FastQCFastQC Report
Mon 6 Jun 2016
ERR523090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5884059
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260780.4431974594408384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213430.3627257986366214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133690.22720710312388098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105860.1799098207546865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG115350.060.0073782
ACCTGGG131300.052.3610273
GTACCTG136150.051.4375651
CCTGGGG112100.041.820854
CTGGGGG69850.032.5136385
TATAACG7700.031.1453952
TAACGCA8450.030.6061924
ATAACGC8050.030.376173
TGGGGGG124800.030.0125336
TATCACG4150.028.3273752
GTATCAA289200.027.7286951
GGTATCA201350.027.470021
ATGGGGG106650.027.0702515
GTACCCG11400.026.81711
CATGGGG166100.026.0448284
GTACATG261200.026.0014361
TACATGG268750.024.8458732
ACATGGG271850.023.8712563
TCAACGC332350.023.854224
CAACGCA335300.023.5732235
Produced by FastQC (version 0.11.5)