92-93 0.006023836613099576 1104 94-95 -0.15905558002525666 1104 96-97 -0.35886033682225715 1104 98-99 -0.3598803942301281 1104 100 -0.2692011217020678 1105 1 0.08583649946409366 1105 2 0.36609454775326 1105 3 0.09766200113721979 1105 4 0.05229364190943997 1105 5 0.1329156002399614 1105 6 0.08704089370363377 1105 7 0.267190042704641 1105 8 0.12129924548471394 1105 9 0.24447878318752103 1105 10-11 -0.05633059572144106 1105 12-13 0.016703323148657745 1105 14-15 0.08448868116526853 1105 16-17 -0.08310569880525875 1105 18-19 -0.11903442638455175 1105 20-21 0.04320164896936873 1105 22-23 -0.0717118614415817 1105 24-25 0.04468686038064362 1105 26-27 0.07037687802404591 1105 28-29 0.008045202517617867 1105 30-31 -0.08042392476664162 1105 32-33 0.2132006112546172 1105 34-35 0.22088562937010536 1105 36-37 0.2843216299658309 1105 38-39 -0.06222548649477133 1105 40-41 -0.04690563772833656 1105 42-43 0.16523378190173688 1105 44-45 0.012715543209317559 1105 46-47 0.36941141070172634 1105 48-49 0.038956000292138526 1105 50-51 0.18420171812809372 1105 52-53 0.19986320803907986 1105 54-55 0.27577943002313887 1105 56-57 0.27909939497953573 1105 58-59 0.09582695049675038 1105 60-61 -0.03728582104023559 1105 62-63 -0.07346758153711974 1105 64-65 0.07566384628125178 1105 66-67 -0.06343342565109822 1105 68-69 -0.22582312900896895 1105 70-71 -0.13826094551437862 1105 72-73 -0.03350554774797487 1105 74-75 -0.13298916914587267 1105 76-77 -0.07762955303017449 1105 78-79 -0.31373202315764814 1105 80-81 0.14457757564424512 1105 82-83 -0.29760237918513255 1105 84-85 -0.35787220940148146 1105 86-87 -0.25914377832048174 1105 88-89 -0.4227578993719412 1105 90-91 -0.8400766774285451 1105 92-93 -0.4589639979044797 1105 94-95 -0.7600573573936096 1105 96-97 -0.15277587001285298 1105 98-99 -0.3479030022493923 1105 100 -0.6233365229240597 1106 1 0.07226545308173371 1106 2 -0.13759575700789028 1106 3 0.2219406513377784 1106 4 -0.018770952146777375 1106 5 0.020282943441124246 1106 6 0.10536827018859185 1106 7 0.12103966131073207 1106 8 0.11377935498060054 1106 9 -0.1484880145862384 1106 10-11 0.03814590016399677 1106 12-13 -0.16735354453236084 1106 14-15 -0.2946133746015036 1106 16-17 -0.10184373615924258 1106 18-19 -0.26723144305261215 1106 20-21 0.1811021330926188 1106 22-23 -0.07175816018415304 1106 24-25 -0.036610012286786286 1106 26-27 -0.14863336002587957 1106 28-29 -0.03463095201398758 1106 30-31 0.07471957055500411 1106 32-33 0.05077205367718918 1106 34-35 -0.030238878593532803 1106 36-37 -0.07984516965475308 1106 38-39 -0.0883046319507983 1106 40-41 -0.05722717928921739 1106 42-43 0.007694294589132511 1106 44-45 -0.01790228978380881 1106 46-47 0.07670178256399396 1106 48-49 -0.05550933772459388 1106 50-51 0.13162071071875658 1106 52-53 -0.0811346337469594 1106 54-55 0.05741442458784718 1106 56-57 -0.045428498922728267 1106 58-59 -0.2383541671061593 1106 60-61 -0.21115375907123024 1106 62-63 -0.13839496304953158 1106 64-65 -0.16498060143502613 1106 66-67 -0.11830872171937301 1106 68-69 -0.10237323912559049 1106 70-71 0.07029744758836998 1106 72-73 -0.33107615910170907 1106 74-75 -0.21019661880592722 1106 76-77 -0.5690538972937738 1106 78-79 -0.36775470415495093 1106 80-81 -0.602439810623725 1106 82-83 -0.44978313973624395 1106 84-85 -0.5446274857908655 1106 86-87 -0.621486971789718 1106 88-89 -0.34550453572340345 1106 90-91 -0.7189449309578926 1106 92-93 -0.600121681931082 1106 94-95 -0.4194076257211137 1106 96-97 -0.4645260804733411 1106 98-99 -0.38120706007126515 1106 100 -0.4339933905024722 1107 1 -0.06271719415361687 1107 2 -0.029775829009970778 1107 3 0.13355794519715758 1107 4 0.17379311745860804 1107 5 0.18345307947991785 1107 6 0.05593996509166743 1107 7 0.4452210855025811 1107 8 0.21606957579440333 1107 9 0.15282966195498915 1107 10-11 0.1040778028346736 1107 12-13 0.04907981204595302 1107 14-15 0.10430588570211086 1107 16-17 0.18692949574636586 1107 18-19 -0.12791490615635226 1107 20-21 0.055625805016564556 1107 22-23 0.08076297537185795 1107 24-25 0.058159918089611295 1107 26-27 0.20801934278487622 1107 28-29 -0.09022533354405127 1107 30-31 0.08627112734444609 1107 32-33 0.09069302749673014 1107 34-35 0.09379847058821866 1107 36-37 -0.32691281051882015 1107 38-39 0.037955075526831195 1107 40-41 0.21236059513395134 1107 42-43 0.01927697400983419 1107 44-45 -0.11849417644277693 1107 46-47 -0.018437570915214252 1107 48-49 0.13037298128086405 1107 50-51 0.05291385351748801 1107 52-53 0.3315643613209893 1107 54-55 0.06567963393941412 1107 56-57 0.15057095250926622 1107 58-59 0.07695806626398394 1107 60-61 0.3468208711767282 1107 62-63 0.08880176335571477 1107 64-65 0.2812690961946913 1107 66-67 0.1563894899691789 1107 68-69 -0.06432873224470725 1107 70-71 0.09531624768188962 1107 72-73 -0.12017200364846659 1107 74-75 -0.2288995489890091 1107 76-77 -0.1664487559363046 1107 78-79 0.1615782492967277 1107 80-81 0.23481868534854655 1107 82-83 -0.10403170784217508 1107 84-85 0.03151188084587275 1107 86-87 -0.22559090822060313 1107 88-89 0.05131888104557092 1107 90-91 0.05197428481252686 1107 92-93 -0.1746080066975111 1107 94-95 0.08939198198191534 1107 96-97 -0.18045894465620194 1107 98-99 -0.13085965053690884 1107 100 -0.2107484791792018 1108 1 -0.01043479629926125 1108 2 -0.2589626552743738 1108 3 -0.057686615700415445 1108 4 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0.8037690919488405 2316 100 0.6923868445274337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 112.0 1 62.0 2 41.0 3 43.0 4 62.0 5 60.0 6 89.0 7 210.0 8 267.0 9 323.0 10 412.0 11 452.0 12 864.0 13 3871.0 14 14190.0 15 35156.0 16 61438.0 17 88129.0 18 108052.0 19 115210.0 20 113400.0 21 109475.0 22 99261.0 23 75382.0 24 54846.0 25 44480.0 26 37561.0 27 35853.0 28 37468.0 29 38942.0 30 43274.0 31 51719.0 32 64077.0 33 83941.0 34 116179.0 35 185197.0 36 317992.0 37 73704.0 38 7253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.38137284236349 16.0329904629048 14.631158341936468 17.954478352795242 2 16.90965104103951 22.903216055650287 35.06472398094974 25.122408922360464 3 27.234525820702565 25.516086880656086 30.36321888309896 16.88616841554239 4 13.528568602475957 16.31053732007812 37.153933453880356 33.006960623565575 5 11.37927803171496 36.736025480650284 32.81912913015648 19.065567357478272 6 26.420138446616477 31.604377914558007 16.572259231302596 25.403224407522924 7 21.746844614844026 34.44698799300802 21.966793557746016 21.839373834401936 8 26.44746141044537 22.784464464305714 21.21892739226494 29.54914673298397 9 25.956754270852745 18.08922702421967 23.400338179381762 32.55368052554582 10-11 25.496026855420244 23.061182983144512 24.447727081866077 26.995063079569164 12-13 31.534452590295615 22.83830709762577 25.09020705072925 20.537033261349364 14-15 26.838729880936867 23.328921752495944 30.339420852424446 19.492927514142742 16-17 19.305253106331442 27.109628246165602 30.43185248081593 23.153266166687025 18-19 19.213480946683223 27.918690915614246 31.750588908086236 21.117239229616295 20-21 23.301567478901227 23.14670274395532 29.87018618457894 23.68154359256451 22-23 20.939045260707015 23.994921615320784 30.91018201757266 24.155851106399542 24-25 23.691273721116744 24.544073155424805 29.23902887898003 22.525624244478422 26-27 22.33127891923365 21.507324657305148 34.38851345073432 21.77288297272688 28-29 19.927520253151386 27.447015258555034 31.81735520031158 20.808109287982 30-31 21.455109432989946 23.967137894981988 30.965894169354797 23.611858502673265 32-33 20.191019733932905 24.847354898900104 33.18022786711063 21.78139750005636 34-35 21.557572142300824 23.68445955453207 32.03604191893268 22.721926384234422 36-37 20.629859468650203 22.85943099814613 34.64006614871968 21.870643384483984 38-39 20.55070560378535 23.867740688162783 33.56004239741058 22.02151131064129 40-41 19.388739949037305 25.40869001689013 32.57027189188734 22.632298142185235 42-43 19.049652050344605 25.550989460538652 30.49684262169566 24.902515867421084 44-45 20.157711766902583 22.996890319648575 31.728478244008183 25.11691966944066 46-47 21.390360415621107 25.207259118818815 31.60690719158349 21.795473273976594 48-49 20.518584117735557 24.24804832001975 31.529288769818876 23.70407879242582 50-51 16.94107086163768 24.860670610129763 33.81485319328066 24.383405334951895 52-53 19.00512625312678 25.967572223766922 29.22836582901024 25.79893569409606 54-55 18.136763331745005 23.08466935121579 30.50048232184508 28.278084995194124 56-57 20.976265293941736 21.647993375877103 29.60375426426673 27.771987065914434 58-59 22.938901533580324 21.483674806060602 29.167331379984486 26.41009228037459 60-61 20.128021385446818 22.35231988601826 31.61038834937027 25.909270379164646 62-63 19.161051765418087 22.43834424773369 31.03896438449272 27.36163960235551 64-65 19.322750316197485 24.74861494849932 29.167552217791577 26.76108251751162 66-67 21.086202381781117 22.521310849166333 27.498991096129156 28.893495672923397 68-69 20.89636986851141 22.377313486672275 28.618586970847364 28.107729673968944 70-71 23.37804447232284 22.320440729746828 27.907029171739033 26.394485626191294 72-73 22.711888254976117 20.33936008747451 31.014560176256982 25.93419148129239 74-75 22.20119785226026 23.62479023462751 28.501703519053372 25.672308394058856 76-77 23.07640090961545 21.03950247136641 28.743065346589596 27.14103127242854 78-79 23.80590624700441 21.105508469643315 29.59142550634935 25.497159777002924 80-81 25.5393059582178 20.2540518382658 28.707288045544555 25.499354157971844 82-83 24.36396771893289 19.572260752120233 30.21692355971509 25.846847969231785 84-85 24.716632928215986 20.87049908716706 28.998885677550902 25.413982307066053 86-87 25.842736704131543 20.566545350427578 27.804924081991743 25.785793863449136 88-89 26.288076333972025 20.133199056029895 26.630201098920697 26.948523511077383 90-91 28.19804136438668 18.112747380790548 26.769415441198323 26.919795813624447 92-93 29.095697045442442 18.888111290440452 27.186314377409953 24.829877286707156 94-95 28.404753022288215 18.357518079726677 27.265545691409276 25.97218320657583 96-97 27.459742684124922 18.222916263098522 28.357336039035836 25.960005013740723 98-99 28.737280229566498 19.304448594019163 26.154582833146634 25.803688343267705 100 29.298238051992897 16.434030869142795 26.608917697314343 27.658813381549958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12264.0 1 6990.5 2 1805.0 3 1671.0 4 1162.5 5 1129.0 6 1937.0 7 3399.0 8 4425.0 9 3964.5 10 2476.5 11 1387.5 12 1186.0 13 1173.0 14 1239.5 15 1302.0 16 1385.5 17 1578.5 18 1825.5 19 2177.5 20 2744.0 21 3484.5 22 4330.5 23 5449.0 24 7026.5 25 9050.0 26 11611.5 27 14356.5 28 17294.0 29 20713.5 30 23836.5 31 26435.5 32 28604.5 33 30085.5 34 30482.5 35 29799.0 36 28494.5 37 26456.5 38 24290.5 39 22130.5 40 20331.0 41 19335.5 42 18614.5 43 18637.0 44 19471.0 45 23119.0 46 40768.0 47 84962.5 48 152733.0 49 182362.0 50 169634.0 51 142771.5 52 109974.0 53 87619.5 54 62994.0 55 44269.5 56 35321.0 57 31395.0 58 29442.0 59 28163.0 60 27264.0 61 26465.5 62 25179.0 63 23843.5 64 22559.0 65 21102.0 66 19427.0 67 17599.0 68 15915.5 69 14292.5 70 12546.0 71 10805.5 72 9232.5 73 7761.5 74 6493.5 75 5308.0 76 4228.5 77 3341.5 78 2558.0 79 1880.0 80 1376.0 81 1002.0 82 696.0 83 465.5 84 300.5 85 177.5 86 108.5 87 77.0 88 50.5 89 27.5 90 11.5 91 2.5 92 2.0 93 3.5 94 2.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019959911780161634 2 0.050320770145519146 3 0.08672408319370475 4 0.10549531536412972 5 0.10618871180314278 6 0.06805190765742451 7 0.06468398209650394 8 0.06304954763311602 9 0.0585919990966035 10-11 0.03385260471895899 12-13 0.03831015325547152 14-15 0.043287749121243835 16-17 0.05108845906014075 18-19 0.05534789432836383 20-21 0.0573042628527221 22-23 0.05361440323083117 24-25 0.048909213108956844 26-27 0.051237044011357834 28-29 0.04784435429190108 30-31 0.04541746675535537 32-33 0.03573468076770872 34-35 0.033431614023843925 36-37 0.027983499145884175 38-39 0.02612618725567062 40-41 0.03506604848723185 42-43 0.0571804420600412 44-45 0.06515450110869138 46-47 0.07847761840115658 48-49 0.08650120576687913 50-51 0.08001299623039979 52-53 0.06904247399887174 54-55 0.059607329596586905 56-57 0.04796817508458198 58-59 0.03135142470680474 60-61 0.02367453556058873 62-63 0.02278302585328623 64-65 0.026250008048351522 66-67 0.03801298335303735 68-69 0.03828538909693534 70-71 0.037542464340849915 72-73 0.031227603914123842 74-75 0.05044459093820006 76-77 0.04118279564566848 78-79 0.022560148426460605 80-81 0.01805307157287572 82-83 0.016864391963139044 84-85 0.016418637109487794 86-87 0.017632080877760647 88-89 0.016790099487530505 90-91 0.02471463021910832 92-93 0.03588326571892581 94-95 0.031004726487298215 96-97 0.029419820340982655 98-99 0.031400953023877104 100 0.03249057599946906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2019047.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.4944895102877 #Duplication Level Percentage of deduplicated Percentage of total 1 92.86432016967272 46.89136440687538 2 3.656662246509916 3.6928258689812004 3 1.2249934238098974 1.8556625276622087 4 0.5576689758623449 1.1263684100757625 5 0.33500428828180473 0.8457935260273491 6 0.21619870319034276 0.6550105890229541 7 0.1626980468055658 0.5750748372437557 8 0.10996859003326094 0.44422462527164985 9 0.08659603408090548 0.39353602810777183 >10 0.6308542322887315 6.283177593114732 >50 0.07240374743105533 2.571595898852741 >100 0.06504241009892192 6.926356849768517 >500 0.009761001787867953 3.4805886191563133 >1k 0.005218753431137322 5.333902995499739 >5k 8.697922385228869E-4 3.269090791280375 >10k+ 0.0017395844770457737 15.655426433059516 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 56609 2.803748501149305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 36008 1.783415641141588 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23565 1.1671347918101955 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19467 0.9641677484476587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17495 0.8664979071809621 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 16946 0.8393068611082357 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15469 0.766153536792358 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 14952 0.7405473968659472 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14816 0.733811545744106 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14739 0.7299978653295341 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13147 0.6511487845503349 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13067 0.647186519184546 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12010 0.59483508803906 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11025 0.5460496957227841 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10823 0.5360449756741671 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10333 0.51177610030871 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10322 0.511231288820914 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 10017 0.4961251521138438 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 9150 0.45318410121210656 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8584 0.42515107374914995 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8139 0.4031109726519492 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 8039 0.398158140944713 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7732 0.3829529476034981 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6720 0.3328302907262684 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6705 0.33208736597018296 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6102 0.30222179077554906 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5819 0.2882052770440708 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4845 0.23996469621559083 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4821 0.23877601660585415 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 4578 0.22674063555727034 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4070 0.20158025048451078 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3638 0.18018401750925064 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3537 0.17518165748494216 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3438 0.17027835409477837 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3211 0.15903542611935234 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3065 0.1518042918267876 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2997 0.148436366265867 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2801 0.13872881611968418 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2767 0.1370448533392239 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2687 0.133082587973435 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2522 0.12491041565649537 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 2500 0.12382079268090342 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2403 0.11901654592488435 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2147 0.10633729675435985 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2069 0.10247408802271567 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC 2062 0.10212738980320915 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 2043 0.10118635177883428 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.9528317072361365E-5 4.9528317072361365E-5 2 0.0 0.0 0.0 1.9811326828944546E-4 4.9528317072361365E-5 3 0.0 0.0 0.0 3.466982195065296E-4 4.9528317072361365E-5 4 0.0 0.0 0.0 4.952831707236136E-4 4.9528317072361365E-5 5 0.0 0.0 0.0 5.44811487795975E-4 4.9528317072361365E-5 6 0.0 0.0 0.0 6.438681219406978E-4 4.9528317072361365E-5 7 0.0 0.0 0.0 7.429247560854205E-4 4.9528317072361365E-5 8 0.0 0.0 0.0 8.915097073025046E-4 9.905663414472273E-5 9 0.0 0.0 0.0 0.0014363211950984796 9.905663414472273E-5 10-11 0.0 0.0 0.0 0.001956368524358274 9.905663414472273E-5 12-13 4.9528317072361365E-5 0.0 0.0 0.00255070832922661 1.485849512170841E-4 14-15 9.905663414472273E-5 0.0 0.0 0.0028478782316607785 1.485849512170841E-4 16-17 9.905663414472273E-5 0.0 0.0 0.0035908029877461988 1.485849512170841E-4 18-19 9.905663414472273E-5 0.0 0.0 0.0053490582438150275 1.485849512170841E-4 20-21 1.238207926809034E-4 0.0 0.0 0.020826657328927956 1.485849512170841E-4 22-23 1.485849512170841E-4 0.0 0.0 0.08043398692551486 2.476415853618068E-4 24-25 1.485849512170841E-4 0.0 0.0 0.18110029137508935 2.476415853618068E-4 26-27 1.485849512170841E-4 0.0 0.0 0.3452123699943587 2.476415853618068E-4 28-29 1.485849512170841E-4 0.0 0.0 0.806048596194145 2.476415853618068E-4 30-31 1.9811326828944546E-4 0.0 0.0 1.60905120088834 2.476415853618068E-4 32-33 1.9811326828944546E-4 0.0 0.0 2.8215291669782827 2.476415853618068E-4 34-35 1.9811326828944546E-4 0.0 0.0 4.461486037719776 2.476415853618068E-4 36-37 1.9811326828944546E-4 0.0 0.0 6.186755434618412 2.476415853618068E-4 38-39 2.476415853618068E-4 0.0 0.0 8.054542563892767 2.476415853618068E-4 40-41 2.971699024341682E-4 0.0 0.0 9.87676364146055 2.476415853618068E-4 42-43 2.971699024341682E-4 0.0 0.0 11.37358367586292 2.476415853618068E-4 44-45 2.971699024341682E-4 0.0 0.0 12.84360889072914 2.476415853618068E-4 46-47 2.971699024341682E-4 0.0 0.0 14.43314593469097 2.971699024341682E-4 48-49 2.971699024341682E-4 4.9528317072361365E-5 0.0 15.661621547195285 2.971699024341682E-4 50-51 3.219340609703489E-4 7.429247560854205E-5 0.0 16.74532588889709 2.971699024341682E-4 52-53 3.466982195065296E-4 1.485849512170841E-4 0.0 17.644685834455565 2.971699024341682E-4 54-55 3.466982195065296E-4 1.485849512170841E-4 0.0 18.569181400928258 2.971699024341682E-4 56-57 3.466982195065296E-4 1.485849512170841E-4 0.0 19.746964780909014 2.971699024341682E-4 58-59 3.466982195065296E-4 1.9811326828944546E-4 0.0 20.529536954810858 3.219340609703489E-4 60-61 3.466982195065296E-4 1.9811326828944546E-4 0.0 21.29341218901789 3.466982195065296E-4 62-63 3.7146237804271023E-4 1.9811326828944546E-4 0.0 21.967294471104438 3.466982195065296E-4 64-65 3.962265365788909E-4 1.9811326828944546E-4 0.0 22.579835932496866 3.466982195065296E-4 66-67 3.962265365788909E-4 1.9811326828944546E-4 0.0 23.298392756582686 3.466982195065296E-4 68-69 3.962265365788909E-4 1.9811326828944546E-4 0.0 24.10119724800859 3.466982195065296E-4 70-71 3.962265365788909E-4 1.9811326828944546E-4 0.0 24.86158073586202 3.466982195065296E-4 72-73 3.962265365788909E-4 1.9811326828944546E-4 0.0 25.437099780242857 3.466982195065296E-4 74-75 3.962265365788909E-4 1.9811326828944546E-4 0.0 26.109025693805044 4.209906951150716E-4 76-77 3.962265365788909E-4 1.9811326828944546E-4 0.0 26.61968740697963 5.44811487795975E-4 78-79 3.962265365788909E-4 1.9811326828944546E-4 7.429247560854205E-5 27.436161713917507 5.44811487795975E-4 80-81 3.962265365788909E-4 2.476415853618068E-4 9.905663414472273E-5 28.139612401296255 5.943398048683364E-4 82-83 3.962265365788909E-4 2.971699024341682E-4 9.905663414472273E-5 28.686479314250732 5.943398048683364E-4 84-85 3.962265365788909E-4 2.971699024341682E-4 9.905663414472273E-5 29.276832089594745 5.943398048683364E-4 86-87 3.962265365788909E-4 2.971699024341682E-4 9.905663414472273E-5 29.79056455842781 5.943398048683364E-4 88 3.962265365788909E-4 2.971699024341682E-4 9.905663414472273E-5 30.233025778993753 5.943398048683364E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 1175 0.0 54.423832 1 ACCCATG 1440 0.0 42.775677 3 TACCCAT 1745 0.0 36.37696 2 GTATATG 705 0.0 28.012266 1 CATTAAA 1435 0.0 25.214931 94 TATATGG 730 0.0 25.12059 2 ATCATTA 2320 0.0 24.913576 94 ATATGGG 765 0.0 24.58593 3 TCATTAA 1865 0.0 23.432741 94 TATGGGA 725 0.0 23.348154 4 AGTAAAT 720 0.0 22.857231 2 GTATTAG 215 4.9272785E-8 21.870043 1 CATGGGG 2730 0.0 21.701813 4 GAGTACT 16465 0.0 21.409937 12-13 ATGGGAG 710 0.0 20.529608 5 AGTACTT 16310 0.0 20.475096 12-13 ATAAGCA 1780 0.0 20.339863 5 GTAAATG 840 0.0 20.151682 3 AATAGTG 235 1.2611781E-7 20.008266 5 GTATCAT 3440 0.0 19.80745 94 >>END_MODULE